Recombinant Anti-CCR2 antibody [EPR20844] (ab216863)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20844] to CCR2
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse
Overview
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Product name
Anti-CCR2 antibody [EPR20844]
See all CCR2 primary antibodies -
Description
Rabbit monoclonal [EPR20844] to CCR2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide within Mouse CCR2 aa 1-100. The exact sequence is proprietary.
Database link: P51683 -
Positive control
- ICC/IF: 293T cell line. Flow: Mouse primary splenocytes; 293T transfected with GFP-tagged CCR2 expression vector.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20844 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab216863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. | |
| Flow Cyt | 1/600. |
Target
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Function
Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12772 Mouse
- SwissProt: P51683 Mouse
- Unigene: 6272 Mouse
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Alternative names
- C C chemokine receptor type 2 antibody
- C C CKR 2 antibody
- C-C chemokine receptor type 2 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844] (ab216863)](https://www.abcam.com/ps/products/216/ab216863/Images/ab216863-306853-anti-ccr2-antibody-epr20844-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844] (ab216863)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling CCR2 with ab216863 at 1/100 dilution, followed by AlexaFluor®647 Goat anti-Rabbit secondary (ab150079) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in 293T cells transfected with GFP-tagged CCR2 expression vector. DAPI was used as the nuclear countertsain (Blue).
Secondary antibody only control:
Control 1: AlexaFluor®647 Goat anti-Rabbit secondary (ab150079) secondary antibody in GFP transfected HEK-293T cells.
Control 2: AlexaFluor®647 Goat anti-Rabbit secondary (ab150079) secondary antibody in CCR2-GFP transfected cells. -
![Flow Cytometry - Anti-CCR2 antibody [EPR20844] (ab216863)](https://www.abcam.com/ps/products/216/ab216863/Images/ab216863-306851-anti-ccr2-antibody-epr20844-flow-cytometry.jpg)
Flow Cytometry - Anti-CCR2 antibody [EPR20844] (ab216863)Flow cytometric analysis of Mouse primary splenocytes labelling CCR2 with ab216863 at 1/600 dilution (Right) compared with Rabbit monoclonal IgG (ab172730) (Left) as a an isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody. Gated on viable cells.
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![Flow Cytometry - Anti-CCR2 antibody [EPR20844] (ab216863)](https://www.abcam.com/ps/products/216/ab216863/Images/ab216863-306852-anti-ccr2-antibody-epr20844-flow-cytometry.jpg)
Flow Cytometry - Anti-CCR2 antibody [EPR20844] (ab216863)Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) transfected with GFP-tagged CCR2 expression vector, cell line labelling CCR2 with ab216863 at 1/600 dilution (Right) and an isotype control Rabbit monoclonal IgG (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody. Gated on viable cells. Only GFP(+) population give a positive signal.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab216863 has not yet been referenced specifically in any publications.
