- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-CCR5 antibody
See all CCR5 primary antibodies -
DescriptionRabbit polyclonal to CCR5
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Host speciesRabbit
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SpecificityThis antibody recognises the N-terminal region of the chemokine receptor CCR5.
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Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide corresponding to Human CCR5 aa 6-20 (N terminal).
Sequence:SSPIYDINYYTSEPC
(Peptide available asab7876) -
Positive control
- THP-1 whole cell lysate
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General notesHIV and chemokine receptor
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
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Storage bufferPBS with 0.02% sodium azide
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Concentration information loading... -
PurityAffinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab7346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 20 µg/ml. | |
| WB | 1/1000 - 1/2000. Detects a band of approximately 40 kDa.Can be blocked with CCR5 peptide (ab7876). | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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FunctionReceptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.
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Tissue specificityHighly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung.
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Involvement in diseaseGenetic variation in CCR5 is associated with suseptibility to diabetes mellitus insulin-dependent type 22 (IDDM22) [MIM:612522]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
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Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
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Post-translational
modificationsSulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES. -
Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 1234 Human
- Omim: 601373 Human
- SwissProt: P51681 Human
- Unigene: 450802 Human
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Alternative names
- AM4 7 antibody
- C C chemokine receptor type 5 antibody
- C C CKR 5 antibody
see all
Images
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Western blot - Anti-CCR5 antibody (ab7346)Anti-CCR5 antibody (ab7346) at 1/1000 dilution + THP-1 whole cell lysate
Observed band size: 40 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry/ Immunofluorescence - Anti-CCR5 antibody (ab7346)Immunofluorescence of CCR5 in human lymph node tissue with ab7346 at 20 μg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR5 antibody (ab7346)Immunohistochemistry of CCR5 in human lymph node tissue with ab7346 at 2.5 μg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR5 antibody (ab7346)ab7346 at 20µg/ml staining CCR5 in human lymph node tissue by IHC
References
This product has been referenced in:
- Kuzmina A et al. Functional Mimetics of the HIV-1 CCR5 Co-Receptor Displayed on the Surface of Magnetic Liposomes. PLoS One 10:e0144043 (2015). Read more (PubMed: 26629902) »
- Levina V et al. Role of eotaxin-1 signaling in ovarian cancer. Clin Cancer Res 15:2647-56 (2009). IHC-P ; Human . Read more (PubMed: 19351767) »
Customer reviews and Q&As
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