Recombinant
RabMAb

Recombinant Anti-CCL4/MIP-1 beta antibody [EPR494] - BSA and Azide free (ab232158)

Overview

  • Product name

    Anti-CCL4/MIP-1 beta antibody [EPR494] - BSA and Azide free
    See all CCL4/MIP-1 beta primary antibodies
  • Description

    Rabbit monoclonal [EPR494] to CCL4/MIP-1 beta - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CCL4/MIP-1 beta aa 50 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P13236

  • Positive control

    • ICC/IF: THP-1 cells.
  • General notes

    ab232158 is the carrier-free version of ab40857 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab232158 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Macrophage Inflammatory Protein 1 beta, MIP1 beta

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa).
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function

    Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications

    N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • MIP 1 beta antibody
    • Secreted protein G 26 antibody
    • ACT 2 antibody
    • ACT-2 antibody
    • ACT2 antibody
    • AT744.1 antibody
    • AT744.2 antibody
    • C C motif chemokine 4 antibody
    • C C motif chemokine 4 like antibody
    • C C motif chemokine ligand 4 like 1 antibody
    • C C motif chemokine ligand 4 like 2 antibody
    • CC chemokine ligand 4 antibody
    • CC chemokine ligand 4L1 antibody
    • CC chemokine ligand 4L1d2 antibody
    • CC chemokine ligand 4L2 antibody
    • CCL4 antibody
    • CCL4_HUMAN antibody
    • ccl4l 1 antibody
    • CCL4L antibody
    • CCL4L1 antibody
    • Chemokine (C C motif) ligand 4 antibody
    • Chemokine (C C motif) ligand 4 like 1 antibody
    • Chemokine (C C motif) ligand 4 like 1, telomeric antibody
    • Chemokine (C C motif) ligand 4 like 2 antibody
    • Chemokine CC Motif Ligand 4 antibody
    • G 26 antibody
    • G 26 T lymphocyte secreted protein antibody
    • G-26 T-lymphocyte-secreted protein antibody
    • HC21 antibody
    • Immune activation 2 antibody
    • LAG 1 antibody
    • LAG-1 antibody
    • LAG1 antibody
    • Lymphocyte activation gene 1 antibody
    • Lymphocyte activation gene 1 protein antibody
    • Macrophage inflammatory protein 1 beta antibody
    • Macrophage inflammatory protein 1-beta antibody
    • Macrophage inflammatory protein 1b2 antibody
    • MGC104418 antibody
    • MGC126025 antibody
    • MGC126026 antibody
    • MIP-1-beta antibody
    • MIP-1-beta(1-69) antibody
    • MIP-1-beta(3-69) antibody
    • MIP1 beta antibody
    • MIP1B antibody
    • MIP1B1 antibody
    • Monocyte adherence induced protein 5 alpha antibody
    • PAT 744 antibody
    • Protein H400 antibody
    • SCYA2 antibody
    • SCYA4 antibody
    • SCYA4L antibody
    • SCYA4L1 antibody
    • SCYA4L2 antibody
    • SCYQ4L2 antibody
    • Secreted protein G 26 antibody
    • Secreted protein G26 antibody
    • SIS gamma antibody
    • SIS-gamma antibody
    • Small inducible cytokine A4 (homologous to mouse Mip 1b) antibody
    • Small inducible cytokine A4 antibody
    • small inducible cytokine A4-like antibody
    • Small-inducible cytokine A4 antibody
    • T cell activation protein 2 antibody
    • T-cell activation protein 2 antibody
    see all

Images

  • ab40857 at 1/50 immunoprecipitating CCL4/MIP-1 beta + CCL4L in THP-1 (Human monocytic leukemia cell line) whole cell lysate observed at 12 KDa (lanes 1 and 2).

    Lane 1 (input): THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1 μg/mL Brefeldin A was added for the last 3 hours whole cell lysate, 10μg

    Lane 2 (+): ab40857 + THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1μg/mL Brefeldin A was added for the last 3 hours whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40857 in THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1 μg/mL Brefeldin A was added for the last 3 hours whole cell lysate

    For western blotting: ab40857 at 1/1000 followed by VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40857).

  • Immunocytochemistry/Immunofluorescence analysis of THP-1 (Human monocytic leukemia cell line) labeling CCL4/MIP-1 beta + CCL4L with ab40857 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200. Nuclei were counterstained with DAPI (blue).

    The expression increased after treatment with Lipopolysaccharides (LPS), 100 ng/mL for 4 hours, followed by addition of Brefeldin A (1 μg/mL) for 3 hours.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40857).

References

ab232158 has not yet been referenced specifically in any publications.

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