Key features and details
- Rabbit polyclonal to CCNO
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-CCNO antibody
See all CCNO primary antibodies
DescriptionRabbit polyclonal to CCNO
SpecificityAt least two isoforms of Cyclin O are known to exist; this antibody will recognize both isoforms.
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
A 14 amino acid peptide from near the amino terminus of human Cyclin O(Genbank accession No. NP_066970).
- Mouse bladder tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab47682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the cyclin family.
Developmental stageMaximum levels during G(1) phase. Levels decrease through S and G(2) phases.
- Information by UniProt
- CCNO antibody
- CCNO_HUMAN antibody
- CCNU antibody
Western blot analysis of in mouse bladder tissue lysate with ab47682 antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml. Western blot analysis of ab47682 in mouse bladder tissue lysate with ab47682 antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml.
ICC/IF image of ab47682 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47682, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab47682 staining in human normal breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47682, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab47682 has been referenced in 4 publications.
- Sánchez-Botet A et al. The atypical cyclin CNTD2 promotes colon cancer cell proliferation and migration. Sci Rep 8:11797 (2018). PubMed: 30087414
- Li L et al. Knockdown of CCNO decreases the tumorigenicity of gastric cancer by inducing apoptosis. Onco Targets Ther 11:7471-7481 (2018). PubMed: 30464498
- Gasa L et al. A systematic analysis of orphan cyclins reveals CNTD2 as a new oncogenic driver in lung cancer. Sci Rep 7:10228 (2017). PubMed: 28860486
- Orthwein A et al. Regulation of activation-induced deaminase stability and antibody gene diversification by Hsp90. J Exp Med 207:2751-65 (2010). WB ; Human . PubMed: 21041454