Recombinant Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)
![Western blot - Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)](https://www.abcam.com/ps/products/249/ab249653/Images/ab249653-406020-anti-ccl23-antibody-epr11363-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11363] to CCL23 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
-
Product name
Anti-CCL23 antibody [EPR11363] - BSA and Azide free
See all CCL23 primary antibodies -
Description
Rabbit monoclonal [EPR11363] to CCL23 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab249653 is the carrier-free version of ab171751. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab249653 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11363 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.
|
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa. |
Target
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Function
Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23. -
Tissue specificity
High levels in adult lung, liver, skeletal muscle and pancreas. Moderate levels in fetal liver, adult bone marrow and placenta. The short form is the major species and the longer form was detected only in very low abundance. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are found in high levels in synovial fluids from rheumatoid patients. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6368 Human
- Omim: 602494 Human
- SwissProt: P55773 Human
- Unigene: 169191 Human
-
Alternative names
- C C motif chemokine ligand 23 antibody
- C-C motif chemokine 23 antibody
- C6 beta chemokine antibody
see all
Images
-
Western blot - Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)](https://www.abcam.com/ps/products/249/ab249653/Images/ab249653-2-anti-ccl23-antibody-epr11363-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)This data was developed using ab171751, the same antibody clone in a different buffer formulation.Immunohistochemistry of paraffin-embedded Human pancreas tissue labeling CCL23 using ab171751 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)](https://www.abcam.com/ps/products/249/ab249653/Images/ab249653-1-anti-ccl23-antibody-epr11363-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)This data was developed using ab171751, the same antibody clone in a different buffer formulation.Immunohistochemistry of paraffin-embedded Human liver tissue labeling CCL23 using ab171751 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)](https://www.abcam.com/ps/products/249/ab249653/Images/ab249653-4-benefits-of-recombinant-antibodies.png)
Anti-CCL23 antibody [EPR11363] - BSA and Azide free (ab249653)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab249653 has not yet been referenced specifically in any publications.
