Overview

  • Product name

    Anti-CCK4 antibody - N-terminal
    See all CCK4 primary antibodies
  • Description

    Rabbit polyclonal to CCK4 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 30-59 of Human CCK4

  • Positive control

    • 293 cell lysates transiently transfected with the CCK4 gene. Human hepatocarcinoma tissue. NCI-H460 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab135675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/200. Predicted molecular weight: 118 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
  • Tissue specificity

    Highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. But not expressed in colon.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Domain

    The protein kinase domain is predicted to be catalytically inactive.
  • Post-translational
    modifications

    MMP14 cleaves PTK7 between Pro-621 and Leu-622 generating an N-terminal soluble (70 kDa) fragment and a membrane C-terminal (50 kDa) fragment. Proteolysis by MMP14 regulates PTK7 function in non-canonical Wnt signaling pathway.
  • Cellular localization

    Membrane. Cell junction. Colocalizes with MMP14 at cell junctions. Also localizes at the leading edge of migrating cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • 8430404F20Rik antibody
    • CCK 4 antibody
    • CCK-4 antibody
    • Colon carcinoma kinase 4 antibody
    • Human colon carcinoma kinase 4 (CCK4) mRNA complete cds antibody
    • Inactive tyrosine-protein kinase 7 antibody
    • mPTK7/CCK4 antibody
    • OTTHUMP00000217016 antibody
    • Protein tyrosine kinase PTK 7 antibody
    • Protein-tyrosine kinase 7 antibody
    • Pseudo tyrosine kinase receptor 7 antibody
    • PTK 7 antibody
    • PTK7 antibody
    • PTK7 protein tyrosine kinase 7 antibody
    • PTK7 protein tyrosine kinase antibody
    • PTK7_HUMAN antibody
    • Serum response factor antibody
    • Srf antibody
    • Tyrosine-protein kinase-like 7 antibody
    see all

Images

  • All lanes : Anti-CCK4 antibody - N-terminal (ab135675) at 1/50 dilution

    Lane 1 : Non-transfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the CCK4 gene

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 118 kDa

  • Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human hepatocarcinoma tissue labelling CCK4 with ab135675 at 1/50 dilution, followed by peroxidase-conjugated to the secondary antibody, and DAB staining.
  • Flow cytometric analysis of NCI-H460 cells labelling CCK4 with ab135675 at 1/10 dilution (green) compared to a negative control cells (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab135675 has not yet been referenced specifically in any publications.

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