Overview

  • Product name

    Anti-CCL21 antibody [MM0146-2H26]
    See all CCL21 primary antibodies
  • Description

    Mouse monoclonal [MM0146-2H26] to CCL21
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length Human protein.

  • General notes

    Reconstituted antibody should be aliquoted and can be stored frozen at < -20 for at least six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Properties

  • Form

    Lyophilised:Reconstitute the antibody with 200 µl sterile PBS to give a final concentration of 0.5 mg/ml.
  • Storage instructions

    Shipped at 4°C. Store at -80°C.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and filtered through a 0.2 µm filter.
  • Clonality

    Monoclonal
  • Clone number

    MM0146-2H26
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 15 kDa.

Target

  • Function

    Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs.
  • Tissue specificity

    Highly expressed in high endothelial venules of lymph nodes, spleen and appendix. Intermediate levels found in small intestine, thyroid gland and trachea. Low level expression in thymus, bone marrow, liver, and pancreas. Also found in tonsil, fetal heart and fetal spleen.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • 6Ckine antibody
    • Beta chemokine exodus 2 antibody
    • Beta-chemokine exodus-2 antibody
    • C C motif chemokine ligand 21 antibody
    • C-C motif chemokine 21 antibody
    • CCL21 antibody
    • CCL21_HUMAN antibody
    • Chemokine (C-C motif) ligand 21 antibody
    • Chemokine CC motif ligand 21 antibody
    • CKb9 antibody
    • ECL antibody
    • Efficient Chemoattractant for Lymphocytes antibody
    • SCYA21 antibody
    • Secondary lymphoid tissue chemokine antibody
    • Secondary lymphoid-tissue chemokine antibody
    • SLC antibody
    • Small inducible cytokine A21 antibody
    • Small inducible cytokine subfamily A (Cys-Cys), member 21 antibody
    • Small-inducible cytokine A21 antibody
    • TCA4 antibody
    • UNQ784/PRO1600 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4µm) labelling CCL21 with ab89396. Tissue was treated with 15µg/ml Protenase K for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.

References

ab89396 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

We currently have six anti-CCL21 antibodies in our catalog. Of these, four are polyclonal (3 rabbit, 1 chicken), one is mouse monoclonal (ab89396) and one is rabbit monoclonal (ab129014). Our mouse monoclonal antibody, ab89396 has, to our knowledge, only been tested with recominent fragement. Our rabbit monoclonal, ab129014, has been tested against Caco 2 cells. More information about this product may be found at: https://www.abcam.com/CCL21-antibody-EPR6218-ab129014.html

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