Recombinant Alexa Fluor® 488 Anti-CCR2 antibody [EPR20844] (ab245896)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR20844] to CCR2
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
Overview
-
Product name
Alexa Fluor® 488 Anti-CCR2 antibody [EPR20844]
See all CCR2 primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EPR20844] to CCR2 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- ICC/IF: Mouse CCR2 with RFP tag transfected HEK-293T cells. Flow Cyt: Splenocytes (Mouse) cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20844 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
- Anti-CCR2 antibody [EPR20844] (ab216863)
- Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)
- Alexa Fluor® 647 Anti-CCR2 antibody [EPR20844] (ab245897)
- APC Anti-CCR2 antibody [EPR20844] (ab245898)
- PE Anti-CCR2 antibody [EPR20844] (ab245899)
- Alexa Fluor® 594 Anti-CCR2 antibody [EPR20844] (ab286808)
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/200.
This product gave a positive signal in RFP transfected 293T fixed with 4% formaldehyde (10 min). |
|
Flow Cyt |
1/2500.
|
Notes |
---|
ICC/IF
1/200. This product gave a positive signal in RFP transfected 293T fixed with 4% formaldehyde (10 min). |
Flow Cyt
1/2500. |
Target
-
Function
Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 12772 Mouse
- SwissProt: P51683 Mouse
- Unigene: 6272 Mouse
-
Alternative names
- C C chemokine receptor type 2 antibody
- C C CKR 2 antibody
- C-C chemokine receptor type 2 antibody
see all
Images
-
C57BL/6 mouse splenocytes stained with ab245896 (right) or Rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) (left). C57BL/6 Mouse splenocytes were incubated for 30 min on ice in 1x PBS containing 10 µg/ml anti CD16/32 antibody and 10% normal goat serum to block FC receptors and non-specific protein-protein interactions followed by the antibody (ab245896) or Rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) (1x 106 in 100 µl at 0.2 µg/ml (1/2500)) for 30 min on ice. The cells were simultaneously stained with Ly6G/GR1 PerCP conjugated antibody (ab236728).
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488 nm) and 530/30 bandpass filter.
Events were gated on viable lymphocytes.
-
ab245896 staining CCR2 in HEK-293T cells transfected with mouse CCR2 with RFP tag. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab245896 at 1/200 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue) and RFP is shown in red.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab245896 has not yet been referenced specifically in any publications.