Anti-CCR4 antibody [KH-4F5] (ab59550)
![Immunocytochemistry/ Immunofluorescence - Anti-CCR4 antibody [KH-4F5] (ab59550)](https://www.abcam.com/ps/products/59/ab59550/Images/ab59550-73560-anti-ccr4-antibody-kh-4f5-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CCR4 antibody [KH-4F5] (ab59550)")
Key features and details
- Mouse monoclonal [KH-4F5] to CCR4
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-CCR4 antibody [KH-4F5]
See all CCR4 primary antibodies -
Description
Mouse monoclonal [KH-4F5] to CCR4 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide from human CCR4
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.20
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
KH-4F5 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab59550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 1.2µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| ICC/IF | Use 1µg for 106 cells. |
Target
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Function
High affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival. -
Tissue specificity
Predominantly expressed in the thymus, in peripheral blood leukocytes, including T-cells, mostly CD4+ cells, and basophils, and in platelets; at lower levels, in the spleen and in monocytes. Detected also in macrophages, IL-2-activated natural killer cells and skin-homing memory T-cells, mostly the ones expressing the cutaneous lymphocyte antigen (CLA). Expressed in brain microvascular and coronary artery endothelial cells. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsIn natural killer cells, CCL22 binding induces phosphorylation on yet undefined Ser/Thr residues, most probably by beta-adrenergic receptor kinases 1 and 2. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1233 Human
- GenBank: NP_005499.1 Human
- Omim: 604836 Human
- SwissProt: P51679 Human
- Unigene: 184926 Human
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Alternative names
- C C chemokine receptor type 4 antibody
- C C CKR 4 antibody
- C-C chemokine receptor type 4 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CCR4 antibody [KH-4F5] (ab59550)Spectral Confocal Microscopy of CHO cells using ab59550 at 1 µg/106 cells . CHO cells were transiently transfected with an expression vector encoding CCR4. Binding of ab59550 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
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![Flow Cytometry - Anti-CCR4 antibody [KH-4F5] (ab59550)](https://www.abcam.com/ps/products/59/ab59550/Images/ab59550-73559-anti-ccr4-antibody-kh-4f5-flow-cytometry.jpg)
Flow Cytometry - Anti-CCR4 antibody [KH-4F5] (ab59550)FACS analysis of BOSC23 cells using ab59550 at 1.2 µg/ 106 cells. BOSC23 cells were transiently transfected with an expression vector encodingeither CCR4 (red curve) or an irrelevant protein(control transfectant). Binding of ab59550 was detected with a PE conjugated secondary antibody.
Protocols
Datasheets and documents
References (0)
ab59550 has not yet been referenced specifically in any publications.
