Recombinant Anti-CCR2 antibody [EPR20844] (ab216863)
![Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844] (ab216863)](https://www.abcam.com/ps/products/216/ab216863/Images/ab216863-306853-anti-ccr2-antibody-epr20844-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844] (ab216863)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20844] to CCR2
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse, Recombinant fragment
Related conjugates and formulations
Overview
-
Product name
Anti-CCR2 antibody [EPR20844]
See all CCR2 primary antibodies -
Description
Rabbit monoclonal [EPR20844] to CCR2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- ICC/IF: 293T cell line. Flow: Mouse primary splenocytes; 293T transfected with GFP-tagged CCR2 expression vector.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20844 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100.
|
|
| Flow Cyt |
1/600.
|
| Notes |
|---|
|
ICC/IF
1/100. |
|
Flow Cyt
1/600. |
Target
-
Function
Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 12772 Mouse
- SwissProt: P51683 Mouse
- Unigene: 6272 Mouse
-
Alternative names
- C C chemokine receptor type 2 antibody
- C C CKR 2 antibody
- C-C chemokine receptor type 2 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844] (ab216863)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling CCR2 with ab216863 at 1/100 dilution, followed by Alexa Fluor® 647 Goat anti-Rabbit secondary (ab150079) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in 293T cells transfected with GFP-tagged CCR2 expression vector. DAPI was used as the nuclear countertsain (Blue).
Secondary antibody only control:
Control 1: Alexa Fluor® 647 Goat anti-Rabbit secondary (ab150079) secondary antibody in GFP transfected HEK-293T cells.
Control 2: Alexa Fluor® 647 Goat anti-Rabbit secondary (ab150079) secondary antibody in CCR2-GFP transfected cells. -
![Flow Cytometry - Anti-CCR2 antibody [EPR20844] (ab216863)](https://www.abcam.com/ps/products/216/ab216863/Images/ab216863-306851-anti-ccr2-antibody-epr20844-flow-cytometry.jpg)
Flow Cytometry - Anti-CCR2 antibody [EPR20844] (ab216863)Flow cytometric analysis of Mouse primary splenocytes labelling CCR2 with ab216863 at 1/600 dilution (Right) compared with Rabbit monoclonal IgG (ab172730) (Left) as a an isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody. Gated on viable cells.
-
![Flow Cytometry - Anti-CCR2 antibody [EPR20844] (ab216863)](https://www.abcam.com/ps/products/216/ab216863/Images/ab216863-306852-anti-ccr2-antibody-epr20844-flow-cytometry.jpg)
Flow Cytometry - Anti-CCR2 antibody [EPR20844] (ab216863)Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) transfected with GFP-tagged CCR2 expression vector, cell line labelling CCR2 with ab216863 at 1/600 dilution (Right) and an isotype control Rabbit monoclonal IgG (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody. Gated on viable cells. Only GFP(+) population give a positive signal.
-
![Anti-CCR2 antibody [EPR20844] (ab216863)](https://www.abcam.com/ps/products/216/ab216863/Images/ab216863-4-benefits-of-recombinant-antibodies.png)
Anti-CCR2 antibody [EPR20844] (ab216863)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab216863 has not yet been referenced specifically in any publications.