For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Explore the power of knock-out cell lines for your research

  1. Link

    ccr2-antibody-epr19698-ab203128.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Innate Immunity Macrophage / Inflamm.
Share by email
RecombinantRabMAb

Recombinant Anti-CCR2 antibody [EPR19698] (ab203128)

  • Datasheet
  • Certificate of Compliance
Reviews (2) Submit a question References (20)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR19698] (ab203128)
  • Western blot - Anti-CCR2 antibody [EPR19698] (ab203128)
  • Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR19698] (ab203128)
  • Western blot - Anti-CCR2 antibody [EPR19698] (ab203128)
  • Anti-CCR2 antibody [EPR19698] (ab203128)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19698] to CCR2
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

You may also be interested in

ELISA
Product image
Mouse IL-1 beta ELISA Kit (ab100704)
Protein
Product image
Recombinant Human CCR2 protein (ab114168)
Primary
Product image
Anti-CD64 antibody [EPR4624] (ab134073)

View more associated products

Overview

  • Product name

    Anti-CCR2 antibody [EPR19698]
    See all CCR2 primary antibodies
  • Description

    Rabbit monoclonal [EPR19698] to CCR2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    This product was produced with the following immunogens:
    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

    Recombinant fragment within Mouse CCR2 aa 300 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P51683

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Mouse spleen and brain lysates; RAW 264.7 and NIH/3T3 whole cell lysates; J774A.1 whole cell lysates untreated and treated with 500U/ml IFN gamma for 4 hours. ICC/IF: RAW 264.7 and J774A.1 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19698
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokine Rec. (CCR)
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cancer
    • Signal transduction
    • G protein signaling
    • GPCR
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Chemokines

Associated products

  • Alternative Versions

    • Anti-CCR2 antibody [EPR19698] - Low endotoxin, Azide free (ab222496)
    • Alexa Fluor® 647 Anti-CCR2 antibody [EPR19698] (ab225432)
    • Alexa Fluor® 488 Anti-CCR2 antibody [EPR19698] (ab225433)
    • Anti-CCR2 antibody [EPR19698] - BSA and Azide free (ab271969)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CCR2 protein (ab114168)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab203128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.
WB
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
Notes
ICC/IF
1/100.
WB
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • Function

    Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P41597 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 12772 Mouse
    • SwissProt: P51683 Mouse
    • Unigene: 6272 Mouse
    • Alternative names

      • C C chemokine receptor type 2 antibody
      • C C CKR 2 antibody
      • C-C chemokine receptor type 2 antibody
      • C-C CKR-2 antibody
      • CC chemokine receptor type 2 antibody
      • CC CKR 2 antibody
      • CC-CKR-2 antibody
      • CCCKR2 antibody
      • CCR 2 antibody
      • CCR-2 antibody
      • CCR1L antibody
      • CCR2 antibody
      • CCR2_HUMAN antibody
      • CCR2A antibody
      • CCR2B antibody
      • CCR5L antibody
      • CD192 antibody
      • CD192 antigen antibody
      • Chemokine C C motif receptor 2 antibody
      • Chemokine CC Motif Receptor 2 antibody
      • CKR 2 antibody
      • CKR2 antibody
      • CKR2A antibody
      • CKR2B antibody
      • CMKBR2 antibody
      • MCP 1 R antibody
      • MCP-1-R antibody
      • MCP1 RECEPTOR antibody
      • MCP1R antibody
      • Monocyte chemoattractant protein 1 receptor antibody
      • Monocyte Chemotactic Protein 1 Receptor antibody
      • Monocyte Chemotactic Protein 1 Receptor antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR19698] (ab203128)
      Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR19698] (ab203128)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) cells labeling CCR2 with ab203128 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing cytoplasmic staining on J774A.1 cell line.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

    • Western blot - Anti-CCR2 antibody [EPR19698] (ab203128)
      Western blot - Anti-CCR2 antibody [EPR19698] (ab203128)
      All lanes : Anti-CCR2 antibody [EPR19698] (ab203128) at 1/1000 dilution

      Lane 1 : Mouse spleen lysate
      Lane 2 : Mouse brain lysate
      Lane 3 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
      Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 43 kDa
      Observed band size: 43 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR19698] (ab203128)
      Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR19698] (ab203128)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling CCR2 with ab203128 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing cytoplasmic staining on RAW 264.7 cell line.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

    • Western blot - Anti-CCR2 antibody [EPR19698] (ab203128)
      Western blot - Anti-CCR2 antibody [EPR19698] (ab203128)
      All lanes : Anti-CCR2 antibody [EPR19698] (ab203128) at 1/5000 dilution

      Lane 1 : Untreated J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate
      Lane 2 : J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) treated with 500U/ml IFN gamma for 4 hours whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 43 kDa
      Observed band size: 43 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      Pro-inflammatory cytokines such as IFN-gamma, IL-1, or TNF treatment decreases CCR2 expression (PMID: 9558092).

    • Anti-CCR2 antibody [EPR19698] (ab203128)
      Anti-CCR2 antibody [EPR19698] (ab203128)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (20)

    Publishing research using ab203128? Please let us know so that we can cite the reference in this datasheet.

    ab203128 has been referenced in 20 publications.

    • Xu P  et al. Recruitment of ?d T cells to the lesion via the CCL2/CCR2 signaling after spinal cord injury. J Neuroinflammation 18:64 (2021). PubMed: 33653377
    • Bordoloi J  et al. Gamma-glutamyl carboxylated Gas6 facilitates the prophylactic effect of vitamin K in inhibiting hyperlipidemia-associated inflammatory pathophysiology via arresting MCP-1/ICAM-1 mediated monocyte-hepatocyte adhesion. J Nutr Biochem 93:108635 (2021). PubMed: 33789149
    • Tang RZ  et al. Sanjie Yiliu Formula Inhibits Colorectal Cancer Growth by Suppression of Proliferation and Induction of Apoptosis. ACS Omega 6:7761-7770 (2021). PubMed: 33778287
    • Wu T  et al. A modified laser-induced choroidal neovascularization animal model with intravitreal oxidized low-density lipoprotein. Int J Ophthalmol 13:1187-1194 (2020). PubMed: 32821671
    • Liu X  et al. Fruquintinib inhibits VEGF/VEGFR2 axis of choroidal endothelial cells and M1-type macrophages to protect against mouse laser-induced choroidal neovascularization. Cell Death Dis 11:1016 (2020). PubMed: 33247124
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    1-2 of 2 Abreviews

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CCR2 antibody [EPR19698]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue (infiltrating mouse brain monocytes and mouse bone)
    Permeabilization
    Yes - 0.3% triton in PBS
    Specification
    infiltrating mouse brain monocytes and mouse bone
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Amanda Sierra

    Verified customer

    Submitted Oct 07 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-CCR2 antibody [EPR19698]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Small intestine)
    Permeabilization
    Yes - 0.3% TritonX/PBS
    Specification
    Small intestine
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 05 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.