Overview

  • Product name
  • Description
    Goat polyclonal to CCR3
  • Host species
    Goat
  • Specificity
    Peptide sequence is < 50 % identical to other human chemokine receptors in this region.
  • Tested applications
    Suitable for: ICC, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: C-VETFGTTSYYDDVGLLCEKADTRALMA, corresponding to N terminal amino acids 8-34 of Human CCR3.

  • Positive control
    • This anitbody gave a positive signal in human lung, ovary and thymus tissue lysates.
  • General notes


    Human CCR 3 is a receptor for CC chemokine eotaxin-1 and eotaxin-2. CCR 3 is constitutively expressed on eosinophils, small intestine and colon. The human CCR 3 protein contains 356 amino acids.

Properties

Applications

Our Abpromise guarantee covers the use of ab1667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/150.
IHC-P 1/150.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

Target

  • Function
    Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
  • Tissue specificity
    In eosinophils as well as trace amounts in neutrophils and monocytes.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B chemokine receptor antibody
    • b-chemokine receptor antibody
    • C C chemokine receptor type 3 antibody
    • C C CKR 3 antibody
    • C-C chemokine receptor type 3 antibody
    • C-C CKR-3 antibody
    • CC chemokine receptor 3 antibody
    • CC chemokine receptor type 3 antibody
    • CC CKR 3 antibody
    • CC R3 antibody
    • CC-CKR-3 antibody
    • CCCKR3 antibody
    • CCR 3 antibody
    • CCR-3 antibody
    • CCR3 antibody
    • CCR3_HUMAN antibody
    • CD 193 antibody
    • CD193 antibody
    • CD193 antigen antibody
    • Chemokine (C C motif) receptor 3 antibody
    • chemokine (C-C motif) receptor 3 antibody
    • Chemokine (CC) receptor 1 like 2 antibody
    • Chemokine CC motif receptor 3 antibody
    • Chemokine receptor 3 antibody
    • CK R3 antibody
    • CKR 3 antibody
    • CKR3 antibody
    • CMKB R3 antibody
    • CMKBR 3 antibody
    • CMKBR1L2 antibody
    • CMKBR3 antibody
    • Eosinophil CC chemokine receptor 3 antibody
    • Eosinophil eotaxin receptor antibody
    • Eotaxin receptor antibody
    • Macrophage inflammatory protein 1 alpha receptor like 2 antibody
    • MGC102841 antibody
    • MIP1 Alpha RL2 antibody
    see all

Images

  • Immunocytochemistry on human peripheral blood cells (acetone fixed cells).
  • All lanes : Anti-CCR3 antibody (ab1667) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human ovary tissue lysate - total protein (ab30222)
    Lane 3 : Human thymus tissue lysate - total protein (ab30146)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes

References

This product has been referenced in:
  • Hulmi JJ  et al. Prevention of chemotherapy-induced cachexia by ACVR2B ligand blocking has different effects on heart and skeletal muscle. J Cachexia Sarcopenia Muscle 9:417-432 (2018). IHC-P ; Mouse . Read more (PubMed: 29230965) »
  • Luo X  et al. Choroidal pericytes promote subretinal fibrosis after experimental photocoagulation. Dis Model Mech 11:N/A (2018). Read more (PubMed: 29622551) »
See all 11 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your reply. A quotation has been sent to you in a separate email, the proforma reference number is : *****. DISCOUNT CODE for ab1667 to be tested in Flow Cytometry : ******** Expiration date: DD MM YYYY I am very pleased to hear you would like to accept our offer and test ab1667 in Flow Cytometry. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forFlow Cytometryand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you very much for your interest in our anti-CCR3 antibodies. To our knowledge, none of ouranti-CCR3 antibodieshas not been tested in Flow Cytometry as well as in IHC-P. Therefore, I can offer a discount off a future purchase if you buy an anti-CCR3 now, test it in IHC-P or Flow Cytometryand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. You couldbuy for example, ab16231 (https://www.abcam.com/ab16231)and test it in IHC-P, or an anti-CCR3 tested in IHC-P and test it in Flow Cytometry. The link to the list of thefour anti-CCR3 tested in IHC-P and humanis : https://www.abcam.com/index.html?pageconfig=searchresults&search=CCR3&pt=1&sk=app&sv=74&sn=IHC-P&l=3&fViewMore=1. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab***** in IHC-P or Flow Cytometry. I will then send a discount code. This code must be issued before purchasingthe antibody to be testedso please wait for my reply before ordering. 2. Purchase antibody to be tested either by phone, fax, or online (www.abcam.com). 3. Test it in IHC-P or Flow Cytometry. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibodyordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe tested antibodyturns out to be unsuitable for IHC-P or Flow Cytometry, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount. Regarding the anti-CCR10, I confirm that ab3904 has been testedand is guaranteed to work in Flow Cytometry, IHC-P and on Human samples.

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Answer

Thank you for your enquiry. Ab1667: Antigen Retrieval: Sections were treated with antigen retrieval solution (Dako # S-1700, pH 6.0) and steamed for 20 minutes followed by cooling for 20 minutes. Variations of the method will work successfully as shown in the following publication that has images as well: http://clincancerres.aacrjournals.org/cgi/content/full/11/7/2459 Ab1669: Antigen Retrieval: Sections were treated with antigen retrieval solution (Dako # S-1700, pH 6.0 or #S-3308, pH 8.0) and steamed for 20 minutes followed by cooling for 20 minutes. I hope that this information helps. Should you have any further questions then please do not hesitate to get back in touch with me.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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