Recombinant Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)
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Overview
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Product name
Anti-CCR2 antibody [EPR20844] - BSA and Azide free
See all CCR2 primary antibodies -
Description
Rabbit monoclonal [EPR20844] to CCR2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Mouse CCR2 aa 1-100.
Database link: P51683 -
Positive control
- ICC/IF: HEK-293T lysate.
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General notes
Ab233701 is the carrier-free version of ab216863. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab233701 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20844 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab233701 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. |
Target
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Function
Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12772 Mouse
- SwissProt: P51683 Mouse
- Unigene: 6272 Mouse
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Alternative names
- C C chemokine receptor type 2 antibody
- C C CKR 2 antibody
- C-C chemokine receptor type 2 antibody
see all
Images
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![Flow Cytometry - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)](https://www.abcam.com/ps/products/233/ab233701/Images/ab233701-321376-anti-ccr2-antibody-epr20844-flow-cytometry.jpg)
Flow Cytometry - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) transfected with GFP-tagged CCR2 expression vector, cell line labelling CCR2 with ab216863 at 1/600 dilution (Right) and an isotype control Rabbit monoclonal IgG (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody. Gated on viable cells. Only GFP(+) population give a positive signal.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216863).
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![Flow Cytometry - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)](https://www.abcam.com/ps/products/233/ab233701/Images/ab233701-321375-anti-ccr2-antibody-epr20844-flow-cytometry.jpg)
Flow Cytometry - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)Flow cytometric analysis of Mouse primary splenocytes labelling CCR2 with ab216863 at 1/600 dilution (Right) compared with Rabbit monoclonal IgG (ab172730) (Left) as a an isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody. Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216863).
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![Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)](https://www.abcam.com/ps/products/233/ab233701/Images/ab233701-306949-anti-ccr2-antibody-epr20844-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling CCR2 with ab216863 at 1/100 dilution, followed by AlexaFluor®647 Goat anti-Rabbit secondary (ab150079) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in 293T cells transfected with GFP-tagged CCR2 expression vector. DAPI was used as the nuclear countertsain (Blue).
Secondary antibody only control:
Control 1: AlexaFluor®647 Goat anti-Rabbit secondary (ab150079) secondary antibody in GFP transfected HEK-293T cells.
Control 2: AlexaFluor®647 Goat anti-Rabbit secondary (ab150079) secondary antibody in CCR2-GFP transfected cells.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233701).
Datasheets and documents
References
ab233701 has not yet been referenced specifically in any publications.
