Anti-CCR3 antibody (ab36827)
Key features and details
- Rabbit polyclonal to CCR3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CCR3 antibody
See all CCR3 primary antibodies -
Description
Rabbit polyclonal to CCR3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
ab36827 was raised against a 14 amino acid peptide from near the carboxy terminus of CCR3 (Human).
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Positive control
- IHC-P:Human spleen tissue. ICC/IF: Human spleen cells WB: Human spleen cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab36827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 43 kDa).
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| IHC-P | (1) |
Use a concentration of 10 µg/ml.
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| ICC/IF |
Use a concentration of 20 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 43 kDa). |
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IHC-P
Use a concentration of 10 µg/ml. |
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ICC/IF
Use a concentration of 20 µg/ml. |
Target
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Function
Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. -
Tissue specificity
In eosinophils as well as trace amounts in neutrophils and monocytes. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1232 Human
- Omim: 601268 Human
- SwissProt: P51677 Human
- Unigene: 506190 Human
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Alternative names
- B chemokine receptor antibody
- b-chemokine receptor antibody
- C C chemokine receptor type 3 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR3 antibody (ab36827)
ab36827 at 10µg/ml staining CCR3 in human spleen tissue by IHC
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Western blot - Anti-CCR3 antibody (ab36827)Lane 1 : Anti-CCR3 antibody (ab36827) at 1 µg/ml
Lanes 2-3 : Anti-CCR3 antibody (ab36827) at 2 µg/ml
Lanes 1-2 : Human spleen tissue lysates
Lane 3 : Human spleen tissue lysates with Blocking peptide
Predicted band size: 43 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-CCR3 antibody (ab36827)Immunofluorescence of CCR3 in Human Spleen cells using ab36827 at 20 ug/ml.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab36827 has been referenced in 3 publications.
- Xu C et al. High expression of Sonic hedgehog in allergic airway epithelia contributes to goblet cell metaplasia. Mucosal Immunol 11:1306-1315 (2018). PubMed: 29867080
- Lee R et al. Enhanced chemokine-receptor expression, function, and signaling in healthy African American and scleroderma-patient monocytes are regulated by caveolin-1. Fibrogenesis Tissue Repair 8:11 (2015). IHC . PubMed: 26322128
- Musante V et al. RANTES modulates the release of glutamate in human neocortex. J Neurosci 28:12231-40 (2008). PubMed: 19020017
