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Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human CCN1.
(Peptide available as ab24416.)
Our Abpromise guarantee covers the use of ab10760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab10760 staining CCN1 in mouse kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded). Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in Tris-EDTA buffer pH9.0 and bloking for 15 minutes at 20°C (5 minutes/peroxidase block and 10 minutes/protein block). The primary antibody was diluted 1/100 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used, undiluted as the secondary.
Western blot for CCN1 antibody (ab10760) at 1/500 tested on the following human tissue lysates:
Lane 1: hela nuclear
Lane 2: hela
Lane 3: A431
Lane 4: Jurkat
Secondary ab: Alexa Fluor Goat polyclonal to Rabbit IgG (700) [1/5000]
Expected molecular weight: 42 kDa Observed molecular weight: ~50 kDa
ab10760 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"