Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8583(2)] to CCL27
- Suitable for: WB, ICC/IF
- Reacts with: Human
Product nameAnti-CCL27 antibody [EPR8583(2)]
See all CCL27 primary antibodies
DescriptionRabbit monoclonal [EPR8583(2)] to CCL27
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CCL27 aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: Q9Y4X3
- Human CCL27 Recombinant protein; A431 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab194354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).|
FunctionChemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.
Tissue specificityTestis, thymus, placenta, ovary and skin.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
- Information by UniProt
- ALP antibody
- C-C motif chemokine 27 antibody
- CC chemokine ILC antibody
Anti-CCL27 antibody [EPR8583(2)] (ab194354) at 1/20000 dilution + Human CCL27 Recombinant protein at 0.01 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 13 kDa
Observed band size: 13 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A431 cells labeling CCL27 with ab194354 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).
ab194354 has not yet been referenced specifically in any publications.