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Immunology Innate Immunity Macrophage / Inflamm.
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RecombinantRabMAb

Recombinant Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)
  • Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11363] to CCL23 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-CCL23 antibody [EPR11363] - BSA and Azide free
    See all CCL23 primary antibodies
  • Description

    Rabbit monoclonal [EPR11363] to CCL23 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249653 is the carrier-free version of ab171751. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab249653 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11363
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokines (CC)
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits

Associated products

  • Alternative Versions

    • Anti-CCL23 antibody [EPR11363] (ab171751)
  • Conjugation kits

    • FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Recombinant Protein

    • Recombinant human CCL23 protein (Active) (ab9931)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab249653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23.
    • Tissue specificity

      High levels in adult lung, liver, skeletal muscle and pancreas. Moderate levels in fetal liver, adult bone marrow and placenta. The short form is the major species and the longer form was detected only in very low abundance. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are found in high levels in synovial fluids from rheumatoid patients.
    • Sequence similarities

      Belongs to the intercrine beta (chemokine CC) family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P55773 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6368 Human
      • Omim: 602494 Human
      • SwissProt: P55773 Human
      • Unigene: 169191 Human
      • Alternative names

        • C C motif chemokine ligand 23 antibody
        • C-C motif chemokine 23 antibody
        • C6 beta chemokine antibody
        • CCL 23 antibody
        • CCL23 antibody
        • CCL23(30-99) antibody
        • CCL23_HUMAN antibody
        • Chemokine (C C motif) ligand 23 antibody
        • Chemokine CC motif ligand 23 antibody
        • ck beta 8 antibody
        • CK-beta-8 antibody
        • CKB 8 antibody
        • CKB-8 antibody
        • Ckb-8-1 antibody
        • CKB8 antibody
        • Hmrp-2a antibody
        • Macrophage inflammatory protein 3 antibody
        • MIP 3 antibody
        • MIP-3 antibody
        • MIP3 antibody
        • MPIF 1 antibody
        • MPIF-1 antibody
        • MPIF1 antibody
        • Myeloid progenitor inhibitory factor 1 antibody
        • OTTHUMP00000163948 antibody
        • SCYA 23 antibody
        • SCYA23 antibody
        • Small inducible cytokine A 23 antibody
        • Small inducible cytokine subfamily A (Cys-Cys), member 23 antibody
        • Small inducible cytokine subfamily A, member 23 antibody
        • Small-inducible cytokine A23 antibody
        see all

      Images

      • Western blot - Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)
        Western blot - Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)
        Anti-CCL23 antibody [EPR11363] (ab171751) at 1/1000 dilution + Human recombinant CCL23 at 0.005 µg

        Predicted band size: 13 kDa



        This data was developed using ab171751, the same antibody clone in a different buffer formulation.

        Note: Primary antibody diluted in 1% BSA.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)
        This data was developed using ab171751, the same antibody clone in a different buffer formulation.Immunohistochemistry of paraffin-embedded Human pancreas tissue labeling CCL23 using ab171751 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)
        This data was developed using ab171751, the same antibody clone in a different buffer formulation.Immunohistochemistry of paraffin-embedded Human liver tissue labeling CCL23 using ab171751 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)
        Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab249653? Please let us know so that we can cite the reference in this datasheet.

      ab249653 has not yet been referenced specifically in any publications.

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