Recombinant Anti-CCR2 antibody [EPR20844-15] (ab273050)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)](https://www.abcam.com/ps/products/273/ab273050/Images/ab273050-1-anti-ccr2-antibody-epr2084415-immunohistochemistry-mouse-spleen.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20844-15] to CCR2
- Suitable for: ICC/IF, IHC-P, IP, Flow Cyt
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
-
Product name
Anti-CCR2 antibody [EPR20844-15]
See all CCR2 primary antibodies -
Description
Rabbit monoclonal [EPR20844-15] to CCR2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, IP, Flow Cytmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC-P: Mouse spleen and lung tissue; Rat spleen tissue. ICC/IF: HEK-293T cells transfected with GFP tagged mouse CCR2 overexpression construct. Flow Cyt: HEK-293T cells transfected with GFP tagged mouse CCR2 overexpression construct. IP: Mouse lymph node tissue lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20844-15 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab273050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/250.
|
|
| IHC-P | (2) |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| IP |
1/30.
|
|
| Flow Cyt |
1/600.
|
| Notes |
|---|
|
ICC/IF
1/250. |
|
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/30. |
|
Flow Cyt
1/600. |
Target
-
Function
Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 12772 Mouse
- Entrez Gene: 60463 Rat
- SwissProt: P51683 Mouse
- SwissProt: O55193 Rat
- Unigene: 6272 Mouse
- Unigene: 211983 Rat
-
Alternative names
- C C chemokine receptor type 2 antibody
- C C CKR 2 antibody
- C-C chemokine receptor type 2 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CCR2 with ab273050 at 1/250 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse spleen. The section was incubated with ab273050 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844-15] (ab273050)](https://www.abcam.com/ps/products/273/ab273050/Images/ab273050-4-anti-ccr2-antibody-epr2084415-immunocytochemistry-293t.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844-15] (ab273050)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) (transfected with GFP tagged mouse CCR2 overexpression construct) cells labelling CCR2 with ab273050 at 1/250 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (Red). Confocal image showing membranous and cytoplasmic staining in HEK-293T cells transfected with with GFP tagged mouse CCR2 overexpression construct. GFP is shown in Green. The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.
-
![Flow Cytometry - Anti-CCR2 antibody [EPR20844-15] (ab273050)](https://www.abcam.com/ps/products/273/ab273050/Images/ab273050-5-anti-ccr2-antibody-epr2084415-flow-cytometry-293t.jpg)
Flow Cytometry - Anti-CCR2 antibody [EPR20844-15] (ab273050)Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) (transfected with GFP tagged mouse CCR2 overexpression construct) cells labelling CCR2 with ab273050 at 1/600 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
-
![Immunoprecipitation - Anti-CCR2 antibody [EPR20844-15] (ab273050)](https://www.abcam.com/ps/products/273/ab273050/Images/ab273050-6-anti-ccr2-antibody-epr2084415-immunoprecipitation-mouse-lymph-node-tissue-lysate10-ug.jpg)
Immunoprecipitation - Anti-CCR2 antibody [EPR20844-15] (ab273050)CCR2 was immunoprecipitated from 0.35 mg Mouse lymph node tissue lysate with ab273050 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273050 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.
Lane 1: Mouse lymph node tissue lysate 10 ug.
Lane 2: ab273050 IP in Mouse lymph node tissue lysate.
Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab273050 in mouse lymph node tissue lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)](https://www.abcam.com/ps/products/273/ab273050/Images/ab273050-3-anti-ccr2-antibody-epr2084415-immunohistochemistry-rat-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CCR2 with ab273050 at 1/250 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat spleen. The section was incubated with ab273050 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)](https://www.abcam.com/ps/products/273/ab273050/Images/ab273050-2-anti-ccr2-antibody-epr2084415-immunohistochemistry-mouse-lung.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] (ab273050)Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling CCR2 with ab273050 at 1/250 dilutionfollowed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse lung. The section was incubated with ab273050 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Anti-CCR2 antibody [EPR20844-15] (ab273050)](https://www.abcam.com/ps/products/273/ab273050/Images/ab273050-7-benefits-of-recombinant-antibodies.png)
Anti-CCR2 antibody [EPR20844-15] (ab273050)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (1)
ab273050 has been referenced in 1 publication.
- Ngamsri KC et al. Fractalkine Is Linked to the Necrosome Pathway in Acute Pulmonary Inflammation. Front Med (Lausanne) 8:591790 (2021). PubMed: 33791319
