Recombinant
RabMAb

Recombinant Anti-CCR2 antibody [EPR20844] (ab216863)

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Overview

  • Product name

    Anti-CCR2 antibody [EPR20844]
    See all CCR2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20844] to CCR2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Synthetic peptide within Mouse CCR2 aa 1-100. The exact sequence is proprietary.
    Database link: P51683

  • Positive control

    • ICC/IF: HEK-293T lysate. Flow: Mouse primary splenocytes; HEK-293T transfected with GFP-tagged CCR2 expression vector.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab216863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
Flow Cyt 1/600.

Target

  • Function

    Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.

  • Post-translational
    modifications

    N-glycosylated.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • C C chemokine receptor type 2 antibody
      • C C CKR 2 antibody
      • C-C chemokine receptor type 2 antibody
      • C-C CKR-2 antibody
      • CC chemokine receptor type 2 antibody
      • CC CKR 2 antibody
      • CC-CKR-2 antibody
      • CCCKR2 antibody
      • CCR 2 antibody
      • CCR-2 antibody
      • CCR1L antibody
      • CCR2 antibody
      • CCR2_HUMAN antibody
      • CCR2A antibody
      • CCR2B antibody
      • CCR5L antibody
      • CD192 antibody
      • CD192 antigen antibody
      • Chemokine C C motif receptor 2 antibody
      • Chemokine CC Motif Receptor 2 antibody
      • CKR 2 antibody
      • CKR2 antibody
      • CKR2A antibody
      • CKR2B antibody
      • CMKBR2 antibody
      • MCP 1 R antibody
      • MCP-1-R antibody
      • MCP1 RECEPTOR antibody
      • MCP1R antibody
      • Monocyte chemoattractant protein 1 receptor antibody
      • Monocyte Chemotactic Protein 1 Receptor antibody
      • Monocyte Chemotactic Protein 1 Receptor antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844] (ab216863)
      Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844] (ab216863)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling CCR2 with ab216863 at 1/100 dilution, followed by AlexaFluor®647 Goat anti-Rabbit secondary (ab150079) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in 293T cells transfected with GFP-tagged CCR2 expression vector. DAPI was used as the nuclear countertsain (Blue).
      Secondary antibody only control:
      Control 1: AlexaFluor®647 Goat anti-Rabbit secondary (ab150079) secondary antibody in GFP transfected HEK-293T cells.
      Control 2: AlexaFluor®647 Goat anti-Rabbit secondary (ab150079) secondary antibody in CCR2-GFP transfected cells.

    • Flow Cytometry - Anti-CCR2 antibody [EPR20844] (ab216863)
      Flow Cytometry - Anti-CCR2 antibody [EPR20844] (ab216863)

      Flow cytometric analysis of Mouse primary splenocytes labelling CCR2 with ab216863 at 1/600 dilution (Right) compared with Rabbit monoclonal IgG (ab172730) (Left) as a an isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody. Gated on viable cells.

    • Flow Cytometry - Anti-CCR2 antibody [EPR20844] (ab216863)
      Flow Cytometry - Anti-CCR2 antibody [EPR20844] (ab216863)

      Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) transfected with GFP-tagged CCR2 expression vector, cell line labelling CCR2 with ab216863 at 1/600 dilution (Right) and an isotype control Rabbit monoclonal IgG (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody. Gated on viable cells. Only GFP(+) population give a positive signal.

    References

    ab216863 has not yet been referenced specifically in any publications.

    Publishing research using ab216863? Please let us know so that we can cite the reference in this datasheet.

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