Overview

  • Product name

  • Description

    Goat polyclonal to CCK2-R
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-P, IHC-FoFr, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide corresponding to Human CCK2-R aa 251-266.
    Sequence:

    C-FDGDSDSDSQSRVRNQ


    (Peptide available as ab208978)

  • Positive control

    • Purified membranes of rat insulinoma cell line RIN14B. Human pancreas tissue.
  • General notes

    Protein previously labeled as CCKBR.

Properties

Applications

Our Abpromise guarantee covers the use of ab77077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 48 kDa). The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem. 2003 Nov;51(11):1501-13; PMID: 14566022).
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-FoFr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Receptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
    Isoform 2 is constitutively activated and may regulate cancer cell proliferation via a gastrin-independent mechanism.
  • Tissue specificity

    Isoform 1 is expressed in brain, pancreas, stomach, the colon cancer cell line LoVo and the T-lymphoblastoma Jurkat, but not in heart, placenta, liver, lung, skeletal muscle, kidney or the stomach cancer cell line AGS. Expressed at high levels in the small cell lung cancer cell line NCI-H510, at lower levels in NCI-H345, NCI-H69 and GLC28 cell lines, not expressed in GLC19 cell line. Within the stomach, expressed at high levels in the mucosa of the gastric fundus and at low levels in the antrum and duodenum. Isoform 2 is present in pancreatic cancer cells and colorectal cancer cells, but not in normal pancreas or colonic mucosa. Isoform 3 is expressed in brain, pancreas, stomach, the stomach cancer cell line AGS and the colon cancer cell line LoVo.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CCK B antibody
    • CCK B receptor antibody
    • CCK BR antibody
    • CCK-B receptor antibody
    • CCK-BR antibody
    • CCK2 R antibody
    • CCK2 receptor antibody
    • CCK2-R antibody
    • CCKB antibody
    • CCKB receptor antibody
    • CCKBR antibody
    • CCKR Type B antibody
    • CCKRB antibody
    • Cholecystokinin 2 receptor antibody
    • Cholecystokinin B receptor antibody
    • Cholecystokinin-2 receptor antibody
    • Cholecystokinin2 receptor antibody
    • CholecystokininB receptor antibody
    • GASR antibody
    • GASR_HUMAN antibody
    • Gastrin receptor antibody
    • Gastrin/cholecystokinin type B receptor antibody
    • Gastrin\cholecystokinin brain receptor antibody
    see all

Images

  • ab77077 (4µg/ml) staining of paraffin embedded Human Pancreas tissue. Steamed antigen retrieval carried using Tris/EDTA buffer pH 9 followed by HRP-staining.

  • ab77077, at 3µg/ml, staining CCK2-R in paraffin-embedded Human pancreas tissue. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Note strong staining in islets of Langerhans and weak staining in acinar cells.

References

This product has been referenced in:

  • Smith JP  et al. Cholecystokinin receptor antagonist alters pancreatic cancer microenvironment and increases efficacy of immune checkpoint antibody therapy in mice. Cancer Immunol Immunother 67:195-207 (2018). ICC/IF . Read more (PubMed: 29043413) »
  • Smith JP  et al. Cholecystokinin receptor antagonist halts progression of pancreatic cancer precursor lesions and fibrosis in mice. Pancreas 43:1050-9 (2014). Read more (PubMed: 25058882) »
See all 4 Publications for this product

Customer reviews and Q&As

Question
Answer

Thank you for contacting us.

I have checked the sequence homology of the immunogen of each antibody witht he sequence of the CCK receptor A.
For ab43404, the homology is 0%, and forab77077, the homology isonly 12.5%. Thus, no cross reactivity with the receptor A can be expected.

ab77077 is guaranteed for human samples and IHC-P, while ab43404 has not yet been tested in IHC-P and you would be eligible to use our testing discount program (please see below for more details).

We guarantee all our products for TESTED species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise.

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
This programapplies to ELISA kits and other kits we offer.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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