Anti-CCR4 antibody [KH-4F5] (ab59550)
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Overview
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Product nameAnti-CCR4 antibody [KH-4F5]
See all CCR4 primary antibodies -
DescriptionMouse monoclonal [KH-4F5] to CCR4
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Host speciesMouse
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Tested applicationsSuitable for: ELISA, Flow Cyt, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide from human CCR4
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: None
Constituents: PBS, pH 7.2 -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone numberKH-4F5
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IsotypeIgG2a
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab59550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/200 - 1/400. | |
| Flow Cyt | Use 1.2µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| ICC/IF | Use 1µg for 106 cells. |
Target
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FunctionHigh affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival.
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Tissue specificityPredominantly expressed in the thymus, in peripheral blood leukocytes, including T-cells, mostly CD4+ cells, and basophils, and in platelets; at lower levels, in the spleen and in monocytes. Detected also in macrophages, IL-2-activated natural killer cells and skin-homing memory T-cells, mostly the ones expressing the cutaneous lymphocyte antigen (CLA). Expressed in brain microvascular and coronary artery endothelial cells.
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Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
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Post-translational
modificationsIn natural killer cells, CCL22 binding induces phosphorylation on yet undefined Ser/Thr residues, most probably by beta-adrenergic receptor kinases 1 and 2. -
Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 1233 Human
- GenBank: NP_005499.1 Human
- Omim: 604836 Human
- SwissProt: P51679 Human
- Unigene: 184926 Human
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Alternative names
- C C chemokine receptor type 4 antibody
- C C CKR 4 antibody
- C-C chemokine receptor type 4 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-CCR4 antibody [KH-4F5] (ab59550)](https://www.abcam.com/ps/products/59/ab59550/Images/ab59550if.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCR4 antibody [KH-4F5] (ab59550)Spectral Confocal Microscopy of CHO cells using ab59550 at 1 µg/106 cells . CHO cells were transiently transfected with an expression vector encoding CCR4. Binding of ab59550 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue). -
![Flow Cytometry - Anti-CCR4 antibody [KH-4F5] (ab59550)](https://www.abcam.com/ps/products/59/ab59550/Images/ab59550FACS.jpg)
Flow Cytometry - Anti-CCR4 antibody [KH-4F5] (ab59550)FACS analysis of BOSC23 cells using ab59550 at 1.2 µg/ 106 cells. BOSC23 cells were transiently transfected with an expression vector encodingeither CCR4 (red curve) or an irrelevant protein(control transfectant). Binding of ab59550 was detected with a PE conjugated secondary antibody.
Datasheets and documents
References
ab59550 has not yet been referenced specifically in any publications.
