Recombinant Anti-CCR2 antibody [EPR20844-15] (ab273050)
Overview
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Product name
Anti-CCR2 antibody [EPR20844-15]
See all CCR2 primary antibodies -
Description
Rabbit monoclonal [EPR20844-15] to CCR2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, IP, Flow Cytmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide within Mouse CCR2 aa 1-100. The exact sequence is proprietary.
Database link: P51683 -
Positive control
- IHC-P: Mouse spleen and lung tissue; Rat spleen tissue. ICC/IF: HEK-293T cells transfected with GFP tagged mouse CCR2 overexpression construct. FC: HEK-293T cells transfected with GFP tagged mouse CCR2 overexpression construct. IP: Mouse lymph node tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20844-15 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab273050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/250. | |
| IHC-P | 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| IP | 1/30. | |
| Flow Cyt | 1/600. |
Target
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Function
Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12772 Mouse
- Entrez Gene: 60463 Rat
- SwissProt: P51683 Mouse
- SwissProt: O55193 Rat
- Unigene: 6272 Mouse
- Unigene: 211983 Rat
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Alternative names
- C C chemokine receptor type 2 antibody
- C C CKR 2 antibody
- C-C chemokine receptor type 2 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)](https://www.abcam.com/ps/products/273/ab273050/Images/ab273050-1-anti-ccr2-antibody-epr2084415-immunohistochemistry-mouse-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CCR2 with ab273050 at 1/250 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse spleen. The section was incubated with ab273050 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844-15] (ab273050)](https://www.abcam.com/ps/products/273/ab273050/Images/ab273050-4-anti-ccr2-antibody-epr2084415-immunocytochemistry-293t.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844-15] (ab273050)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) (transfected with GFP tagged mouse CCR2 overexpression construct) cells labelling CCR2 with ab273050 at 1/250 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (Red). Confocal image showing membranous and cytoplasmic staining in HEK-293T cells transfected with with GFP tagged mouse CCR2 overexpression construct. GFP is shown in Green. The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.
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![Flow Cytometry - Anti-CCR2 antibody [EPR20844-15] (ab273050)](https://www.abcam.com/ps/products/273/ab273050/Images/ab273050-5-anti-ccr2-antibody-epr2084415-flow-cytometry-293t.jpg)
Flow Cytometry - Anti-CCR2 antibody [EPR20844-15] (ab273050)Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) (transfected with GFP tagged mouse CCR2 overexpression construct) cells labelling CCR2 with ab273050 at 1/600 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
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![Immunoprecipitation - Anti-CCR2 antibody [EPR20844-15] (ab273050)](https://www.abcam.com/ps/products/273/ab273050/Images/ab273050-6-anti-ccr2-antibody-epr2084415-immunoprecipitation-mouse-lymph-node-tissue-lysate10-ug.jpg)
Immunoprecipitation - Anti-CCR2 antibody [EPR20844-15] (ab273050)CCR2 was immunoprecipitated from 0.35 mg Mouse lymph node tissue lysate with ab273050 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273050 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.
Lane 1: Mouse lymph node tissue lysate 10 ug.
Lane 2: ab273050 IP in Mouse lymph node tissue lysate.
Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab273050 in mouse lymph node tissue lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)](https://www.abcam.com/ps/products/273/ab273050/Images/ab273050-3-anti-ccr2-antibody-epr2084415-immunohistochemistry-rat-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CCR2 with ab273050 at 1/250 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat spleen. The section was incubated with ab273050 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)](https://www.abcam.com/ps/products/273/ab273050/Images/ab273050-2-anti-ccr2-antibody-epr2084415-immunohistochemistry-mouse-lung.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling CCR2 with ab273050 at 1/250 dilutionfollowed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse lung. The section was incubated with ab273050 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References
ab273050 has not yet been referenced specifically in any publications.
