Recombinant Anti-CCR2 antibody [EPR19698] (ab203128)
![Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR19698] (ab203128)](https://www.abcam.com/ps/products/203/ab203128/Images/ab203128-266590-anti-ccr2-antibody-epr19698-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR19698] (ab203128)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19698] to CCR2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
Overview
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Product name
Anti-CCR2 antibody [EPR19698]
See all CCR2 primary antibodies -
Description
Rabbit monoclonal [EPR19698] to CCR2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
This product was produced with the following immunogens:
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Recombinant fragment within Mouse CCR2 aa 300 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P51683 -
Positive control
- WB: Mouse spleen and brain lysates; RAW 264.7 and NIH/3T3 whole cell lysates; J774A.1 whole cell lysates untreated and treated with 500U/ml IFN gamma for 4 hours. ICC/IF: RAW 264.7 and J774A.1 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19698 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203128 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100.
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| WB |
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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| Notes |
|---|
|
ICC/IF
1/100. |
|
WB
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
Target
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Function
Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12772 Mouse
- SwissProt: P51683 Mouse
- Unigene: 6272 Mouse
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Alternative names
- C C chemokine receptor type 2 antibody
- C C CKR 2 antibody
- C-C chemokine receptor type 2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR19698] (ab203128)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) cells labeling CCR2 with ab203128 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on J774A.1 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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![Western blot - Anti-CCR2 antibody [EPR19698] (ab203128)](https://www.abcam.com/ps/products/203/ab203128/Images/ab203128-266593-anti-ccr2-antibody-epr19698-western-blot.jpg)
Western blot - Anti-CCR2 antibody [EPR19698] (ab203128)All lanes : Anti-CCR2 antibody [EPR19698] (ab203128) at 1/1000 dilution
Lane 1 : Mouse spleen lysate
Lane 2 : Mouse brain lysate
Lane 3 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR19698] (ab203128)](https://www.abcam.com/ps/products/203/ab203128/Images/ab203128-266591-anti-ccr2-antibody-epr19698-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR19698] (ab203128)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling CCR2 with ab203128 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on RAW 264.7 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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![Western blot - Anti-CCR2 antibody [EPR19698] (ab203128)](https://www.abcam.com/ps/products/203/ab203128/Images/ab203128-266592-anti-ccr2-antibody-epr19698-western-blot.jpg)
Western blot - Anti-CCR2 antibody [EPR19698] (ab203128)All lanes : Anti-CCR2 antibody [EPR19698] (ab203128) at 1/5000 dilution
Lane 1 : Untreated J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate
Lane 2 : J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) treated with 500U/ml IFN gamma for 4 hours whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Pro-inflammatory cytokines such as IFN-gamma, IL-1, or TNF treatment decreases CCR2 expression (PMID: 9558092).
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![Anti-CCR2 antibody [EPR19698] (ab203128)](https://www.abcam.com/ps/products/203/ab203128/Images/ab203128-6-benefits-of-recombinant-antibodies.png)
Anti-CCR2 antibody [EPR19698] (ab203128)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (13)
ab203128 has been referenced in 13 publications.
- Ou X et al. Activation of SIRT1 promotes cartilage differentiation and reduces apoptosis of nucleus pulposus mesenchymal stem cells via the MCP1/CCR2 axis in subjects with intervertebral disc degeneration. Int J Mol Med 46:1074-1084 (2020). PubMed: 32705163
- Zhang W et al. Ly-6Chigh inflammatory-monocyte recruitment is regulated by p38 MAPK/MCP-1 activation and promotes ventilator-induced lung injury. Int Immunopharmacol 78:106015 (2020). PubMed: 31780369
- Guo L et al. Proinflammatory macrophage-derived microvesicles exhibit tumor tropism dependent on CCL2/CCR2 signaling axis and promote drug delivery via SNARE-mediated membrane fusion. Theranostics 10:6581-6598 (2020). PubMed: 32550891
- Lu J et al. Regulatory T Cells as a Novel Candidate for Cell-Based Therapy in Kidney Disease. Front Physiol 11:621 (2020). PubMed: 32581852
- Han F et al. TL1A primed dendritic cells activation exacerbated chronic murine colitis. Life Sci 262:118220 (2020). PubMed: 32781075
