Overview

  • Product name

    Anti-CCDC98 antibody [EPR6310(2)]
    See all CCDC98 primary antibodies
  • Description

    Rabbit monoclonal [EPR6310(2)] to CCDC98
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CCDC98 aa 350-450 (C terminal). The exact sequence is proprietary.
    Database link: Q6UWZ7

  • Positive control

    • HeLa, MCF7, 293T and Jurkat whole cell lysate (ab7899); HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab139191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as a central scaffold protein that assembles the various components of the BRCA1-A complex and mediates the recruitment of BRCA1.
    • Sequence similarities

      Belongs to the FAM175 family. Abraxas subfamily.
    • Domain

      The MPN-like region is similar to the MPN (JAB/Mov34) domain. Its presence reveals similarities between the structure of the 19S proteasome and the BRCA1-A complexes.
    • Post-translational
      modifications

      Phosphorylation of Ser-406 of the pSXXF motif by ATM or ATR constitutes a specific recognition motif for the BRCT domain of BRCA1. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus. Localizes at sites of DNA damage at double-strand breaks.
    • Information by UniProt
    • Database links

    • Alternative names

      • 3830405G04Rik antibody
      • 5630400M01Rik antibody
      • ABRA1 antibody
      • ABRAXAS antibody
      • Abraxas protein antibody
      • AI506069 antibody
      • AL024423 antibody
      • AV118690 antibody
      • BRCA1-A complex subunit Abraxas antibody
      • coiled coil domain containing 98 antibody
      • Coiled-coil domain-containing protein 98 antibody
      • F175A_HUMAN antibody
      • FAM175A antibody
      • Family with sequence similarity 175 member A antibody
      • FLJ11520 antibody
      • FLJ12642 antibody
      • FLJ13614 antibody
      • Protein FAM175A antibody
      see all

    Images

    • All lanes : Anti-CCDC98 antibody [EPR6310(2)] (ab139191) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 47 kDa

    • Overlay histogram showing HeLa cells stained with ab139191 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab139191, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Immunofluorescent analysis of HeLa cells labelling CCDC98 with ab139191 at 1/100 dilution.

    References

    This product has been referenced in:

    • Anantha RW  et al. Functional and mutational landscapes of BRCA1 for homology-directed repair and therapy resistance. Elife 6:N/A (2017). Read more (PubMed: 28398198) »
    • Leung JW  et al. ZMYM3 regulates BRCA1 localization at damaged chromatin to promote DNA repair. Genes Dev 31:260-274 (2017). Read more (PubMed: 28242625) »
    See all 2 Publications for this product

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