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  1. Link

    ccr3-antibody-ab36827.pdf

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Immunology Innate Immunity Chemokines Beta Chemokine Rec. (CCR)
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Anti-CCR3 antibody (ab36827)

  • Datasheet
Reviews (1)Q&A (2)References (3)

Product price, shipping and contact information

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR3 antibody (ab36827)
  • Western blot - Anti-CCR3 antibody (ab36827)
  • Immunocytochemistry/ Immunofluorescence - Anti-CCR3 antibody (ab36827)

Key features and details

  • Rabbit polyclonal to CCR3
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-CCR3 antibody [Y31] - Low endotoxin, Azide free (ab228341)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-CCR3 antibody
    See all CCR3 primary antibodies
  • Description

    Rabbit polyclonal to CCR3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    ab36827 was raised against a 14 amino acid peptide from near the carboxy terminus of CCR3 (Human).

  • Positive control

    • IHC-P:Human spleen tissue. ICC/IF: Human spleen cells WB: Human spleen cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Ion Exchange Chromatography
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokine Rec. (CCR)
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV-related

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab36827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 43 kDa).
IHC-P (1)
Use a concentration of 10 µg/ml.
ICC/IF
Use a concentration of 20 µg/ml.
Notes
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 43 kDa).
IHC-P
Use a concentration of 10 µg/ml.
ICC/IF
Use a concentration of 20 µg/ml.

Target

  • Function

    Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
  • Tissue specificity

    In eosinophils as well as trace amounts in neutrophils and monocytes.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P51677 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1232 Human
    • Omim: 601268 Human
    • SwissProt: P51677 Human
    • Unigene: 506190 Human
    • Alternative names

      • B chemokine receptor antibody
      • b-chemokine receptor antibody
      • C C chemokine receptor type 3 antibody
      • C C CKR 3 antibody
      • C-C chemokine receptor type 3 antibody
      • C-C CKR-3 antibody
      • CC chemokine receptor 3 antibody
      • CC chemokine receptor type 3 antibody
      • CC CKR 3 antibody
      • CC R3 antibody
      • CC-CKR-3 antibody
      • CCCKR3 antibody
      • CCR 3 antibody
      • CCR-3 antibody
      • CCR3 antibody
      • CCR3_HUMAN antibody
      • CD 193 antibody
      • CD193 antibody
      • CD193 antigen antibody
      • Chemokine (C C motif) receptor 3 antibody
      • chemokine (C-C motif) receptor 3 antibody
      • Chemokine (CC) receptor 1 like 2 antibody
      • Chemokine CC motif receptor 3 antibody
      • Chemokine receptor 3 antibody
      • CK R3 antibody
      • CKR 3 antibody
      • CKR3 antibody
      • CMKB R3 antibody
      • CMKBR 3 antibody
      • CMKBR1L2 antibody
      • CMKBR3 antibody
      • Eosinophil CC chemokine receptor 3 antibody
      • Eosinophil eotaxin receptor antibody
      • Eotaxin receptor antibody
      • Macrophage inflammatory protein 1 alpha receptor like 2 antibody
      • MGC102841 antibody
      • MIP1 Alpha RL2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR3 antibody (ab36827)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR3 antibody (ab36827)

      ab36827 at 10µg/ml staining CCR3 in human spleen tissue by IHC 

    • Western blot - Anti-CCR3 antibody (ab36827)
      Western blot - Anti-CCR3 antibody (ab36827)
      Lane 1 : Anti-CCR3 antibody (ab36827) at 1 µg/ml
      Lanes 2-3 : Anti-CCR3 antibody (ab36827) at 2 µg/ml

      Lanes 1-2 : Human spleen tissue lysates
      Lane 3 : Human spleen tissue lysates with Blocking peptide

      Predicted band size: 43 kDa
      Observed band size: 55 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-CCR3 antibody (ab36827)
      Immunocytochemistry/ Immunofluorescence - Anti-CCR3 antibody (ab36827)
      Immunofluorescence of CCR3 in Human Spleen cells using ab36827 at 20 ug/ml.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (3)

    Publishing research using ab36827? Please let us know so that we can cite the reference in this datasheet.

    ab36827 has been referenced in 3 publications.

    • Xu C  et al. High expression of Sonic hedgehog in allergic airway epithelia contributes to goblet cell metaplasia. Mucosal Immunol 11:1306-1315 (2018). PubMed: 29867080
    • Lee R  et al. Enhanced chemokine-receptor expression, function, and signaling in healthy African American and scleroderma-patient monocytes are regulated by caveolin-1. Fibrogenesis Tissue Repair 8:11 (2015). IHC . PubMed: 26322128
    • Musante V  et al. RANTES modulates the release of glutamate in human neocortex. J Neurosci 28:12231-40 (2008). PubMed: 19020017

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CCR3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako high pH buffer
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    Vector Casein as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    DR. Henry King

    Verified customer

    Submitted Aug 18 2017

    Question

    Guten Tag,



    ich benötige ein Angebot zu folgendem Produkten:



    Pos 1. Anti CCR3 - Antibody 100µg (ab36827) (ist dieses Produkt das selbe wie CCR3 Polyclonal Antibody)



    Pos2. Anti-Collagen – Antibody (COL-1) 50µl (ab90395)



    Bitte nennen Sie mir Preis, Frachtkosten und Lieferzeit.



    Danke im Voraus.



    Read More

    Abcam community

    Verified customer

    Asked on Sep 26 2012

    Answer

    Vielen Dank für Ihre Anfrage.

    Könnten Sie mir bitte noch mitteilen,mitwelchenCCR3 Polyclonal Antibody Sie den ab36827 vergleichen möchten, da wir eine sehr große Auswahl an polyklonalen Antikörpern haben?

    Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

    Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Sep 26 2012

    Question

    Weare interested inCCR3(36827)to knowthat are tested and guaranteed for WB, it´s enough to place an order. Because we are having problems with this one from another company.



    Thank you foryour helpand soon replay



    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Sep 17 2012

    Answer

    Thank you for your reply.

    I am happy to confirm that ab36827 is tested and guaranteed by our Abpromise to work in WB with human, mouse and rat samples.

    https://www.abcam.com/abpromise

    I can suggest to run a positive control (Human spleen cell lysate) along with your samples.

    Please feel free to contact me again with any further questions.

    Read More

    Abcam Scientific Support

    Answered on Sep 17 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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