Product nameAnti-CCDC97 antibody
See all CCDC97 primary antibodies
DescriptionRabbit polyclonal to CCDC97
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Sheep, Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan
Synthetic peptide within Human CCDC97 aa 293-343. The exact sequence is proprietary. (NP_443080.1).
Database link: Q96F63
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225997 was affinity purified using an epitope specific to CCDC97 immobilized on solid support.
Our Abpromise guarantee covers the use of ab225997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 39 kDa.|
- Information by UniProt
- CCD97_HUMAN antibody
- CCDC97 antibody
- Coiled coil domain containing 97 antibody
All lanes : Anti-CCDC97 antibody (ab225997) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 39 kDa
Exposure time: 30 seconds
CCDC97 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225997 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225997 at 1 µg/ml.
Lane 1: ab225997 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab225997 has not yet been referenced specifically in any publications.