Recombinant
RabMAb

Recombinant Anti-CCR2 antibody [EPR19698] (Alexa Fluor® 647) (ab225432)

Overview

  • Product name

    Anti-CCR2 antibody [EPR19698] (Alexa Fluor® 647)
    See all CCR2 primary antibodies
  • Description

    Rabbit monoclonal [EPR19698] to CCR2 (Alexa Fluor® 647)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    This product was produced with the following immunogens:
    Recombinant fragment within Mouse CCR2 aa 1-100. The exact sequence is proprietary.
    Database link: P51683

    Recombinant fragment within Mouse CCR2 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: P51683

  • Positive control

    • ICC/IF: Raw264.7 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab225432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in Raw264.7 cells fixed with 4% formaldehyde (10 min)

Target

  • Function

    Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C C chemokine receptor type 2 antibody
    • C C CKR 2 antibody
    • C-C chemokine receptor type 2 antibody
    • C-C CKR-2 antibody
    • CC chemokine receptor type 2 antibody
    • CC CKR 2 antibody
    • CC-CKR-2 antibody
    • CCCKR2 antibody
    • CCR 2 antibody
    • CCR-2 antibody
    • CCR1L antibody
    • CCR2 antibody
    • CCR2_HUMAN antibody
    • CCR2A antibody
    • CCR2B antibody
    • CCR5L antibody
    • CD192 antibody
    • CD192 antigen antibody
    • Chemokine C C motif receptor 2 antibody
    • Chemokine CC Motif Receptor 2 antibody
    • CKR 2 antibody
    • CKR2 antibody
    • CKR2A antibody
    • CKR2B antibody
    • CMKBR2 antibody
    • MCP 1 R antibody
    • MCP-1-R antibody
    • MCP1 RECEPTOR antibody
    • MCP1R antibody
    • Monocyte chemoattractant protein 1 receptor antibody
    • Monocyte Chemotactic Protein 1 Receptor antibody
    • Monocyte Chemotactic Protein 1 Receptor antibody
    see all

Images

  • ab225432 staining CCR2 in Raw264.7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225432 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab225432 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab225432.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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