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    ccl27-antibody-epr85832-ab194354.pdf

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Immunology Innate Immunity Chemokines Beta Chemokines (CC)
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RecombinantRabMAb

Recombinant Anti-CCL27 antibody [EPR8583(2)] (ab194354)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-CCL27 antibody [EPR8583(2)] (ab194354)
  • Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8583(2)] (ab194354)
  • Anti-CCL27 antibody [EPR8583(2)] (ab194354)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8583(2)] to CCL27
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Primary
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Anti-SLC20A2/PIT2 antibody (ab238337)

View more associated products

Overview

  • Product name

    Anti-CCL27 antibody [EPR8583(2)]
    See all CCL27 primary antibodies
  • Description

    Rabbit monoclonal [EPR8583(2)] to CCL27
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human CCL27 Recombinant protein; A431 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8583(2)
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokines (CC)

Associated products

  • Alternative Versions

    • Anti-CCL27 antibody [EPR8583(2)] - BSA and Azide free (ab251188)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Recombinant human CCL27 protein (ab50060)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab194354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
ICC/IF
1/50.
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
ICC/IF
1/50.

Target

  • Function

    Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.
  • Tissue specificity

    Testis, thymus, placenta, ovary and skin.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q9Y4X3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10850 Human
    • Omim: 604833 Human
    • SwissProt: Q9Y4X3 Human
    • Unigene: 648124 Human
    • Alternative names

      • ALP antibody
      • C-C motif chemokine 27 antibody
      • CC chemokine ILC antibody
      • Ccl27 antibody
      • CCL27_HUMAN antibody
      • Chemokine (C-C motif) ligand 27 antibody
      • CTACK antibody
      • CTAK antibody
      • Cutaneous T cell attracting chemokine antibody
      • Cutaneous T-cell-attracting chemokine antibody
      • ESkine antibody
      • IL 11 R alpha locus chemokine antibody
      • IL-11 R-alpha-locus chemokine antibody
      • ILC antibody
      • PESKY antibody
      • SCYA27 antibody
      • Skinkine antibody
      • Small inducible cytokine A27 antibody
      • small inducible cytokine subfamily A (Cys-Cys), member 27 antibody
      • Small-inducible cytokine A27 antibody
      see all

    Images

    • Western blot - Anti-CCL27 antibody [EPR8583(2)] (ab194354)
      Western blot - Anti-CCL27 antibody [EPR8583(2)] (ab194354)
      Anti-CCL27 antibody [EPR8583(2)] (ab194354) at 1/20000 dilution + Human CCL27 Recombinant protein at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 13 kDa
      Observed band size: 13 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8583(2)] (ab194354)
      Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8583(2)] (ab194354)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A431 cells labeling CCL27 with ab194354 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).

    • Anti-CCL27 antibody [EPR8583(2)] (ab194354)
      Anti-CCL27 antibody [EPR8583(2)] (ab194354)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab194354? Please let us know so that we can cite the reference in this datasheet.

    ab194354 has not yet been referenced specifically in any publications.

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