Anti-CCR4 antibody (ab1669)
- Datasheet
- References (12)
- Protocols
Overview
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Product name
Anti-CCR4 antibody
See all CCR4 primary antibodies -
Description
Goat polyclonal to CCR4 -
Host species
Goat -
Specificity
Peptide sequence is < 50 % identical to other human chemokine receptors in this region. -
Tested applications
Suitable for: Flow Cyt, ICC/IF, ELISA, ICC, IHC-P, WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Human, Cynomolgus monkey -
Immunogen
Synthetic peptide:
SNYYLYESIPKPCTKEGIKAFGE
, corresponding to amino acids 17-39 of Human CCR4 (extracellular domain). -
Positive control
- Human peripheral blood cells or paraffin sections of spleen. IHC-P:FFPE mouse lymph node normal. IHC-P:FFPE human tonsil normal. IHC-P:FFPE rat spleen normal.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab1669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| ICC/IF | Use at an assay dependent concentration. Fix cells with 4% PFA (see Ritter et al). | |
| ELISA | 1/100000. | |
| ICC | 1/300. | |
| IHC-P | 1/300. | |
| WB | 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 42 kDa). | |
| IHC-Fr | Use at an assay dependent concentration. Fix in acetone at -20°C for 5 minutes. See Heller et al. |
Target
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Function
High affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival. -
Tissue specificity
Predominantly expressed in the thymus, in peripheral blood leukocytes, including T-cells, mostly CD4+ cells, and basophils, and in platelets; at lower levels, in the spleen and in monocytes. Detected also in macrophages, IL-2-activated natural killer cells and skin-homing memory T-cells, mostly the ones expressing the cutaneous lymphocyte antigen (CLA). Expressed in brain microvascular and coronary artery endothelial cells. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsIn natural killer cells, CCL22 binding induces phosphorylation on yet undefined Ser/Thr residues, most probably by beta-adrenergic receptor kinases 1 and 2. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1233 Human
- Entrez Gene: 12773 Mouse
- GenBank: NP_005499.1 Human
- Omim: 604836 Human
- SwissProt: P51679 Human
- SwissProt: P51680 Mouse
- Unigene: 184926 Human
- Unigene: 1337 Mouse
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Alternative names
- C C chemokine receptor type 4 antibody
- C C CKR 4 antibody
- C-C chemokine receptor type 4 antibody
see all
Images
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Flow Cytometry - Anti-CCR4 antibody (ab1669)This image is courtesy of an anonymous Abreviewab1669 staining CCR4 in Mouse whole blood by Flow Cytometry. Red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide and fixed in paraformaldehyde. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C. A biotin-conjugated Donkey anti-goat IgG polyclonal (1/200) was used as the secondary antibody.
Gating Strategy: Live Lymphocytes. -
Immunohistochemistry (Frozen sections) - Anti-CCR4 antibody (ab1669)Tan Y et al., PLoS One, 10, e0125073, 2015 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Frozen sections of human skin tissue stained for CCR4 using ab1669 in immunohistochemical analysis (green). CD31(red) and nuclei (blue) are also shown. The right hand panel is a merged image.
Image courtesy of PMID 25915746 (PLoS One 2015 Apr 27;10(4)).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR4 antibody (ab1669)Immunohistochemistry using ab1669 on a section of human spleen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR4 antibody (ab1669)IHC image of CCR4 staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1669, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-CCR4 antibody (ab1669)Anti-CCR4 antibody (ab1669) at 1 µg/ml + Human thymus tissue lysate - total protein (ab30146) at 10 µg
Secondary
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
CCR4 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Protocols
References
This product has been referenced in:
- Yuan FY et al. Tanshinone IIA improves diabetes mellitus via the NF-?B-induced AMPK signal pathway. Exp Ther Med 16:4225-4231 (2018). Read more (PubMed: 30344697) »
- Wang Y et al. HIC1 deletion promotes breast cancer progression by activating tumor cell/fibroblast crosstalk. J Clin Invest 128:5235-5250 (2018). Read more (PubMed: 30204129) »
