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  1. Link

    ccl4mip-1-beta-antibody-epr23610-40-ab254371.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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RecombinantRabMAb

Recombinant Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)

  • Datasheet
  • Certificate of Compliance
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Flow Cytometry (Intracellular) - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)
  • Immunocytochemistry - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)
  • Dot Blot - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)
  • Flow Cytometry (Intracellular) - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)
  • Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23610-40] to CCL4/MIP-1 beta
  • Suitable for: ICC, Flow Cyt (Intra), Dot blot
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-CCL4/MIP-1 beta antibody [EPR23610-40]
    See all CCL4/MIP-1 beta primary antibodies
  • Description

    Rabbit monoclonal [EPR23610-40] to CCL4/MIP-1 beta
  • Host species

    Rabbit
  • Specificity

    This antibody has cross-reactivity with CCL4L1. There is about 97% homology between CCL4/MIP-1 beta and CCL4L1 in terms of the immunogen designed from CCL4/MIP-1 beta.

  • Tested applications

    Suitable for: ICC, Flow Cyt (Intra), Dot blotmore details
    Unsuitable for: IHC-P,IP or WB
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: THP-1 cells (treated with PMA, 100nM for 16 hours, then with lipopolysaccharide (LPS, 100 ng/ml) for 4 hours and with BFA (1 µg/ml) for another 3 hours.). Flow Cyt (intra): THP-1 (treated with 100nM phorbol 12-myristate 13-acetate (PMA) for 16 hours, then 100 ng/ml lipopolysaccharide (LPS) for 4 hours and add 1 ug/ml Brefeldin A (BFA) for another 3 hours) and RAW 264.7 cells (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours and 1 ug/ml Brefeldin A (BFA) for another 3 hours).
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR23610-40
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokines (CC)
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV-related
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] - BSA and Azide free (ab275399)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab254371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
1/100.
Flow Cyt (Intra)
1/50.
Dot blot
1/1000.
Notes
ICC
1/100.
Flow Cyt (Intra)
1/50.
Dot blot
1/1000.
Application notes
Is unsuitable for IHC-P,IP or WB.

Target

  • Function

    Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications

    N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P13236 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 388372 Human
    • Entrez Gene: 6351 Human
    • Entrez Gene: 9560 Human
    • Entrez Gene: 20303 Mouse
    • Omim: 182284 Human
    • Omim: 603782 Human
    • Omim: 610757 Human
    • SwissProt: P13236 Human
    • SwissProt: Q8NHW4 Human
    • SwissProt: P14097 Mouse
    • Unigene: 655293 Human
    • Unigene: 661942 Human
    • Unigene: 75703 Human
    see all
  • Alternative names

    • MIP 1 beta antibody
    • Secreted protein G 26 antibody
    • ACT 2 antibody
    • ACT-2 antibody
    • ACT2 antibody
    • AT744.1 antibody
    • AT744.2 antibody
    • C C motif chemokine 4 antibody
    • C C motif chemokine 4 like antibody
    • C C motif chemokine ligand 4 like 1 antibody
    • C C motif chemokine ligand 4 like 2 antibody
    • CC chemokine ligand 4 antibody
    • CC chemokine ligand 4L1 antibody
    • CC chemokine ligand 4L1d2 antibody
    • CC chemokine ligand 4L2 antibody
    • CCL4 antibody
    • CCL4_HUMAN antibody
    • ccl4l 1 antibody
    • CCL4L antibody
    • CCL4L1 antibody
    • Chemokine (C C motif) ligand 4 antibody
    • Chemokine (C C motif) ligand 4 like 1 antibody
    • Chemokine (C C motif) ligand 4 like 1, telomeric antibody
    • Chemokine (C C motif) ligand 4 like 2 antibody
    • Chemokine CC Motif Ligand 4 antibody
    • G 26 antibody
    • G 26 T lymphocyte secreted protein antibody
    • G-26 T-lymphocyte-secreted protein antibody
    • HC21 antibody
    • Immune activation 2 antibody
    • LAG 1 antibody
    • LAG-1 antibody
    • LAG1 antibody
    • Lymphocyte activation gene 1 antibody
    • Lymphocyte activation gene 1 protein antibody
    • Macrophage inflammatory protein 1 beta antibody
    • Macrophage inflammatory protein 1-beta antibody
    • Macrophage inflammatory protein 1b2 antibody
    • MGC104418 antibody
    • MGC126025 antibody
    • MGC126026 antibody
    • MIP-1-beta antibody
    • MIP-1-beta(1-69) antibody
    • MIP-1-beta(3-69) antibody
    • MIP1 beta antibody
    • MIP1B antibody
    • MIP1B1 antibody
    • Monocyte adherence induced protein 5 alpha antibody
    • PAT 744 antibody
    • Protein H400 antibody
    • SCYA2 antibody
    • SCYA4 antibody
    • SCYA4L antibody
    • SCYA4L1 antibody
    • SCYA4L2 antibody
    • SCYQ4L2 antibody
    • Secreted protein G 26 antibody
    • Secreted protein G26 antibody
    • SIS gamma antibody
    • SIS-gamma antibody
    • Small inducible cytokine A4 (homologous to mouse Mip 1b) antibody
    • Small inducible cytokine A4 antibody
    • small inducible cytokine A4-like antibody
    • Small-inducible cytokine A4 antibody
    • T cell activation protein 2 antibody
    • T-cell activation protein 2 antibody
    see all

Images

  • Flow Cytometry (Intracellular) - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)
    Flow Cytometry (Intracellular) - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte) (treated with 100nM phorbol 12-myristate 13-acetate (PMA) for 16 hours, then 100 ng/ml lipopolysaccharide (LPS) for 4 hours and add 1 ug/ml Brefeldin A (BFA) for another 3 hours) (Red)/Untreated control (Green) cells labelling CCL4/MIP-1 beta with ab254371 at 1/500 dilution (0.1ug)/ compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). 

    A Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

    The expression pattern is consistent with what is described in the literature (PMID:29669317). 

     

     

  • Immunocytochemistry - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)
    Immunocytochemistry - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 cells labelling CCL4/MIP-1 beta with ab254371 at 1/100 (5.45 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in THP-1 cells treated with phorbol 12-myristate 13-acetate (PMA, 100nM) for 16 hours, then with lipopolysaccharide (LPS, 100 ng/ml) for 4 hours and with BFA (1 ug/ml) for another 3 hours. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Dot Blot - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)
    Dot Blot - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] (ab254371)

    Dot blot analysis of CCL4/MIP-1 beta using ab254371 at 1/1000 (0.545ug/ml) dilution followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20,000 dilution.

    Lane 1: Human CCL4/MIP-1 beta immunogen recombinant protein

    Lane 2: Human CCL4L1 recombinant protein

    Lane 3:  Bovine serum albumin (BSA)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 114 seconds

  • Flow Cytometry (Intracellular) - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)
    Flow Cytometry (Intracellular) - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours and 1 ug/ml Brefeldin A (BFA) for another 3 hours) (Red) /Untreated control (Green) cells labelling CCL4/MIP-1 beta with ab254371 at 1/50 dilution (1ug)/ compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). 

    A Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

    The expression pattern is consistent with what is described in the literature (PMID:32230927). 

     

     

  • Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)
    Anti-CCL4/MIP-1 beta antibody [EPR23610-40] (ab254371)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab254371? Please let us know so that we can cite the reference in this datasheet.

ab254371 has not yet been referenced specifically in any publications.

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