Overview

  • Product name
  • Description
    Goat polyclonal to CCK1-R
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human CCK1-R aa 242-257 (internal sequence).
    Sequence:

    C-FEASQKKSAKERKPST


    Database link: NP_000721.1

  • Positive control
    • Human Pancreas tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: CaCo2
  • General notes

    Protein previously labeled as CCKAR.

Properties

Applications

Our Abpromise guarantee covers the use of ab77269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration. Antibody detection limit for peptide ELISA 1:4000
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCK A antibody
    • CCK A receptor antibody
    • CCK AR antibody
    • CCK-A receptor antibody
    • CCK-AR antibody
    • CCK1 R antibody
    • CCK1-R antibody
    • CCK1R antibody
    • CCKA antibody
    • CCKA receptor antibody
    • CCKAR antibody
    • CCKAR_HUMAN antibody
    • CCKR Type A antibody
    • CCKRA antibody
    • Cholecystokinin 1 receptor antibody
    • Cholecystokinin A receptor antibody
    • Cholecystokinin receptor type A antibody
    • Cholecystokinin type A receptor antibody
    • Cholecystokinin-1 receptor antibody
    see all

Images

  • ab77269 (4 µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

  • ICC/IF image of ab77269 stained CaCo2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab77269 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti-goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab77269 at 10 µg/ml staining CCK1-R in human pancreas by Immunohistochemistry, Paraffin-embedded tissue (note cytoplasmic staining of some cells in the islets of Langerhans).

References

This product has been referenced in:
  • El-Kouhen K & Morisset J Control of somatostatin (SS) secretion by CCK-1 and CCK-2 receptors' occupation in RIN-14B cells, a rat pancreatic islet cell line. Pancreas 39:127-34 (2010). ICC/IF . Read more (PubMed: 19959964) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
Yes - Triton X-100
Specification
Pancreas
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 19°C
Fixative
Formaldehyde
Username

Ms. Alison Holiday

Verified customer

Submitted Feb 07 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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