For the best experience on the Abcam website please upgrade to a modern browser such as
Rabbit polyclonal to CCDC77
antigen sequence, corresponding to amino acids 401-485 (IMTKRYEALE RRRILEVEGF KTDIKVLRQK LKDLEQMLYK ATVNARANQD LALLCEVRDS NRRAHKIQGE LKNLKSKVFG LENEL) of Human CCDC77.
Human lateral ventricle tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Information by UniProt
Immunocytochemistry/ Immunofluorescence - Anti-CCDC77 antibody (ab122513)
Immunofluorescent staining of Human cell line A-431 shows positivity in nuclear membrane. Recommended concentration of ab122513 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC77 antibody (ab122513)
ab122513 at 1/250 dilution staining CCDC77 in Paraffin-embedded Human lateral ventricle tissue by Immunohistochemistry.
Western blot - Anti-CCDC77 antibody (ab122513)
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"