Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8582(2)] to CCL27
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-CCL27 antibody [EPR8582(2)]
See all CCL27 primary antibodies
DescriptionRabbit monoclonal [EPR8582(2)] to CCL27
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CCL27 aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: Q9Y4X3
- Human CCL27 recombinant protein; A431 and HACAT cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab186421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 13 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionChemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.
Tissue specificityTestis, thymus, placenta, ovary and skin.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
- Information by UniProt
- ALP antibody
- C-C motif chemokine 27 antibody
- CC chemokine ILC antibody
Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling CCL27 with ab186421 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi.
Anti-CCL27 antibody [EPR8582(2)] (ab186421) at 1/5000 dilution + Human CCL27 recombinant protein at 0.01 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 13 kDa
Flow cytometric analysis of 2% paraformaldehyde-fixed HACAT cells labeling CCL27 with ab186421 at 1/320 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
ab186421 has been referenced in 1 publication.
- Li S et al. HOXC10 promotes proliferation and invasion and induces immunosuppressive gene expression in glioma. FEBS J 285:2278-2291 (2018). PubMed: 29676849