Product nameAnti-CCR3 antibody
See all CCR3 primary antibodies
DescriptionGoat polyclonal to CCR3
SpecificityPeptide sequence is < 50 % identical to other human chemokine receptors in this region.
Tested applicationsSuitable for: ICC, IHC-P, WBmore details
Species reactivityReacts with: Human
- This anitbody gave a positive signal in human lung, ovary and thymus tissue lysates.
Human CCR 3 is a receptor for CC chemokine eotaxin-1 and eotaxin-2. CCR 3 is constitutively expressed on eosinophils, small intestine and colon. The human CCR 3 protein contains 356 amino acids.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 0.1% BSA, 0.11% Dibasic monohydrogen potassium phosphate, 0.05% Monobasic dihydrogen potassium phosphate, 0.82% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Primary antibody notesHuman CCR 3 is a receptor for CC chemokine eotaxin-1 and eotaxin-2. CCR 3 is constitutively expressed on eosinophils, small intestine and colon. The human CCR 3 protein contains 356 amino acids.
Our Abpromise guarantee covers the use of ab1667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).|
FunctionReceptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
Tissue specificityIn eosinophils as well as trace amounts in neutrophils and monocytes.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- B chemokine receptor antibody
- b-chemokine receptor antibody
- C C chemokine receptor type 3 antibody
Immunocytochemistry on human peripheral blood cells (acetone fixed cells).
All lanes : Anti-CCR3 antibody (ab1667) at 1 µg/ml
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Human ovary tissue lysate - total protein (ab30222)
Lane 3 : Human thymus tissue lysate - total protein (ab30146)
Lysates/proteins at 10 µg per lane.
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
This product has been referenced in:
- Jäger K et al. Expression of Neural Crest Markers GLDC and ERRFI1 is Correlated with Melanoma Prognosis. Cancers (Basel) 11:N/A (2019). Read more (PubMed: 30641895) »
- Saito Y et al. Synbiotics suppress colitis-induced tumorigenesis in a colon-specific cancer mouse model. PLoS One 14:e0216393 (2019). Read more (PubMed: 31242213) »