Overview

  • Product name

  • Description

    Rabbit polyclonal to CCDC80
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, ICC, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from C terminal domain of human CCD80

Properties

Applications

Our Abpromise guarantee covers the use of ab75881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/200 - 1/2000. Predicted molecular weight: 108 kDa.
ICC Use at an assay dependent concentration.
IHC-P 1/50 - 1/500.

Target

  • Function

    Promotes cell adhesion and matrix assembly.
  • Tissue specificity

    Expressed in dermal papilla and dermal fibroblasts (at protein level). Expressed in heart, thymus, placenta, pancreas, colon, epithelium, spleen and osteoblasts.
  • Sequence similarities

    Belongs to the CCDC80 family.
  • Post-translational
    modifications

    Phosphorylated.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2610001E17Rik antibody
    • CCD50 antibody
    • CCD80_HUMAN antibody
    • CCDC80 antibody
    • CL2 antibody
    • Coiled coil domain containing 80 antibody
    • Coiled coil domain containing protein 50 antibody
    • Coiled-coil domain-containing protein 80 antibody
    • Down regulated by oncogenes protein 1 antibody
    • Down-regulated by oncogenes protein 1 antibody
    • DRO1 antibody
    • MGC131805 antibody
    • MGC134851 antibody
    • Nuclear envelope protein okuribin antibody
    • okuribin antibody
    • OTTMUSP00000030743 antibody
    • Protein Ymer antibody
    • SSG1 antibody
    • steroid sensitive gene 1 antibody
    • steroid sensitive protein 1 antibody
    • Up-regulated in BRS-3 deficient mouse homolog antibody
    • URB antibody
    see all

Images

  • ICC/IF image of ab75881 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75881, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Sasagawa S  et al. Comparative Transcriptome Analysis Identifies CCDC80 as a Novel Gene Associated with Pulmonary Arterial Hypertension. Front Pharmacol 7:142 (2016). IHC . Read more (PubMed: 27375481) »
See 1 Publication for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer



Leider kann ich Ihnen diese Information nicht zukommen lassen, da Kunden- und Zuliefererdaten geheim sind.

Wenn Sie mir allerdings eine Liste mit den Firmennamen zukommen lassen, deren Antikörper Sie bereits verwendet haben, kann ich Ihnen aber gerne je nachdem ab- oder zuraten, unser Produkt zu kaufen...

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Answer

Vielen Dank für Ihren Anruf.

Ich habe im Labor nachgefragt, und es sieht so aus, als ob uns gleich zwei Fehler unterlaufen sind: Natürlich muss der Antikörper Anti-CCDC80 und nicht Anti-CCD80 heißen. Desweiteren ist der Zusatz über das 38kDa Protein schlichtweg falsch. Wir wissen auch nicht, wie diese Info auf das Datenblatt gekommen ist. Daher werden wir unser Datenblatt dementsprechend aktualisieren. Vielen Danke dafür, dass Sie uns auf diese Fehler aufmerksam gemacht haben.

Bezüglich eines Bildes muss ich Sie allerdings enttäuschen: Leider können wir Ihnen kein Bild zusenden, allerdings wurde mir versichert, dass dieser Antikörper im WB sowohl an transfizierten Zellen als auch an humanen Lungen Epithelzellen getestet wurde.

Allerdings haben wir eine 6 Monate Garantie, unser Abpromise, auf unsere Produkte, wenn die Spezies und die Applikation auf unserem Datenblatt als getestet ausgewiesen wurden, und wenn der Antikörper wie auf unserem Datenblatt angeben korrekt gelagert wurde. Das heißt, dass wir uns bei Problemen das Protokoll und die Ergebnisse unserer Kunden anshene, um dann gegebenenfalls Verbesserungsvorschläge zu machen. Falls das Protokoll bereits optimiert wurde, oder falls diese Tipps nicht funktionieren, senden wir Ihnen gerne einen kostenlosen Ersatz zu oder stellen eine Gutschrift oder eine Rückerstattung aus.


Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (lung epithelial cells)
Specification
lung epithelial cells
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton x-100 0.2%
Blocking step
PBS 3%BSA, 0.4% Tritonx-100 as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C

Abcam user community

Verified customer

Submitted May 16 2012

Answer

Thank you for contacting us.
Yes, that would be no problem at all. I have now added the discount code to your review.
Since the review has been submitted, you can go ahead and use the discount code on your next order.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
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Answer

I am very pleased to hear you would like to accept our offer and test ab75881 in mouse tissues. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you very much for your interest in ab75881.

Ab75881 has been predicted to work in Mouse and Rat as the protein sequences of those species contains over 85% homology with the synthetic peptide derived from C terminal domain of human CCD80. Because that peptide sequence is considered proprietary I am unable to share that at the moment.

To our knowledge this product has not been tested in mouse and we cannot therefore guarantee its effectiveness. However if you would like to try this product in mouse samples and submit an Abreview of your results I could enroll you in our 100% Abreview Discount program. Under this program I can offer a discount off a future purchase if you buy ab75881 now, test it in mouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab75881 in mouse. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering.

2. Purchase ab75881 either by phone, fax, or online (www.abcam.com).

3. Test it in mouse.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab75881 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted.


Please let me know if you have any questions about this offer and I would be happy to help you further.



The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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