RecombinantRabMAb

Recombinant Anti-CCN3 antibody [EPR8781(3)] (ab191425)

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Western blot - Anti-CCN3 antibody [EPR8781(3)] (ab191425)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCN3 antibody [EPR8781(3)] (ab191425)
  • Western blot - Anti-CCN3 antibody [EPR8781(3)] (ab191425)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCN3 antibody [EPR8781(3)] (ab191425)
  • Anti-CCN3 antibody [EPR8781(3)] (ab191425)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8781(3)] to CCN3
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CCN3 antibody [EPR8781(3)]
    See all CCN3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8781(3)] to CCN3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, 293, Caco-2 and TT lysates; Human hepatocellular carcinoma and Human pancreas tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR8781(3)

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab191425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 39 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 39 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Immediate-early protein likely to play a role in cell growth regulation.

  • Tissue specificity

    Expressed in bone marrow, thymic cells and nephroblastoma. Increased expression in Wilms tumor of the stromal type.

  • Sequence similarities

    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CCN family member 3 antibody
    • CCN3 antibody
    • IBP-9 antibody
    • IGF-binding protein 9 antibody
    • IGFBP-9 antibody
    • IGFBP9 antibody
    • Insulin-like growth factor-binding protein 9 antibody
    • Nephroblastoma overexpressed antibody
    • Nephroblastoma overexpressed gene protein homolog antibody
    • Nephroblastoma-overexpressed gene protein homolog antibody
    • NOV antibody
    • NOV_HUMAN antibody
    • NovH antibody
    • Protein NOV homolog antibody
    see all

Images

  • Western blot - Anti-CCN3 antibody [EPR8781(3)] (ab191425)
    Western blot - Anti-CCN3 antibody [EPR8781(3)] (ab191425)
    All lanes : Anti-CCN3 antibody [EPR8781(3)] (ab191425) at 1/10000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : 293 lysate
    Lane 3 : Caco-2 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCN3 antibody [EPR8781(3)] (ab191425)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCN3 antibody [EPR8781(3)] (ab191425)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CCN3 with ab191425 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin. Inset: Negative control: using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-CCN3 antibody [EPR8781(3)] (ab191425)
    Western blot - Anti-CCN3 antibody [EPR8781(3)] (ab191425)
    Anti-CCN3 antibody [EPR8781(3)] (ab191425) at 1/5000 dilution + TT lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCN3 antibody [EPR8781(3)] (ab191425)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCN3 antibody [EPR8781(3)] (ab191425)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling CCN3 with ab191425 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.  Inset: Negative control: using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-CCN3 antibody [EPR8781(3)] (ab191425)
    Anti-CCN3 antibody [EPR8781(3)] (ab191425)

References (1)

Publishing research using ab191425? Please let us know so that we can cite the reference in this datasheet.

ab191425 has been referenced in 1 publication.

  • Zhu HP  et al. Regulatory mechanism of NOV/CCN3 in the inflammation and apoptosis of lung epithelial alveolar cells upon lipopolysaccharide stimulation. Mol Med Rep 21:1872-1880 (2020). PubMed: 31545412

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