Key features and details
- Mouse monoclonal [OTI2E4] to CCM2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CCM2 antibody [OTI2E4]
See all CCM2 primary antibodies
DescriptionMouse monoclonal [OTI2E4] to CCM2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human CCM2 aa 1-444. Produced in HEK-293T cells (NP_113631).
MEEEGKKGKKPGIVSPFKRVFLKGEKSRDKKAHEKVTERRPLHTVVLSLP ERVEPDRLLSDYIEKEVKYLGQLTSIPGYLNPSSRTEILHFIDNAKRAHQ LPGHLTQEHDAVLSLSAYNVKLAWRDGEDIILRVPIHDIAAVSYVRDDAA HLVVLKTAQDPGISPSQSLCAESSRGLSAGSLSESAVGPVEACCLVILAA ESKVAAEELCCLLGQVFQVVYTESTIDFLDRAIFDGASTPTHHLSLHSDD SSTKVDIKETYEVEASTFCFPESVDVGGASPHSKTISESELSASATELLQ DYMLTLRTKLSSQEIQQFAALLHEYRNGASIHEFCINLRQLYGDSRKFLL LGLRPFIPEKDSQHFENFLETIGVKDGRGIITDSFGRHRRALSTTSSSTT NGNRATGSSDDRSAPSEGDEWDRMISDISSDIEALGCSMDQDSA
Database link: Q9BSQ5
- WB: HEK-293T cells transfected with pCMV6-ENTRY CCM2 cDNA. IHC-P: Human kidney tissue. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY CCM2 cDNA.
Clone OTI2E4 (formerly 2E4).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Purification notesPurified from cell culture supernatant by affinity chromatography.
Our Abpromise guarantee covers the use of ab123930 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 49 kDa.|
|IHC-P||1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionMay function as a scaffold protein for MAP2K3-MAP3K3 signaling. Seems to play a major role in the modulation of MAP3K3-dependent p38 activation induced by hyperosmotic shock.
Involvement in diseaseDefects in CCM2 are the cause of cerebral cavernous malformations type 2 (CCM2) [MIM:603284]. Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and are usually present clinically during the 3rd to 5th decades of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters.
Sequence similaritiesContains 1 PID domain.
- Information by UniProt
- C7orf22 antibody
- Ccm2 antibody
- CCM2 gene antibody
All lanes : Anti-CCM2 antibody [OTI2E4] (ab123930) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY CCM2 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 49 kDa
pCMV6-ENTRY CCM2 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for CCM2 using ab123930 at a 1/100 dilution in ICC/IF.
Paraffin-embedded human kidney tissue stained for CCM2 with ab123930 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab123930 has been referenced in 1 publication.
- Costa B et al. STK25 Protein Mediates TrkA and CCM2 Protein-dependent Death in Pediatric Tumor Cells of Neural Origin. J Biol Chem 287:29285-9 (2012). PubMed: 22782892