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    ccr2-antibody-epr20844-ab216863.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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RecombinantRabMAb

Recombinant Anti-CCR2 antibody [EPR20844] (ab216863)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844] (ab216863)
  • Flow Cytometry - Anti-CCR2 antibody [EPR20844] (ab216863)
  • Flow Cytometry - Anti-CCR2 antibody [EPR20844] (ab216863)
  • Anti-CCR2 antibody [EPR20844] (ab216863)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20844] to CCR2
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Mouse, Recombinant fragment

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free PE

You may also be interested in

Protein
Product image
Recombinant Human CCR2 protein (ab114168)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Knockout
Product image
Human CCR2 knockout THP-1 cell line (ab273716)

View more associated products

Overview

  • Product name

    Anti-CCR2 antibody [EPR20844]
    See all CCR2 primary antibodies
  • Description

    Rabbit monoclonal [EPR20844] to CCR2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Recombinant fragment
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: 293T cell line. Flow: Mouse primary splenocytes; 293T transfected with GFP-tagged CCR2 expression vector.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20844
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokine Rec. (CCR)
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cancer
    • Signal transduction
    • G protein signaling
    • GPCR
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Chemokines

Associated products

  • Alternative Versions

    • Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)
    • Alexa Fluor® 488 Anti-CCR2 antibody [EPR20844] (ab245896)
    • Alexa Fluor® 647 Anti-CCR2 antibody [EPR20844] (ab245897)
    • APC Anti-CCR2 antibody [EPR20844] (ab245898)
    • PE Anti-CCR2 antibody [EPR20844] (ab245899)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CCR2 protein (ab114168)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab216863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.
Flow Cyt
1/600.
Notes
ICC/IF
1/100.
Flow Cyt
1/600.

Target

  • Function

    Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P41597 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 12772 Mouse
    • SwissProt: P51683 Mouse
    • Unigene: 6272 Mouse
    • Alternative names

      • C C chemokine receptor type 2 antibody
      • C C CKR 2 antibody
      • C-C chemokine receptor type 2 antibody
      • C-C CKR-2 antibody
      • CC chemokine receptor type 2 antibody
      • CC CKR 2 antibody
      • CC-CKR-2 antibody
      • CCCKR2 antibody
      • CCR 2 antibody
      • CCR-2 antibody
      • CCR1L antibody
      • CCR2 antibody
      • CCR2_HUMAN antibody
      • CCR2A antibody
      • CCR2B antibody
      • CCR5L antibody
      • CD192 antibody
      • CD192 antigen antibody
      • Chemokine C C motif receptor 2 antibody
      • Chemokine CC Motif Receptor 2 antibody
      • CKR 2 antibody
      • CKR2 antibody
      • CKR2A antibody
      • CKR2B antibody
      • CMKBR2 antibody
      • MCP 1 R antibody
      • MCP-1-R antibody
      • MCP1 RECEPTOR antibody
      • MCP1R antibody
      • Monocyte chemoattractant protein 1 receptor antibody
      • Monocyte Chemotactic Protein 1 Receptor antibody
      • Monocyte Chemotactic Protein 1 Receptor antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844] (ab216863)
      Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844] (ab216863)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling CCR2 with ab216863 at 1/100 dilution, followed by Alexa Fluor® 647 Goat anti-Rabbit secondary (ab150079) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in 293T cells transfected with GFP-tagged CCR2 expression vector. DAPI was used as the nuclear countertsain (Blue).

      Secondary antibody only control:
      Control 1: Alexa Fluor® 647 Goat anti-Rabbit secondary (ab150079) secondary antibody in GFP transfected HEK-293T cells.
      Control 2: Alexa Fluor® 647 Goat anti-Rabbit secondary (ab150079) secondary antibody in CCR2-GFP transfected cells.

    • Flow Cytometry - Anti-CCR2 antibody [EPR20844] (ab216863)
      Flow Cytometry - Anti-CCR2 antibody [EPR20844] (ab216863)

      Flow cytometric analysis of Mouse primary splenocytes labelling CCR2 with ab216863 at 1/600 dilution (Right) compared with Rabbit monoclonal IgG (ab172730) (Left) as a an isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody. Gated on viable cells.

    • Flow Cytometry - Anti-CCR2 antibody [EPR20844] (ab216863)
      Flow Cytometry - Anti-CCR2 antibody [EPR20844] (ab216863)

      Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) transfected with GFP-tagged CCR2 expression vector, cell line labelling CCR2 with ab216863 at 1/600 dilution (Right) and an isotype control Rabbit monoclonal IgG (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody. Gated on viable cells. Only GFP(+) population give a positive signal.

    • Anti-CCR2 antibody [EPR20844] (ab216863)
      Anti-CCR2 antibody [EPR20844] (ab216863)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab216863? Please let us know so that we can cite the reference in this datasheet.

    ab216863 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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