Product nameCCK Octapeptide sulfated, C-terminal ocatapeptide of CCK
DescriptionC-terminal ocatapeptide of CCK
- Cholecystokinin Octapeptide sulfated
SequenceDYMGWMDF (Modifications: C-terminal amide; Tyr-2 = Sulfonylation)
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in 0.1% NH4OH to 1mg/ml
This product is supplied in one (or more) pack size which is freeze dried. Therefore the contents may not be readily visible, as they can coat the bottom or walls of the vial. Please see our FAQs and information page for more details on handling.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab120209 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab40794 staining FAK in PANC-1 cells treated with CCK Octapeptide sulfated (ab120209), by ICC/IF. Increase of FAK expression correlates with increased concentration of CCK Octapeptide sulfated, as described in literature.
The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120209 (CCK Octapeptide sulfated) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab40794 (1/200) dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab120209 has been referenced in 1 publication.
- Yu Y et al. Inducible nitric oxide synthase-derived nitric oxide reduces vagal satiety signalling in obese mice. J Physiol N/A:N/A (2018). PubMed: 30565225