Product nameAnti-CCR3 antibody [Y31] (HRP)
See all CCR3 primary antibodies
DescriptionRabbit monoclonal [Y31] to CCR3 (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Macaque monkey
Synthetic peptide within Human CCR3 (C terminal). The exact sequence is proprietary.
- WB: K562 and Daudi whole cell lysates.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab200020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 51 kDa (predicted molecular weight: 43 kDa).|
FunctionReceptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
Tissue specificityIn eosinophils as well as trace amounts in neutrophils and monocytes.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- B chemokine receptor antibody
- b-chemokine receptor antibody
- C C chemokine receptor type 3 antibody
All lanes : Anti-CCR3 antibody [Y31] (HRP) (ab200020) at 1/5000 dilution
Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 2 : Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab200020 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab200020 has not yet been referenced specifically in any publications.