Overview

  • Product name
  • Description
    Rabbit polyclonal to CCN3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 58-357 of Human CCN3 (Uniprot ID: P48745).

  • Positive control
    • Recombinant Human CCN3 protein (ab50076) can be used as a positive control in WB. 293T, A431, H1299, HeLaS2, Hela, HepG2, MOLT4, Mouse brain and Raji whole cell lysates; HeLa cells; Human gastric carcinoma N87 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0)
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Immediate-early protein likely to play a role in cell growth regulation.
  • Tissue specificity
    Expressed in bone marrow, thymic cells and nephroblastoma. Increased expression in Wilms tumor of the stromal type.
  • Sequence similarities
    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCN family member 3 antibody
    • CCN3 antibody
    • IBP-9 antibody
    • IGF-binding protein 9 antibody
    • IGFBP-9 antibody
    • IGFBP9 antibody
    • Insulin-like growth factor-binding protein 9 antibody
    • Nephroblastoma overexpressed antibody
    • Nephroblastoma overexpressed gene protein homolog antibody
    • Nephroblastoma-overexpressed gene protein homolog antibody
    • NOV antibody
    • NOV_HUMAN antibody
    • NovH antibody
    • Protein NOV homolog antibody
    see all

Images

  • Anti-CCN3 antibody (ab137677) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 39 kDa



    12% SDS PAGE
  • Anti-CCN3 antibody (ab137677) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 39 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling CCN3 using ab137677 at 1/200 dilution. Lower image is merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma N87 xenograft tissue, labelling CCN3 with ab137677 at 1/100 dilution.

References

This product has been referenced in:
  • Liu HF  et al. CCN3 suppresses TGF-ß1-induced extracellular matrix accumulation in human mesangial cells in vitro. Acta Pharmacol Sin 39:222-229 (2018). Read more (PubMed: 28858296) »
  • Li J  et al. Reduced NOV expression correlates with disease progression in colorectal cancer and is associated with survival, invasion and chemoresistance of cancer cells. Oncotarget 8:26231-26244 (2017). Read more (PubMed: 28412738) »
See all 4 Publications for this product

Customer reviews and Q&As

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Application
Western blot
Sample
Mouse Tissue lysate - whole (3T3L1 AND ADIPOSE TISSUE)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
50 µg
Specification
3T3L1 AND ADIPOSE TISSUE
Blocking step
tbs as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 30°C

Dr. Shailendra Singh

Verified customer

Submitted Aug 15 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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