Anti-CCK1-R antibody (ab77269)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-CCK1-R antibody
See all CCK1-R primary antibodies -
Description
Goat polyclonal to CCK1-R -
Host species
Goat -
Tested applications
Suitable for: IHC-P, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Human CCK1-R aa 242-257 (internal sequence).
Sequence:C-FEASQKKSAKERKPST
Database link: NP_000721.1 -
Positive control
- Human Pancreas tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: CaCo2
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General notes
Protein previously labeled as CCKAR.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab77269 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab77269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ELISA | Use at an assay dependent concentration. Antibody detection limit for peptide ELISA 1:4000 | |
| ICC/IF | Use a concentration of 5 µg/ml. |
Target
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Function
Receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 886 Human
- Entrez Gene: 24889 Rat
- Omim: 118444 Human
- SwissProt: P32238 Human
- SwissProt: P30551 Rat
- Unigene: 129 Human
- Unigene: 10184 Rat
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Alternative names
- CCK A antibody
- CCK A receptor antibody
- CCK AR antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCK1-R antibody (ab77269)ab77269 (4 µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
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Immunocytochemistry/ Immunofluorescence - Anti-CCK1-R antibody (ab77269)ICC/IF image of ab77269 stained CaCo2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab77269 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti-goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCK1-R antibody (ab77269)ab77269 at 10 µg/ml staining CCK1-R in human pancreas by Immunohistochemistry, Paraffin-embedded tissue (note cytoplasmic staining of some cells in the islets of Langerhans).
Protocols
Datasheets and documents
References
This product has been referenced in:
- El-Kouhen K & Morisset J Control of somatostatin (SS) secretion by CCK-1 and CCK-2 receptors' occupation in RIN-14B cells, a rat pancreatic islet cell line. Pancreas 39:127-34 (2010). ICC/IF . Read more (PubMed: 19959964) »
