Product nameAnti-CCR4 antibody
See all CCR4 primary antibodies
DescriptionRabbit polyclonal to CCR4
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
- This antibody gave a positive signal in the following Mouse lysates: Thymus Tissue, Spleen Tissue and RAW 264.7 Whole Cell.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab47553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 41 kDa).|
FunctionHigh affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival.
Tissue specificityPredominantly expressed in the thymus, in peripheral blood leukocytes, including T-cells, mostly CD4+ cells, and basophils, and in platelets; at lower levels, in the spleen and in monocytes. Detected also in macrophages, IL-2-activated natural killer cells and skin-homing memory T-cells, mostly the ones expressing the cutaneous lymphocyte antigen (CLA). Expressed in brain microvascular and coronary artery endothelial cells.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
modificationsIn natural killer cells, CCL22 binding induces phosphorylation on yet undefined Ser/Thr residues, most probably by beta-adrenergic receptor kinases 1 and 2.
Cellular localizationCell membrane.
- Information by UniProt
- C C chemokine receptor type 4 antibody
- C C CKR 4 antibody
- C-C chemokine receptor type 4 antibody
All lanes : Anti-CCR4 antibody (ab47553) at 1 µg/ml
Lane 1 : Thymus (Mouse) Tissue Lysate
Lane 2 : Spleen (Mouse) Tissue Lysate
Lane 3 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 32 kDa. We are unsure as to the identity of these extra bands.
CCR4 contains a number of potential glycosylation sites so it is thought that this is the reason it runs at 55kDa.
ab47553 has not yet been referenced specifically in any publications.