Product nameAnti-CCK2-R antibody
See all CCK2-R primary antibodies
DescriptionRabbit polyclonal to CCK2-R
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Human thyroid cancer tissue.
Protein previously labeled as CCKBR.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol
PBS (without Mg2+and Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab183124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/5000. Predicted molecular weight: 48 kDa.|
|IHC-P||1/50 - 1/150.|
FunctionReceptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Isoform 2 is constitutively activated and may regulate cancer cell proliferation via a gastrin-independent mechanism.
Tissue specificityIsoform 1 is expressed in brain, pancreas, stomach, the colon cancer cell line LoVo and the T-lymphoblastoma Jurkat, but not in heart, placenta, liver, lung, skeletal muscle, kidney or the stomach cancer cell line AGS. Expressed at high levels in the small cell lung cancer cell line NCI-H510, at lower levels in NCI-H345, NCI-H69 and GLC28 cell lines, not expressed in GLC19 cell line. Within the stomach, expressed at high levels in the mucosa of the gastric fundus and at low levels in the antrum and duodenum. Isoform 2 is present in pancreatic cancer cells and colorectal cancer cells, but not in normal pancreas or colonic mucosa. Isoform 3 is expressed in brain, pancreas, stomach, the stomach cancer cell line AGS and the colon cancer cell line LoVo.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- CCK B antibody
- CCK B receptor antibody
- CCK BR antibody
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling CCK2-R with ab183124 at 1/75 dilution. The image on the right is treated with the synthesized peptide. (Original magnification: X 200)
Anti-CCK2-R antibody (ab183124) at 1/1600 dilution + Human gastric cancer tissue at 40 µg
Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 48 kDa
Exposure time: 10 seconds
ab183124 has been referenced in 2 publications.
- Wu W et al. CCK1 receptor is involved in the regulation of protein lysine acetylation in GBC-SD cells and gallbladder carcinoma. Ir J Med Sci N/A:N/A (2017). WB ; Human . PubMed: 28470351
- Zhou Y et al. Cholecystokinin receptors regulate sperm protein tyrosine phosphorylation via uptake of HCO3-. Reproduction 150:257-68 (2015). PubMed: 26175429