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Immunology Innate Immunity Chemokines Beta Chemokine Rec. (CCR)
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Anti-CCR4 antibody [KH-4F5] (ab59550)

  • Datasheet
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Abpromise

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Immunocytochemistry/ Immunofluorescence - Anti-CCR4 antibody [KH-4F5] (ab59550)
  • Flow Cytometry - Anti-CCR4 antibody [KH-4F5] (ab59550)

Key features and details

  • Mouse monoclonal [KH-4F5] to CCR4
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG2a

You may also be interested in

Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Protein
Recombinant Human CCR4 protein (ab253173)

View more associated products

Overview

  • Product name

    Anti-CCR4 antibody [KH-4F5]
    See all CCR4 primary antibodies
  • Description

    Mouse monoclonal [KH-4F5] to CCR4
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide from human CCR4

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    KH-4F5
  • Isotype

    IgG2a
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokine Rec. (CCR)
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Chemokines

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant Human CCR4 protein (ab253173)

Applications

Our Abpromise guarantee covers the use of ab59550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1.2µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use 1µg for 106 cells.

Target

  • Function

    High affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival.
  • Tissue specificity

    Predominantly expressed in the thymus, in peripheral blood leukocytes, including T-cells, mostly CD4+ cells, and basophils, and in platelets; at lower levels, in the spleen and in monocytes. Detected also in macrophages, IL-2-activated natural killer cells and skin-homing memory T-cells, mostly the ones expressing the cutaneous lymphocyte antigen (CLA). Expressed in brain microvascular and coronary artery endothelial cells.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    In natural killer cells, CCL22 binding induces phosphorylation on yet undefined Ser/Thr residues, most probably by beta-adrenergic receptor kinases 1 and 2.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P51679 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1233 Human
    • GenBank: NP_005499.1 Human
    • Omim: 604836 Human
    • SwissProt: P51679 Human
    • Unigene: 184926 Human
    • Alternative names

      • C C chemokine receptor type 4 antibody
      • C C CKR 4 antibody
      • C-C chemokine receptor type 4 antibody
      • C-C CKR-4 antibody
      • CC CKR 4 antibody
      • CC-CKR-4 antibody
      • CCR 4 antibody
      • CCR-4 antibody
      • CCR4 antibody
      • CCR4_HUMAN antibody
      • CD194 antibody
      • Chemokine (CC motif) receptor 4 antibody
      • chemokine C C motif receptor 4 antibody
      • ChemR13 antibody
      • CKR4 antibody
      • CMKBR 4 antibody
      • CMKBR4 antibody
      • HGCN 14099 antibody
      • K5 5 antibody
      • K5-5 antibody
      • MGC88293 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CCR4 antibody [KH-4F5] (ab59550)
      Immunocytochemistry/ Immunofluorescence - Anti-CCR4 antibody [KH-4F5] (ab59550)
      Spectral Confocal Microscopy of CHO cells using ab59550 at 1 µg/106 cells . CHO cells were transiently transfected with an expression vector encoding CCR4. Binding of ab59550 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
    • Flow Cytometry - Anti-CCR4 antibody [KH-4F5] (ab59550)
      Flow Cytometry - Anti-CCR4 antibody [KH-4F5] (ab59550)
      FACS analysis of BOSC23 cells using ab59550 at 1.2 µg/ 106 cells. BOSC23 cells were transiently transfected with an expression vector encodingeither CCR4 (red curve) or an irrelevant protein(control transfectant). Binding of ab59550 was detected with a PE conjugated secondary antibody.

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab59550? Please let us know so that we can cite the reference in this datasheet.

    ab59550 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Immunohistochemistry (Frozen sections) abreview for Anti-CCR4 antibody [KH-4F5]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Frozen Section of Spleen, Tonsil and Normal Skin)
    Permeabilization
    Yes - 0.3% Triton X-100
    Specification
    Frozen Section of Spleen, Tonsil and Normal Skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Fixative
    Acetone
    Read More

    Milin Rana

    Verified customer

    Submitted May 30 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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