Overview

  • Product name
  • Description
    Rabbit polyclonal to CCR3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    ab36827 was raised against a 14 amino acid peptide from near the carboxy terminus of CCR3 (Human).

  • Positive control
    • Human spleen cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab36827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 43 kDa).
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function
    Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
  • Tissue specificity
    In eosinophils as well as trace amounts in neutrophils and monocytes.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B chemokine receptor antibody
    • b-chemokine receptor antibody
    • C C chemokine receptor type 3 antibody
    • C C CKR 3 antibody
    • C-C chemokine receptor type 3 antibody
    • C-C CKR-3 antibody
    • CC chemokine receptor 3 antibody
    • CC chemokine receptor type 3 antibody
    • CC CKR 3 antibody
    • CC R3 antibody
    • CC-CKR-3 antibody
    • CCCKR3 antibody
    • CCR 3 antibody
    • CCR-3 antibody
    • CCR3 antibody
    • CCR3_HUMAN antibody
    • CD 193 antibody
    • CD193 antibody
    • CD193 antigen antibody
    • Chemokine (C C motif) receptor 3 antibody
    • chemokine (C-C motif) receptor 3 antibody
    • Chemokine (CC) receptor 1 like 2 antibody
    • Chemokine CC motif receptor 3 antibody
    • Chemokine receptor 3 antibody
    • CK R3 antibody
    • CKR 3 antibody
    • CKR3 antibody
    • CMKB R3 antibody
    • CMKBR 3 antibody
    • CMKBR1L2 antibody
    • CMKBR3 antibody
    • Eosinophil CC chemokine receptor 3 antibody
    • Eosinophil eotaxin receptor antibody
    • Eotaxin receptor antibody
    • Macrophage inflammatory protein 1 alpha receptor like 2 antibody
    • MGC102841 antibody
    • MIP1 Alpha RL2 antibody
    see all

Images

  • Lane 1 : Anti-CCR3 antibody (ab36827) at 1 µg/ml
    Lanes 2-3 : Anti-CCR3 antibody (ab36827) at 2 µg/ml

    Lanes 1-2 : Human spleen tissue lysates
    Lane 3 : Human spleen tissue lysates with Blocking peptide

    Predicted band size: 43 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?

  • ab36827 at 10µg/ml staining CCR3 in human spleen tissue by IHC 

  • Immunofluorescence of CCR3 in Human Spleen cells using ab36827 at 20 ug/ml.

References

This product has been referenced in:
  • Lee R  et al. Enhanced chemokine-receptor expression, function, and signaling in healthy African American and scleroderma-patient monocytes are regulated by caveolin-1. Fibrogenesis Tissue Repair 8:11 (2015). IHC . Read more (PubMed: 26322128) »
  • Musante V  et al. RANTES modulates the release of glutamate in human neocortex. J Neurosci 28:12231-40 (2008). Read more (PubMed: 19020017) »
See all 2 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako high pH buffer
Permeabilization
No
Specification
Spleen
Blocking step
Vector Casein as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde

Dr. Henry King

Verified customer

Submitted Aug 18 2017

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