Recombinant Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)
![Flow Cytometry - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)](https://www.abcam.com/ps/products/233/ab233701/Images/ab233701-321376-anti-ccr2-antibody-epr20844-flow-cytometry.jpg "Flow Cytometry - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20844] to CCR2 - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse
Related conjugates and formulations
Overview
-
Product name
Anti-CCR2 antibody [EPR20844] - BSA and Azide free
See all CCR2 primary antibodies -
Description
Rabbit monoclonal [EPR20844] to CCR2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- ICC/IF: HEK-293T lysate transfected with GFP-tagged CCR2. Flow Cyt: HEK-293T cells transfected with GFP-tagged CCR2 and mouse primary splenocytes.
-
General notes
ab233701 is the carrier-free version of ab216863.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20844 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
- Anti-CCR2 antibody [EPR20844] (ab216863)
- Alexa Fluor® 488 Anti-CCR2 antibody [EPR20844] (ab245896)
- Alexa Fluor® 647 Anti-CCR2 antibody [EPR20844] (ab245897)
- APC Anti-CCR2 antibody [EPR20844] (ab245898)
- PE Anti-CCR2 antibody [EPR20844] (ab245899)
- Alexa Fluor® 594 Anti-CCR2 antibody [EPR20844] (ab286808)
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233701 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. |
Target
-
Function
Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 12772 Mouse
- SwissProt: P51683 Mouse
- Unigene: 6272 Mouse
-
Alternative names
- C C chemokine receptor type 2 antibody
- C C CKR 2 antibody
- C-C chemokine receptor type 2 antibody
see all
Images
-
Flow Cytometry - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)
Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) transfected with GFP-tagged CCR2 expression vector, cell line labelling CCR2 with ab216863 at 1/600 dilution (Right) and an isotype control Rabbit monoclonal IgG (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody. Gated on viable cells. Only GFP(+) population give a positive signal.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216863).
-
![Flow Cytometry - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)](https://www.abcam.com/ps/products/233/ab233701/Images/ab233701-321375-anti-ccr2-antibody-epr20844-flow-cytometry.jpg)
Flow Cytometry - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)Flow cytometric analysis of Mouse primary splenocytes labelling CCR2 with ab216863 at 1/600 dilution (Right) compared with Rabbit monoclonal IgG (ab172730) (Left) as a an isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody. Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216863).
-
![Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)](https://www.abcam.com/ps/products/233/ab233701/Images/ab233701-306949-anti-ccr2-antibody-epr20844-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling CCR2 with ab216863 at 1/100 dilution, followed by Alexa Fluor® 647 Goat anti-Rabbit secondary (ab150079) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in 293T cells transfected with GFP-tagged CCR2 expression vector. DAPI was used as the nuclear countertsain (Blue).
Secondary antibody only control:
Control 1: Alexa Fluor® 647 Goat anti-Rabbit secondary (ab150079) secondary antibody in GFP transfected HEK-293T cells.
Control 2: Alexa Fluor® 647 Goat anti-Rabbit secondary (ab150079) secondary antibody in CCR2-GFP transfected cells.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233701).
-
![Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)](https://www.abcam.com/ps/products/233/ab233701/Images/ab233701-4-benefits-of-recombinant-antibodies.png)
Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab233701 has not yet been referenced specifically in any publications.
