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Synthetic peptide corresponding to Mouse CCN1. Synthetic peptide surrounding amino acid 166 of mouse Cyr61. The peptide was 10-15 amino acids long.
(Peptide available as
Our Abpromise guarantee covers the use of ab24448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50. PubMed: 19077055|
|IHC-FoFr||Use at an assay dependent concentration. PMID 17724216|
|IHC-P||Use a concentration of 2 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21490242|
|WB||Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 42 kDa).Can be blocked with CCN1 peptide (ab52125).|
Immunocytochemistry/ Immunofluorescence analysis of human osteosarcoma cells labeling CCN1 with ab24448 at 1/100 dilution. Cells were fixed with paraformaldehyde and blocked with 0.05% triton-X100. The cells were blocked with 3% BSA for 1 hour at 20°C followed by incubation with ab24448 in TBS/Tween-20 + 1% BSA for 2 hours at 20°C. A Donkey anti-rabbit Cy3® secondary antibody was used at 1/200 dilution.
Immunocytochemistry/ Immunofluorescence analysis of human synovial fibroblasts labeling CCN1 with ab24448 at 1/200 dilution. Cells were fixed with paraformaldehyde and permeabilized with methanol. The cells were blocked with 1% BSA for 1 hour at 25°C. The cells were incubated with PBS with ab24448 in 1% BSA for 2 hours at 25°C. A goat anti-rabbit Cy5® secondary antibody was used.
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