Overview

  • Product name

    Anti-CCR2 antibody [EPR20844]
    See all CCR2 primary antibodies
  • Description

    Rabbit monoclonal [EPR20844] to CCR2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse CCR2 aa 1-100. The exact sequence is proprietary.
    Database link: P51683

  • Positive control

    • ICC/IF: HEK-293T lysate. Flow: Mouse primary splenocytes; HEK-293T transfected with GFP-tagged CCR2 expression vector.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab216863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
Flow Cyt 1/600.

Target

  • Function

    Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C C chemokine receptor type 2 antibody
    • C C CKR 2 antibody
    • C-C chemokine receptor type 2 antibody
    • C-C CKR-2 antibody
    • CC chemokine receptor type 2 antibody
    • CC CKR 2 antibody
    • CC-CKR-2 antibody
    • CCCKR2 antibody
    • CCR 2 antibody
    • CCR-2 antibody
    • CCR1L antibody
    • CCR2 antibody
    • CCR2_HUMAN antibody
    • CCR2A antibody
    • CCR2B antibody
    • CCR5L antibody
    • CD192 antibody
    • CD192 antigen antibody
    • Chemokine C C motif receptor 2 antibody
    • Chemokine CC Motif Receptor 2 antibody
    • CKR 2 antibody
    • CKR2 antibody
    • CKR2A antibody
    • CKR2B antibody
    • CMKBR2 antibody
    • MCP 1 R antibody
    • MCP-1-R antibody
    • MCP1 RECEPTOR antibody
    • MCP1R antibody
    • Monocyte chemoattractant protein 1 receptor antibody
    • Monocyte Chemotactic Protein 1 Receptor antibody
    • Monocyte Chemotactic Protein 1 Receptor antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling CCR2 with ab216863 at 1/100 dilution, followed by AlexaFluor®647 Goat anti-Rabbit secondary (ab150079) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in 293T cells transfected with GFP-tagged CCR2 expression vector. DAPI was used as the nuclear countertsain (Blue).
    Secondary antibody only control:
    Control 1: AlexaFluor®647 Goat anti-Rabbit secondary (ab150079) secondary antibody in GFP transfected HEK-293T cells.
    Control 2: AlexaFluor®647 Goat anti-Rabbit secondary (ab150079) secondary antibody in CCR2-GFP transfected cells.

  • Flow cytometric analysis of Mouse primary splenocytes labelling CCR2 with ab216863 at 1/600 dilution (Right) compared with Rabbit monoclonal IgG (ab172730) (Left) as a an isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody. Gated on viable cells.

  • Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) transfected with GFP-tagged CCR2 expression vector, cell line labelling CCR2 with ab216863 at 1/600 dilution (Right) and an isotype control Rabbit monoclonal IgG (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody. Gated on viable cells. Only GFP(+) population give a positive signal.

References

ab216863 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab216863.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up