Overview

  • Product name

  • Description

    Rabbit polyclonal to CCL25
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, Neutralising, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hTECK (human Thymus Expressed Chemokine)

  • Positive control

    • Recombinant human CCL25 protein (ab586) can be used as a positive control in WB.
  • General notes

     This product was previously labelled as TECK

     

Properties

  • Form

    Lyophilised:Reconstitute with 200µl of sterile water.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    unknown
  • Light chain type

    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect hTECK by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTECK.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hTECK (100.00 ng/ml), a concentration of 10.0 - 15.0 µg/ml of this antibody is required.
Sandwich ELISA Use a concentration of 0.5 - 1 µg/ml.

Assuming 100µl/well, a concentration of 0.5-2.0 µg/ml of ab2164 will detect at least 0.2-0.4 ng/well of recombinant human CCL25 when used with ab83146 as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.

Target

  • Function

    Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1.
  • Tissue specificity

    Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • A130072A22Rik antibody
    • C-C motif chemokine 25 antibody
    • CCL 25 antibody
    • CCL25 antibody
    • CCL25_HUMAN antibody
    • Chemokine (C C motif) ligand 25 antibody
    • Chemokine (CC motif) ligand 25 antibody
    • Chemokine C C motif ligand 25 antibody
    • Chemokine CC motif ligand 25 antibody
    • Chemokine TECK antibody
    • Ck beta 15 antibody
    • Ckb 15 antibody
    • Ckb15 antibody
    • MGC125074 antibody
    • MGC150327 antibody
    • SCY A25 antibody
    • SCYA 25 antibody
    • SCYA25 antibody
    • Small inducible cytokine A25 antibody
    • Small inducible cytokine A25 isoform 1 precursor antibody
    • Small inducible cytokine A25 isoform 2 precursor antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 25 antibody
    • Small inducible cytokine subfamily A Cys Cys member 25 antibody
    • Small inducible cytokine subfamily A, member 25 antibody
    • Small-inducible cytokine A25 antibody
    • TECK antibody
    • TECKvar antibody
    • Thymus expressed chemokine antibody
    • Thymus-expressed chemokine antibody
    see all

References

ab2164 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab2164.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up