Overview

  • Product name

    Anti-CCL27 antibody [EPR8583(2)]
    See all CCL27 primary antibodies
  • Description

    Rabbit monoclonal [EPR8583(2)] to CCL27
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CCL27 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Y4X3

  • Positive control

    • Human CCL27 Recombinant protein; A431 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8583(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab194354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
ICC/IF 1/50.

Target

  • Function

    Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.
  • Tissue specificity

    Testis, thymus, placenta, ovary and skin.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • ALP antibody
    • C-C motif chemokine 27 antibody
    • CC chemokine ILC antibody
    • Ccl27 antibody
    • CCL27_HUMAN antibody
    • Chemokine (C-C motif) ligand 27 antibody
    • CTACK antibody
    • CTAK antibody
    • Cutaneous T cell attracting chemokine antibody
    • Cutaneous T-cell-attracting chemokine antibody
    • ESkine antibody
    • IL 11 R alpha locus chemokine antibody
    • IL-11 R-alpha-locus chemokine antibody
    • ILC antibody
    • PESKY antibody
    • SCYA27 antibody
    • Skinkine antibody
    • Small inducible cytokine A27 antibody
    • small inducible cytokine subfamily A (Cys-Cys), member 27 antibody
    • Small-inducible cytokine A27 antibody
    see all

Images

  • Anti-CCL27 antibody [EPR8583(2)] (ab194354) at 1/20000 dilution + Human CCL27 Recombinant protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 13 kDa
    Observed band size: 13 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A431 cells labeling CCL27 with ab194354 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).

References

ab194354 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab194354.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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