Recombinant Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)
![Flow Cytometry (Intracellular) - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)](https://www.abcam.com/ps/products/254/ab254371/Images/ab254371-2-anti-ccl4mip1-beta-antibody-epr2361040-flow-cytometry-thp1.jpg "Flow Cytometry (Intracellular) - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23610-40] to CCL4/MIP-1 beta
- Suitable for: ICC, Flow Cyt (Intra), Dot blot
- Reacts with: Mouse, Human
Overview
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Product name
Anti-CCL4/MIP-1 beta antibody [EPR23610-40]
See all CCL4/MIP-1 beta primary antibodies -
Description
Rabbit monoclonal [EPR23610-40] to CCL4/MIP-1 beta -
Host species
Rabbit -
Specificity
This antibody has cross-reactivity with CCL4L1. There is about 97% homology between CCL4/MIP-1 beta and CCL4L1 in terms of the immunogen designed from CCL4/MIP-1 beta.
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Tested applications
Suitable for: ICC, Flow Cyt (Intra), Dot blotmore details
Unsuitable for: IHC-P,IP or WB -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC: THP-1 cells (treated with PMA, 100nM for 16 hours, then with lipopolysaccharide (LPS, 100 ng/ml) for 4 hours and with BFA (1 µg/ml) for another 3 hours.). Flow Cyt (intra): THP-1 (treated with 100nM phorbol 12-myristate 13-acetate (PMA) for 16 hours, then 100 ng/ml lipopolysaccharide (LPS) for 4 hours and add 1 ug/ml Brefeldin A (BFA) for another 3 hours) and RAW 264.7 cells (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours and 1 ug/ml Brefeldin A (BFA) for another 3 hours).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23610-40 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
1/100.
|
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| Flow Cyt (Intra) |
1/50.
|
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| Dot blot |
1/1000.
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| Notes |
|---|
|
ICC
1/100. |
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Flow Cyt (Intra)
1/50. |
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Dot blot
1/1000. |
Target
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Function
Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsN-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 388372 Human
- Entrez Gene: 6351 Human
- Entrez Gene: 9560 Human
- Entrez Gene: 20303 Mouse
- Omim: 182284 Human
- Omim: 603782 Human
- Omim: 610757 Human
- SwissProt: P13236 Human
see all -
Alternative names
- MIP 1 beta antibody
- Secreted protein G 26 antibody
- ACT 2 antibody
see all
Images
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Flow Cytometry (Intracellular) - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte) (treated with 100nM phorbol 12-myristate 13-acetate (PMA) for 16 hours, then 100 ng/ml lipopolysaccharide (LPS) for 4 hours and add 1 ug/ml Brefeldin A (BFA) for another 3 hours) (Red)/Untreated control (Green) cells labelling CCL4/MIP-1 beta with ab254371 at 1/500 dilution (0.1ug)/ compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
The expression pattern is consistent with what is described in the literature (PMID:29669317).
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![Immunocytochemistry - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)](https://www.abcam.com/ps/products/254/ab254371/Images/ab254371-1-anti-ccl4mip1-beta-antibody-epr2361040-immunocytochemistry-thp1.jpg)
Immunocytochemistry - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 cells labelling CCL4/MIP-1 beta with ab254371 at 1/100 (5.45 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in THP-1 cells treated with phorbol 12-myristate 13-acetate (PMA, 100nM) for 16 hours, then with lipopolysaccharide (LPS, 100 ng/ml) for 4 hours and with BFA (1 ug/ml) for another 3 hours. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Dot Blot - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)](https://www.abcam.com/ps/products/254/ab254371/Images/ab254371-4-anti-ccl4mip1-beta-antibody-epr2361040-dotblot-human-ccl4mip1-beta-immunogen-recombinant-protein.jpg)
Dot Blot - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)Dot blot analysis of CCL4/MIP-1 beta using ab254371 at 1/1000 (0.545ug/ml) dilution followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20,000 dilution.
Lane 1: Human CCL4/MIP-1 beta immunogen recombinant protein
Lane 2: Human CCL4L1 recombinant protein
Lane 3: Bovine serum albumin (BSA)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 114 seconds
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![Flow Cytometry (Intracellular) - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)](https://www.abcam.com/ps/products/254/ab254371/Images/ab254371-3-anti-ccl4mip1-beta-antibody-epr2361040-flow-cytometry-raw-2647.jpg)
Flow Cytometry (Intracellular) - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours and 1 ug/ml Brefeldin A (BFA) for another 3 hours) (Red) /Untreated control (Green) cells labelling CCL4/MIP-1 beta with ab254371 at 1/50 dilution (1ug)/ compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
The expression pattern is consistent with what is described in the literature (PMID:32230927).
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![Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)](https://www.abcam.com/ps/products/254/ab254371/Images/ab254371-5-benefits-of-recombinant-antibodies.png)
Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab254371 has not yet been referenced specifically in any publications.
