Overview

  • Product name
  • Description
    Rabbit polyclonal to CCL14
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant CCL14 (human Hemofiltrate CC Chemokine 1)

  • Positive control
    • Recombinant human CCL14 protein (ab9846) can be used as a positive control in WB.
  • General notes


    CCL14 was first isolated from the hemofiltrate of patients with chronic renal failure. It has a 46% amino acid sequence identity with MIP-1a/MIP-1b and 29%-37% homology with other CC chemokines.

Properties

Applications

Our Abpromise guarantee covers the use of ab9845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml.

To detect CCL14 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant CCL14.

WB Use a concentration of 0.1 - 0.2 µg/ml.

Used in conjunction with compatible secondary reagents the detection limit for recombinant CCL14 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Target

  • Function
    Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.
  • Tissue specificity
    Expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations (1-80 nM) in plasma.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    The N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood.
    HCC-1(1-74), but not HCC-1(3-74) and HCC-1(4-74), is partially O-glycosylated; the O-linked glycan consists of one Gal-GalNAc disaccharide, further modified by two N-acetylneuraminic acids.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CC-1 antibody
    • CC-3 antibody
    • CCL14 antibody
    • CCL14_HUMAN antibody
    • chemokine (C-C motif) ligand 14 antibody
    • Chemokine CC-1/CC-3 antibody
    • chemokine CC1 antibody
    • chemokine CC3 antibody
    • chemokine HCC1 antibody
    • chemokine HCC3 antibody
    • CKb1 antibody
    • HCC-1(1-74) antibody
    • HCC-1(9-74) antibody
    • HCC-1/HCC-3 antibody
    • HCC-3 antibody
    • HCC1 antibody
    • HEMOFILTRATE CC CHEMOKINE 1 antibody
    • MCIF antibody
    • NCC-2 antibody
    • NCC2 antibody
    • new CC chemokine 2 antibody
    • SCYA14 antibody
    • SCYL2 antibody
    • small inducible cytokine subfamily A (Cys-Cys), member 14 antibody
    • Small-inducible cytokine A14 antibody
    • SY14 antibody
    see all

References

This product has been referenced in:
  • Kavanagh Williamson M  et al. Upregulation of Glucose Uptake and Hexokinase Activity of Primary Human CD4+ T Cells in Response to Infection with HIV-1. Viruses 10:N/A (2018). Read more (PubMed: 29518929) »
  • Yin Q  et al. Plasma Long Non-Coding RNA (lncRNA) GAS5 is a New Biomarker for Coronary Artery Disease. Med Sci Monit 23:6042-6048 (2017). Read more (PubMed: 29267258) »
See all 9 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry. First, I'm pleased to hear that ab12326 is working out for you in Wetern blotting. Ab12326 has not yet been tested for application in IP. I do suggest that you submit a review regarding your experiences with the antibody - in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards. As for ab9845, it is a polyclonal antibody and will therefore consist of a mixed population of IgGs each of which will recognise a different epitope. To our knowledge, ab9845 has only been tested for application in Western blotting and ELISA, and has not yet been tesed in IP or ChIP. If you have any additional questions, please contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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