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RecombinantRabMAb

Recombinant Anti-CCL27 antibody [EPR8583(2)] (ab194354)

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Western blot - Anti-CCL27 antibody [EPR8583(2)] (ab194354)
  • Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8583(2)] (ab194354)
  • Anti-CCL27 antibody [EPR8583(2)] (ab194354)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8583(2)] to CCL27
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-CCL27 antibody [EPR8583(2)]
    See all CCL27 primary antibodies

  • Description

    Rabbit monoclonal [EPR8583(2)] to CCL27

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human CCL27 Recombinant protein; A431 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR8583(2)

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab194354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
ICC/IF
1/50.
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
ICC/IF
1/50.

Target

  • Function

    Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.

  • Tissue specificity

    Testis, thymus, placenta, ovary and skin.

  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • ALP antibody
      • C-C motif chemokine 27 antibody
      • CC chemokine ILC antibody
      • Ccl27 antibody
      • CCL27_HUMAN antibody
      • Chemokine (C-C motif) ligand 27 antibody
      • CTACK antibody
      • CTAK antibody
      • Cutaneous T cell attracting chemokine antibody
      • Cutaneous T-cell-attracting chemokine antibody
      • ESkine antibody
      • IL 11 R alpha locus chemokine antibody
      • IL-11 R-alpha-locus chemokine antibody
      • ILC antibody
      • PESKY antibody
      • SCYA27 antibody
      • Skinkine antibody
      • Small inducible cytokine A27 antibody
      • small inducible cytokine subfamily A (Cys-Cys), member 27 antibody
      • Small-inducible cytokine A27 antibody
      see all

    Images

    • Western blot - Anti-CCL27 antibody [EPR8583(2)] (ab194354)
      Western blot - Anti-CCL27 antibody [EPR8583(2)] (ab194354)
      Anti-CCL27 antibody [EPR8583(2)] (ab194354) at 1/20000 dilution + Human CCL27 Recombinant protein at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 13 kDa
      Observed band size: 13 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8583(2)] (ab194354)
      Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8583(2)] (ab194354)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A431 cells labeling CCL27 with ab194354 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).

    • Anti-CCL27 antibody [EPR8583(2)] (ab194354)
      Anti-CCL27 antibody [EPR8583(2)] (ab194354)

    References (0)

    Publishing research using ab194354? Please let us know so that we can cite the reference in this datasheet.

    ab194354 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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