Overview

  • Product name

  • Description

    Rabbit polyclonal to CCM2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein (His-tag) corresponding to Human CCM2 aa 1-444.
    Database link: Q9BSQ5

  • Positive control

    • ICC/IF: Human foreskin tissue
      WB: CCM2 overexpressed in C0S1 cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Endotoxin level: <0.1 EU/1µg of ab129386 (LAL)
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab129386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 49 kDa.
ICC/IF 1/200.

Target

  • Function

    May function as a scaffold protein for MAP2K3-MAP3K3 signaling. Seems to play a major role in the modulation of MAP3K3-dependent p38 activation induced by hyperosmotic shock.
  • Involvement in disease

    Defects in CCM2 are the cause of cerebral cavernous malformations type 2 (CCM2) [MIM:603284]. Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and are usually present clinically during the 3rd to 5th decades of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters.
  • Sequence similarities

    Contains 1 PID domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • C7orf22 antibody
    • Ccm2 antibody
    • CCM2 gene antibody
    • CCM2_HUMAN antibody
    • Cerebral cavernous malformation 2 antibody
    • Cerebral cavernous malformations 2 protein antibody
    • Chromosome 7 open reading frame 22 antibody
    • Malcavernin antibody
    • MGC4067 antibody
    • MGC4607 antibody
    • MGC74868 antibody
    • OSM antibody
    • Osmosensing scaffold for MEKK3 antibody
    • OTTHUMP00000159554 antibody
    • OTTHUMP00000214270 antibody
    • OTTHUMP00000214271 antibody
    • OTTHUMP00000214273 antibody
    • PP10187 antibody
    see all

Images

  • All lanes : Anti-CCM2 antibody (ab129386) at 5 µg/ml

    Lane 1 : Mwt marker
    Lane 2 : CCM2 knockout
    Lane 3 : CCM2 wildtype
    Lane 4 : CCM2 overexpressed in C0S1 cells

    Predicted band size: 49 kDa

  • All lanes : Anti-CCM2 antibody (ab129386) at 5 µg/ml

    Lane 1 : Mwt marker
    Lane 2 : CCM2 knockout
    Lane 3 : CCM2 wildtype
    Lane 4 : CCM2 overexpressed in C0S1 cells

    Predicted band size: 49 kDa

  • ab129386, at a 1/50 dilution, staining CCM2 (green) in a cryo-section of unfixed Human foreskin tissue by Immunofluorescence.
    A) Note specific staining in the wall of microvessels.
    B) Negative control of a consecutive section.
    Note non-specific fluorescence in elastic fibres in the adventitia of an arteriol.
    Nuclei counter-stained with Dapi (blue).

References

ab129386 has not yet been referenced specifically in any publications.

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