For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    ccr2-antibody-epr20844-15-ab273050.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Innate Immunity Macrophage / Inflamm.
Share by email
RecombinantRabMAb

Recombinant Anti-CCR2 antibody [EPR20844-15] (ab273050)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (2) Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)
  • Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844-15] (ab273050)
  • Flow Cytometry - Anti-CCR2 antibody [EPR20844-15] (ab273050)
  • Immunoprecipitation - Anti-CCR2 antibody [EPR20844-15] (ab273050)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)
  • Anti-CCR2 antibody [EPR20844-15] (ab273050)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20844-15] to CCR2
  • Suitable for: ICC/IF, IHC-P, IP, Flow Cyt
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

You may also be interested in

Primary
Product image
Anti-CD11b antibody [EPR1344] (ab133357)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Knockout
Product image
Human CCR2 knockout THP-1 cell line (ab273716)

View more associated products

Overview

  • Product name

    Anti-CCR2 antibody [EPR20844-15]
    See all CCR2 primary antibodies
  • Description

    Rabbit monoclonal [EPR20844-15] to CCR2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, IP, Flow Cytmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse spleen and lung tissue; Rat spleen tissue. ICC/IF: HEK-293T cells transfected with GFP tagged mouse CCR2 overexpression construct. Flow Cyt: HEK-293T cells transfected with GFP tagged mouse CCR2 overexpression construct. IP: Mouse lymph node tissue lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20844-15
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokine Rec. (CCR)
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cancer
    • Signal transduction
    • G protein signaling
    • GPCR
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Chemokines

Associated products

  • Alternative Versions

    • Anti-CCR2 antibody [EPR20844-15] - BSA and Azide free (ab273061)
    • Alexa Fluor® 594 Anti-CCR2 antibody [EPR20844] (ab286808)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab273050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/250.
IHC-P (2)
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.
Flow Cyt
1/600.
Notes
ICC/IF
1/250.
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.
Flow Cyt
1/600.
Application notes
Is unsuitable for WB.

Target

  • Function

    Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P41597 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 12772 Mouse
    • Entrez Gene: 60463 Rat
    • SwissProt: P51683 Mouse
    • SwissProt: O55193 Rat
    • Unigene: 6272 Mouse
    • Unigene: 211983 Rat
    • Alternative names

      • C C chemokine receptor type 2 antibody
      • C C CKR 2 antibody
      • C-C chemokine receptor type 2 antibody
      • C-C CKR-2 antibody
      • CC chemokine receptor type 2 antibody
      • CC CKR 2 antibody
      • CC-CKR-2 antibody
      • CCCKR2 antibody
      • CCR 2 antibody
      • CCR-2 antibody
      • CCR1L antibody
      • CCR2 antibody
      • CCR2_HUMAN antibody
      • CCR2A antibody
      • CCR2B antibody
      • CCR5L antibody
      • CD192 antibody
      • CD192 antigen antibody
      • Chemokine C C motif receptor 2 antibody
      • Chemokine CC Motif Receptor 2 antibody
      • CKR 2 antibody
      • CKR2 antibody
      • CKR2A antibody
      • CKR2B antibody
      • CMKBR2 antibody
      • MCP 1 R antibody
      • MCP-1-R antibody
      • MCP1 RECEPTOR antibody
      • MCP1R antibody
      • Monocyte chemoattractant protein 1 receptor antibody
      • Monocyte Chemotactic Protein 1 Receptor antibody
      • Monocyte Chemotactic Protein 1 Receptor antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CCR2 with ab273050 at 1/250 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse spleen. The section was incubated with ab273050 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844-15] (ab273050)
      Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844-15] (ab273050)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) (transfected with GFP tagged mouse CCR2 overexpression construct) cells labelling CCR2 with ab273050 at 1/250 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (Red). Confocal image showing membranous and cytoplasmic staining in HEK-293T cells transfected with with GFP tagged mouse CCR2 overexpression construct. GFP is shown in Green. The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.

    • Flow Cytometry - Anti-CCR2 antibody [EPR20844-15] (ab273050)
      Flow Cytometry - Anti-CCR2 antibody [EPR20844-15] (ab273050)

      Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) (transfected with GFP tagged mouse CCR2 overexpression construct) cells labelling CCR2 with ab273050 at 1/600 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

      Gated on viable cells.

    • Immunoprecipitation - Anti-CCR2 antibody [EPR20844-15] (ab273050)
      Immunoprecipitation - Anti-CCR2 antibody [EPR20844-15] (ab273050)

      CCR2 was immunoprecipitated from 0.35 mg Mouse lymph node tissue lysate with ab273050 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273050 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

      Lane 1: Mouse lymph node tissue lysate 10 ug.

      Lane 2: ab273050 IP in Mouse lymph node tissue lysate.

      Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab273050 in mouse lymph node tissue lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)

      Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CCR2 with ab273050 at 1/250 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat spleen. The section was incubated with ab273050 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)

      Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling CCR2 with ab273050 at 1/250 dilutionfollowed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse lung. The section was incubated with ab273050 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-CCR2 antibody [EPR20844-15] (ab273050)
      Anti-CCR2 antibody [EPR20844-15] (ab273050)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (1)

    Publishing research using ab273050? Please let us know so that we can cite the reference in this datasheet.

    ab273050 has been referenced in 1 publication.

    • Ngamsri KC  et al. Fractalkine Is Linked to the Necrosome Pathway in Acute Pulmonary Inflammation. Front Med (Lausanne) 8:591790 (2021). PubMed: 33791319

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CCR2 antibody [EPR20844-15]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    spleen
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
    Fixative
    10% NBF
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 21 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CCR2 antibody [EPR20844-15]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab208572
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 07 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.