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    ccr2-antibody-epr20844-bsa-and-azide-free-ab233701.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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RecombinantRabMAb

Recombinant Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)

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  • Certificate of Compliance
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Flow Cytometry - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)
  • Flow Cytometry - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)
  • Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)
  • Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20844] to CCR2 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 APC PE Unconjugated

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Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
Primary
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Anti-CCR2 antibody [EPR20844] (ab216863)
Knockout
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Human CCR2 knockout THP-1 cell line (ab273716)

View more associated products

Overview

  • Product name

    Anti-CCR2 antibody [EPR20844] - BSA and Azide free
    See all CCR2 primary antibodies
  • Description

    Rabbit monoclonal [EPR20844] to CCR2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HEK-293T lysate transfected with GFP-tagged CCR2. Flow Cyt: HEK-293T cells transfected with GFP-tagged CCR2 and mouse primary splenocytes.
  • General notes

    ab233701 is the carrier-free version of ab216863.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20844
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokine Rec. (CCR)
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cancer
    • Signal transduction
    • G protein signaling
    • GPCR
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Chemokines

Associated products

  • Alternative Versions

    • Anti-CCR2 antibody [EPR20844] (ab216863)
    • Alexa Fluor® 488 Anti-CCR2 antibody [EPR20844] (ab245896)
    • Alexa Fluor® 647 Anti-CCR2 antibody [EPR20844] (ab245897)
    • APC Anti-CCR2 antibody [EPR20844] (ab245898)
    • PE Anti-CCR2 antibody [EPR20844] (ab245899)
    • Alexa Fluor® 594 Anti-CCR2 antibody [EPR20844] (ab286808)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CCR2 protein (ab114168)
  • Related Products

    • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab233701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
Notes
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Target

  • Function

    Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P41597 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 12772 Mouse
    • SwissProt: P51683 Mouse
    • Unigene: 6272 Mouse
    • Alternative names

      • C C chemokine receptor type 2 antibody
      • C C CKR 2 antibody
      • C-C chemokine receptor type 2 antibody
      • C-C CKR-2 antibody
      • CC chemokine receptor type 2 antibody
      • CC CKR 2 antibody
      • CC-CKR-2 antibody
      • CCCKR2 antibody
      • CCR 2 antibody
      • CCR-2 antibody
      • CCR1L antibody
      • CCR2 antibody
      • CCR2_HUMAN antibody
      • CCR2A antibody
      • CCR2B antibody
      • CCR5L antibody
      • CD192 antibody
      • CD192 antigen antibody
      • Chemokine C C motif receptor 2 antibody
      • Chemokine CC Motif Receptor 2 antibody
      • CKR 2 antibody
      • CKR2 antibody
      • CKR2A antibody
      • CKR2B antibody
      • CMKBR2 antibody
      • MCP 1 R antibody
      • MCP-1-R antibody
      • MCP1 RECEPTOR antibody
      • MCP1R antibody
      • Monocyte chemoattractant protein 1 receptor antibody
      • Monocyte Chemotactic Protein 1 Receptor antibody
      • Monocyte Chemotactic Protein 1 Receptor antibody
      see all

    Images

    • Flow Cytometry - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)
      Flow Cytometry - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)

      Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) transfected with GFP-tagged CCR2 expression vector, cell line labelling CCR2 with ab216863 at 1/600 dilution (Right) and an isotype control Rabbit monoclonal IgG (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody. Gated on viable cells. Only GFP(+) population give a positive signal.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216863).

    • Flow Cytometry - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)
      Flow Cytometry - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)

      Flow cytometric analysis of Mouse primary splenocytes labelling CCR2 with ab216863 at 1/600 dilution (Right) compared with Rabbit monoclonal IgG (ab172730) (Left) as a an isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody. Gated on viable cells.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216863).

    • Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)
      Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling CCR2 with ab216863 at 1/100 dilution, followed by Alexa Fluor® 647 Goat anti-Rabbit secondary (ab150079) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in 293T cells transfected with GFP-tagged CCR2 expression vector. DAPI was used as the nuclear countertsain (Blue).

      Secondary antibody only control:
      Control 1: Alexa Fluor® 647 Goat anti-Rabbit secondary (ab150079) secondary antibody in GFP transfected HEK-293T cells.
      Control 2: Alexa Fluor® 647 Goat anti-Rabbit secondary (ab150079) secondary antibody in CCR2-GFP transfected cells.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233701).

    • Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)
      Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab233701? Please let us know so that we can cite the reference in this datasheet.

    ab233701 has not yet been referenced specifically in any publications.

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