- Datasheet
- Specific References (3)
- Protocols
Overview
-
Product nameAnti-CCR5 antibody
See all CCR5 primary antibodies -
DescriptionRabbit polyclonal to CCR5
-
Host speciesRabbit
-
SpecificityThis antibody recognises the N-terminal region of the chemokine receptor CCR5.
-
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
-
Species reactivityReacts with: Human
-
Immunogen
Synthetic peptide corresponding to Human CCR5 aa 6-20 (N terminal).
Sequence:SSPIYDINYYTSEPC
(Peptide available asab7876) -
Positive control
- THP-1 whole cell lysate
-
General notesHIV and chemokine receptor
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
-
Storage bufferPreservative: 0.02% Sodium azide
-
Concentration information loading... -
PurityAffinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab7346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 20 µg/ml. | |
| WB | 1/1000 - 1/2000. Detects a band of approximately 40 kDa.Can be blocked with CCR5 peptide (ab7876). | |
| ICC/IF | Use at an assay dependent concentration. |
Target
-
FunctionReceptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.
-
Tissue specificityHighly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung.
-
Involvement in diseaseGenetic variation in CCR5 is associated with suseptibility to diabetes mellitus insulin-dependent type 22 (IDDM22) [MIM:612522]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
-
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
-
Post-translational
modificationsSulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES. -
Cellular localizationCell membrane.
- Information by UniProt
-
Database links
- Entrez Gene: 1234 Human
- Omim: 601373 Human
- SwissProt: P51681 Human
- Unigene: 450802 Human
-
Alternative names
- AM4 7 antibody
- C C chemokine receptor type 5 antibody
- C C CKR 5 antibody
see all
Images
-
Western blot - Anti-CCR5 antibody (ab7346)Anti-CCR5 antibody (ab7346) at 1/1000 dilution + THP-1 whole cell lysate
Observed band size: 40 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry/ Immunofluorescence - Anti-CCR5 antibody (ab7346)Immunofluorescence of CCR5 in human lymph node tissue with ab7346 at 20 μg/mL.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR5 antibody (ab7346)Immunohistochemistry of CCR5 in human lymph node tissue with ab7346 at 2.5 μg/ml.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR5 antibody (ab7346)ab7346 at 20µg/ml staining CCR5 in human lymph node tissue by IHC
References
This product has been referenced in:
- Kuzmina A et al. Functional Mimetics of the HIV-1 CCR5 Co-Receptor Displayed on the Surface of Magnetic Liposomes. PLoS One 10:e0144043 (2015). Read more (PubMed: 26629902) »
- Levina V et al. Role of eotaxin-1 signaling in ovarian cancer. Clin Cancer Res 15:2647-56 (2009). IHC-P ; Human . Read more (PubMed: 19351767) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"