Overview

  • Product name

  • Description

    Rabbit polyclonal to CCR1
  • Host species

    Rabbit
  • Specificity

    ab19013 recognises CCR1
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human CCR1. Surrounding amino acid 341
    (Peptide available as ab185720)

  • Positive control

    • A357 cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Thimerosal (merthiolate)
    Constituents: PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 43 kDa.Can be blocked with CCR1 peptide (ab185720).

Target

  • Function

    Receptor for a C-C type chemokine. Binds to MIP-1-alpha, MIP-1-delta, RANTES, and MCP-3 and, less efficiently, to MIP-1-beta or MCP-1 and subsequently transduces a signal by increasing the intracellular calcium ions level. Responsible for affecting stem cell proliferation.
  • Tissue specificity

    Widely expressed in different hematopoietic cells.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C C chemokine receptor type 1 antibody
    • C C CKR 1 antibody
    • C-C chemokine receptor type 1 antibody
    • C-C CKR-1 antibody
    • CC CKR 1 antibody
    • CC-CKR-1 antibody
    • CCR 1 antibody
    • CCR-1 antibody
    • CCR1 antibody
    • CCR1_HUMAN antibody
    • CD 191 antibody
    • CD191 antibody
    • CD191 antigen antibody
    • Chemokine (C C motif) receptor 1 antibody
    • Chemokine C C motif receptor 1 antibody
    • CKR 1 antibody
    • CKR1 antibody
    • CMKBR 1 antibody
    • CMKBR1 antibody
    • CMKR 1 antibody
    • CMKR1 antibody
    • HM145 antibody
    • LD78 receptor antibody
    • Macrophage inflammatory protein 1 alpha /Rantes receptor antibody
    • Macrophage inflammatory protein 1 alpha receptor antibody
    • Macrophage inflammatory protein 1-alpha receptor antibody
    • Mip 1a R antibody
    • MIP 1alpha R antibody
    • MIP-1alpha-R antibody
    • MIP1aR antibody
    • RANTES R antibody
    • RANTES receptor antibody
    • RANTES-R antibody
    • SCYAR1 antibody
    see all

Images

  • Anti-CCR1 antibody (ab19013) at 1 µg/ml + A357 cell lysate

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

References

This product has been referenced in:

  • Pincha N  et al. PAI1 mediates fibroblast-mast cell interactions in skin fibrosis. J Clin Invest 128:1807-1819 (2018). Read more (PubMed: 29584619) »
  • Zhang Y  et al. Effect of Tetramethylpyrazine on Atherosclerosis and SCAP/SREBP-1c Signaling Pathway in ApoE(-/-) Mice Fed with a High-Fat Diet. Evid Based Complement Alternat Med 2017:3121989 (2017). Read more (PubMed: 28491104) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us. Since the antibody was raised against the full length protein (Recombinant full length protein (Human): CCR1), and epitope mapping has not been performed - we do not have information about where the antibody binding site would be on the protein. This information is unfortunately unknown. I am very sorry to not be able to help in this case. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us for technical support and the protocol details, the image is very useful to understand the customer's problem. Is the customer using A357 cell lysate, not A375? This is a good positive control and ab19013 should work very well with it. The streaks on the lanes make me and my colleagues believe the problem is due to something gone off or missing in the migration process, it could be the gel, the buffers or the state of the samples too, especially as there are few protease inhibitors in the lysis buffer. Can I also confirm that the gel is reducing and the samples were reduced? What is the loading buffer recipe and gel recipe? Is there SDS in the running buffer? Finally, how long did the customer block the membrane for? Has the customer tried 5%BSA 1hr and incubating the antibody in TBST? If the customer would like our recommended WB tips and recipes please direct him/her to https://www.abcam.com/index.html?pageconfig=resource&rid=10413 I hope this information helps, please do not hesitate to contact me if I can be of further assistance,

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