Anti-CCK1-R antibody (ab77269)
Key features and details
- Goat polyclonal to CCK1-R
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CCK1-R antibody
See all CCK1-R primary antibodies -
Description
Goat polyclonal to CCK1-R -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide corresponding to Human CCK1-R aa 242-257 (internal sequence).
Sequence:C-FEASQKKSAKERKPST
Database link: NP_000721.1 -
General notes
Protein previously labeled as CCKAR.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab77269 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Notes |
|---|
|
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 886 Human
- Omim: 118444 Human
- SwissProt: P32238 Human
- Unigene: 129 Human
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Alternative names
- CCK A antibody
- CCK A receptor antibody
- CCK AR antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCK1-R antibody (ab77269)
ab77269 (4 µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCK1-R antibody (ab77269)ab77269 at 10 µg/ml staining CCK1-R in human pancreas by Immunohistochemistry, Paraffin-embedded tissue (note cytoplasmic staining of some cells in the islets of Langerhans).
Protocols
Datasheets and documents
References (1)
ab77269 has been referenced in 1 publication.
- El-Kouhen K & Morisset J Control of somatostatin (SS) secretion by CCK-1 and CCK-2 receptors' occupation in RIN-14B cells, a rat pancreatic islet cell line. Pancreas 39:127-34 (2010). ICC/IF . PubMed: 19959964
