Overview

  • Product name

    Anti-CCR2 antibody [EPR19698]
    See all CCR2 primary antibodies
  • Description

    Rabbit monoclonal [EPR19698] to CCR2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    This product was produced with the following immunogens:
    Recombinant fragment within Mouse CCR2 aa 1-100. The exact sequence is proprietary.
    Database link: P51683

    Recombinant fragment within Mouse CCR2 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: P51683

  • Positive control

    • WB: Mouse spleen and brain lysates; RAW 264.7 and NIH/3T3 whole cell lysates; J774A.1 whole cell lysates untreated and treated with 500U/ml IFN gamma for 4 hours. ICC/IF: RAW 264.7 and J774A.1 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab203128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • Function

    Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C C chemokine receptor type 2 antibody
    • C C CKR 2 antibody
    • C-C chemokine receptor type 2 antibody
    • C-C CKR-2 antibody
    • CC chemokine receptor type 2 antibody
    • CC CKR 2 antibody
    • CC-CKR-2 antibody
    • CCCKR2 antibody
    • CCR 2 antibody
    • CCR-2 antibody
    • CCR1L antibody
    • CCR2 antibody
    • CCR2_HUMAN antibody
    • CCR2A antibody
    • CCR2B antibody
    • CCR5L antibody
    • CD192 antibody
    • CD192 antigen antibody
    • Chemokine C C motif receptor 2 antibody
    • Chemokine CC Motif Receptor 2 antibody
    • CKR 2 antibody
    • CKR2 antibody
    • CKR2A antibody
    • CKR2B antibody
    • CMKBR2 antibody
    • MCP 1 R antibody
    • MCP-1-R antibody
    • MCP1 RECEPTOR antibody
    • MCP1R antibody
    • Monocyte chemoattractant protein 1 receptor antibody
    • Monocyte Chemotactic Protein 1 Receptor antibody
    • Monocyte Chemotactic Protein 1 Receptor antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) cells labeling CCR2 with ab203128 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on J774A.1 cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

  • All lanes : Anti-CCR2 antibody [EPR19698] (ab203128) at 1/1000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling CCR2 with ab203128 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on RAW 264.7 cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

  • All lanes : Anti-CCR2 antibody [EPR19698] (ab203128) at 1/5000 dilution

    Lane 1 : Untreated J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate
    Lane 2 : J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) treated with 500U/ml IFN gamma for 4 hours whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Pro-inflammatory cytokines such as IFN-gamma, IL-1, or TNF treatment decreases CCR2 expression (PMID: 9558092).

References

This product has been referenced in:

  • Kazakevych J  et al. Transcriptome analysis identifies a robust gene expression program in the mouse intestinal epithelium on aging. Sci Rep 9:10410 (2019). Read more (PubMed: 31320724) »
  • Biguetti CC  et al. CCR2 Contributes to F4/80+ Cells Migration Along Intramembranous Bone Healing in Maxilla, but Its Deficiency Does Not Critically Affect the Healing Outcome. Front Immunol 9:1804 (2018). Read more (PubMed: 30147688) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue (infiltrating mouse brain monocytes and mouse bone)
Permeabilization
Yes - 0.3% triton in PBS
Specification
infiltrating mouse brain monocytes and mouse bone
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 25°C
Fixative
Paraformaldehyde

Mr. Amanda Sierra

Verified customer

Submitted Oct 07 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Small intestine)
Permeabilization
Yes - 0.3% TritonX/PBS
Specification
Small intestine
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Apr 05 2019

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