Question (41895) | Anti-CCNYL1 antibody (ab87161)

Go to datasheet (ab87161)

Question

We have encountered problems with ab87161. Please check the attachment for
the details.

Answer

Thank you for sending your protocol and the image of your blot. You have already tried most of the protocol modifications I would recommend in this case. The only other suggestion I would make is that it might help to use 5% milk for both the blocking buffer and antibody diluents. You may also find that the background signal is reduced by increasing the blocking time to 2-3 hours or overnight at 4C.

After reviewing the image you sent, it is possible that the band around 45 kDa may correspond to isoform 1 of CCNYL1: http://www.uniprot.org/uniprot/Q8N7R7

If you are unsatisfied with your results I would be more than happy to offer you a credit or a refund, but unfortunately we do not have a different antibody to this target at this time. Please let me know your original order number and how you would like to proceed and I will be happy to assist you further.

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