Overview

  • Product name
    Anti-CCR3 antibody [Y31]
    See all CCR3 primary antibodies
  • Description
    Rabbit monoclonal [Y31] to CCR3
  • Host species
    Rabbit
  • Specificity

    The mouse and rat recommendation is for WB use only. We do not guarantee IHC-P for mouse and rat.

  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Macaque monkey
  • Immunogen

    Synthetic peptide within Human CCR3 (C terminal). The exact sequence is proprietary.

  • Positive control
    • K562 and Daudi cell lysates and human liver tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 43 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
    • Tissue specificity
      In eosinophils as well as trace amounts in neutrophils and monocytes.
    • Sequence similarities
      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • B chemokine receptor antibody
      • b-chemokine receptor antibody
      • C C chemokine receptor type 3 antibody
      • C C CKR 3 antibody
      • C-C chemokine receptor type 3 antibody
      • C-C CKR-3 antibody
      • CC chemokine receptor 3 antibody
      • CC chemokine receptor type 3 antibody
      • CC CKR 3 antibody
      • CC R3 antibody
      • CC-CKR-3 antibody
      • CCCKR3 antibody
      • CCR 3 antibody
      • CCR-3 antibody
      • CCR3 antibody
      • CCR3_HUMAN antibody
      • CD 193 antibody
      • CD193 antibody
      • CD193 antigen antibody
      • Chemokine (C C motif) receptor 3 antibody
      • chemokine (C-C motif) receptor 3 antibody
      • Chemokine (CC) receptor 1 like 2 antibody
      • Chemokine CC motif receptor 3 antibody
      • Chemokine receptor 3 antibody
      • CK R3 antibody
      • CKR 3 antibody
      • CKR3 antibody
      • CMKB R3 antibody
      • CMKBR 3 antibody
      • CMKBR1L2 antibody
      • CMKBR3 antibody
      • Eosinophil CC chemokine receptor 3 antibody
      • Eosinophil eotaxin receptor antibody
      • Eotaxin receptor antibody
      • Macrophage inflammatory protein 1 alpha receptor like 2 antibody
      • MGC102841 antibody
      • MIP1 Alpha RL2 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded human liver tissue using ab32512 at 1/100 dilution.
    • All lanes : Anti-CCR3 antibody [Y31] (ab32512) at 1/500 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Daudi cell lysate

      Predicted band size: 43 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:
    • Kuse Y  et al. CCR3 Is Associated with the Death of a Photoreceptor Cell-line Induced by Light Exposure. Front Pharmacol 8:207 (2017). WB ; Mouse . Read more (PubMed: 28458639) »
    • Lee YS  et al. Crosstalk between CCL7 and CCR3 promotes metastasis of colon cancer cells via ERK-JNK signaling pathways. Oncotarget 7:36842-36853 (2016). Flow Cyt . Read more (PubMed: 27167205) »
    See all 13 Publications for this product

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

    Filter by Ratings

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Jurkat cells)
    Loading amount
    100000 cells
    Specification
    Jurkat cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted May 25 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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