Anti-CCR3 antibody (ab36827)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-CCR3 antibody
See all CCR3 primary antibodies -
DescriptionRabbit polyclonal to CCR3
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
ab36827 was raised against a 14 amino acid peptide from near the carboxy terminus of CCR3 (Human).
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Positive control
- Human spleen cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS, pH 8 -
Concentration information loading... -
PurityIon Exchange Chromatography
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab36827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 43 kDa). | |
| IHC-P | Use a concentration of 10 µg/ml. | |
| ICC/IF | Use a concentration of 20 µg/ml. |
Target
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FunctionReceptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
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Tissue specificityIn eosinophils as well as trace amounts in neutrophils and monocytes.
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Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
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Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 1232 Human
- Entrez Gene: 12771 Mouse
- Entrez Gene: 117027 Rat
- Omim: 601268 Human
- SwissProt: P51677 Human
- SwissProt: P51678 Mouse
- SwissProt: O54814 Rat
- Unigene: 506190 Human
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Alternative names
- B chemokine receptor antibody
- b-chemokine receptor antibody
- C C chemokine receptor type 3 antibody
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Images
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Western blot - Anti-CCR3 antibody (ab36827)Lane 1 : Anti-CCR3 antibody (ab36827) at 1 µg/ml
Lanes 2-3 : Anti-CCR3 antibody (ab36827) at 2 µg/ml
Lanes 1-2 : Human spleen tissue lysates
Lane 3 : Human spleen tissue lysates with Blocking peptide
Predicted band size: 43 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR3 antibody (ab36827)ab36827 at 10µg/ml staining CCR3 in human spleen tissue by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-CCR3 antibody (ab36827)Immunofluorescence of CCR3 in Human Spleen cells using ab36827 at 20 ug/ml.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Lee R et al. Enhanced chemokine-receptor expression, function, and signaling in healthy African American and scleroderma-patient monocytes are regulated by caveolin-1. Fibrogenesis Tissue Repair 8:11 (2015). IHC . Read more (PubMed: 26322128) »
- Musante V et al. RANTES modulates the release of glutamate in human neocortex. J Neurosci 28:12231-40 (2008). Read more (PubMed: 19020017) »
