Overview

  • Product name

    Anti-CCL25 antibody [EPR12388(2)]
    See all CCL25 primary antibodies
  • Description

    Rabbit monoclonal [EPR12388(2)] to CCL25
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    within Human CCL25 aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: O15444

  • Positive control

    • WB: Human thymus lysate. IHC-P: Human thymus and small intestine tissue.
  • General notes

     This product was previously labelled as TECK

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12388(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab200343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Predicted molecular weight: 17 kDa.
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1.
  • Tissue specificity

    Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • A130072A22Rik antibody
    • C-C motif chemokine 25 antibody
    • CCL 25 antibody
    • CCL25 antibody
    • CCL25_HUMAN antibody
    • Chemokine (C C motif) ligand 25 antibody
    • Chemokine (CC motif) ligand 25 antibody
    • Chemokine C C motif ligand 25 antibody
    • Chemokine CC motif ligand 25 antibody
    • Chemokine TECK antibody
    • Ck beta 15 antibody
    • Ckb 15 antibody
    • Ckb15 antibody
    • MGC125074 antibody
    • MGC150327 antibody
    • SCY A25 antibody
    • SCYA 25 antibody
    • SCYA25 antibody
    • Small inducible cytokine A25 antibody
    • Small inducible cytokine A25 isoform 1 precursor antibody
    • Small inducible cytokine A25 isoform 2 precursor antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 25 antibody
    • Small inducible cytokine subfamily A Cys Cys member 25 antibody
    • Small inducible cytokine subfamily A, member 25 antibody
    • Small-inducible cytokine A25 antibody
    • TECK antibody
    • TECKvar antibody
    • Thymus expressed chemokine antibody
    • Thymus-expressed chemokine antibody
    see all

Images

  • All lanes : Anti-CCL25 antibody [EPR12388(2)] (ab200343) at 1/10000 dilution

    Lane 1 : Human thymus lysate
    Lane 2 : Human fetal lung lysate
    Lane 3 : Human fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa



    5% NFDM/TBST: Blocking and diluting buffer.

  • Immunohistochemical analysis of paraffin-embedded human thymus tissue labeling CCL25 using ab200343 at 1/250 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab200343 and secondary antibody only.
    Note: Cytoplasm staining on human thymus tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue labeling CCL25 using ab200343 at 1/250 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab200343 and secondary antibody only.
    Note: Cytoplasm staining on human small intestine tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CCL25 using ab200343 at 1/250 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab200343 and secondary antibody only.
    Note: No staining on human colon tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab200343 has not yet been referenced specifically in any publications.

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