Recombinant Anti-CCL27 antibody [EPR8583(2)] (ab194354)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8583(2)] to CCL27
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-CCL27 antibody [EPR8583(2)]
See all CCL27 primary antibodies -
Description
Rabbit monoclonal [EPR8583(2)] to CCL27 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CCL27 aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: Q9Y4X3 -
Positive control
- Human CCL27 Recombinant protein; A431 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8583(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab194354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa). | |
| ICC/IF | 1/50. |
Target
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Function
Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10. -
Tissue specificity
Testis, thymus, placenta, ovary and skin. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 10850 Human
- Omim: 604833 Human
- SwissProt: Q9Y4X3 Human
- Unigene: 648124 Human
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Alternative names
- ALP antibody
- C-C motif chemokine 27 antibody
- CC chemokine ILC antibody
see all
Images
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![Western blot - Anti-CCL27 antibody [EPR8583(2)] (ab194354)](https://www.abcam.com/ps/products/194/ab194354/Images/ab194354-232168-ab194354-wb-1.jpg)
Western blot - Anti-CCL27 antibody [EPR8583(2)] (ab194354)Anti-CCL27 antibody [EPR8583(2)] (ab194354) at 1/20000 dilution + Human CCL27 Recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 13 kDa
Observed band size: 13 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8583(2)] (ab194354)](https://www.abcam.com/ps/products/194/ab194354/Images/ab194354-232167-ab194354IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8583(2)] (ab194354)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A431 cells labeling CCL27 with ab194354 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab194354 has not yet been referenced specifically in any publications.
