Overview

  • Product name
  • Description
    Rabbit polyclonal to CCDC8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CCDC8 aa 381-461.
    Sequence:

    DNHREEAADNQRAEAPADQGSEVTDNQREEAVHDQRERAPAVQGADNQRA QARAGQRAEAAHNQRAGAPGIQEAEVSAAQG


    Database link: Q9H0W5

  • Positive control
    • IHC-P: Human kidney tissue. ICC/IF: A431 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCDC8 antibody
    • CCDC8_HUMAN antibody
    • Coiled coil domain containing 8 antibody
    • Coiled-coil domain-containing protein 8 antibody
    • DKFZp564K0322 antibody
    • ENSMUSG00000041117 antibody
    • MGC72567 antibody
    see all

Images

  • PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for CCDC8 (green) using ab223784 at 4 µg/ml in ICC/IF.

  • Paraffin-embedded human kidney tissue stained for CCDC8 using ab223784 at 1/200 dilution in immunohistochemical analysis.

References

ab223784 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223784.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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