Overview

  • Product name
  • Description
    Rabbit polyclonal to CCL18
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, Neutralisingmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to CCL18.

  • Positive control
    • Recombinant human CCL18 protein (ab9850) can be used as a positive control in WB.

Properties

  • Form
    Lyophilised:Reconstitute with 200µl of sterile water.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    unknown
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
Neutralising
  • Application notes
    Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-4 (100 ng/ml), a concentration of 8.0 - 10.0 µg/ml of this antibody is required.

    ELISA:To detect hMIP-4 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-4.

    Western Blot:To detect hMIP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • Target

    • Function
      Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses.
    • Tissue specificity
      Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid.
    • Sequence similarities
      Belongs to the intercrine beta (chemokine CC) family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alternative macrophage activation associated CC chemokine 1 antibody
      • Alternative macrophage activation-associated CC chemokine 1 antibody
      • AMAC-1 antibody
      • AMAC1 antibody
      • CC chemokine PARC antibody
      • CCL18 antibody
      • CCL18(4-69) antibody
      • CCL18_HUMAN antibody
      • CKb7 antibody
      • DC-CK1 antibody
      • DCCK1 antibody
      • DCCK1 antibody
      • Dendritic cell chemokine 1 antibody
      • Macrophage inflammatory protein 4 antibody
      • MIP-4 antibody
      • MIP4 antibody
      • PARC antibody
      • Pulmonary and activation regulated chemokine antibody
      • Pulmonary and activation-regulated chemokine antibody
      • SCYA18 antibody
      • Small inducible cytokine A18 antibody
      • Small inducible cytokine A18 antibody
      • Small-inducible cytokine A18 antibody
      see all

    References

    This product has been referenced in:
    • Su S  et al. Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer. Cell Res 27:461-482 (2017). Blocking ; Human . Read more (PubMed: 28290464) »
    • Su S  et al. A positive feedback loop between mesenchymal-like cancer cells and macrophages is essential to breast cancer metastasis. Cancer Cell 25:605-20 (2014). Read more (PubMed: 24823638) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you for contacting us.

    The isotype of ab9849 is rabbit IgG so you could choose a rabbit IgG antibody as isotype control in neutralising experiments.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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