• Product name
  • Description
    Rabbit polyclonal to CCR5
  • Host species
  • Specificity
    This antibody recognises the N-terminal region of the chemokine receptor CCR5.
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human CCR5 aa 6-20 (N terminal).


    (Peptide available as ab7876)

  • Positive control
    • THP-1 whole cell lysate
  • General notes
    HIV and chemokine receptor


Our Abpromise guarantee covers the use of ab7346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 20 µg/ml.
WB 1/1000 - 1/2000. Detects a band of approximately 40 kDa.Can be blocked with CCR5 peptide (ab7876).
ICC/IF Use at an assay dependent concentration.


  • Function
    Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.
  • Tissue specificity
    Highly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung.
  • Involvement in disease
    Genetic variation in CCR5 is associated with suseptibility to diabetes mellitus insulin-dependent type 22 (IDDM22) [MIM:612522]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
    O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
    Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
    Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AM4 7 antibody
    • C C chemokine receptor type 5 antibody
    • C C CKR 5 antibody
    • C-C chemokine receptor type 5 antibody
    • C-C CKR-5 antibody
    • C-C motif chemokine receptor 5 A159A antibody
    • CC Chemokine Receptor 5 antibody
    • CC Chemokine Receptor Type 5 antibody
    • CC CKR 5 antibody
    • CC-CKR-5 antibody
    • CCCKR 5 antibody
    • CCCKR5 antibody
    • CCR 5 antibody
    • CCR-5 antibody
    • CCR5 antibody
    • CCR5 chemokine (C C motif) receptor 5 antibody
    • CCR5_HUMAN antibody
    • CD 195 antibody
    • CD195 antibody
    • CD195 Antigen antibody
    • Chemokine C C motif receptor 5 antibody
    • Chemokine receptor CCR5 antibody
    • CHEMR13 antibody
    • CKR 5 antibody
    • CKR5 antibody
    • CMKBR 5 antibody
    • CMKBR5 antibody
    • FLJ78003 antibody
    • HIV 1 Fusion Coreceptor antibody
    • HIV-1 fusion coreceptor antibody
    • HIV1 fusion coreceptor antibody
    • IDDM22 antibody
    • MIP-1 alpha receptor antibody
    see all


  • Anti-CCR5 antibody (ab7346) at 1/1000 dilution + THP-1 whole cell lysate

    Observed band size: 40 kDa (why is the actual band size different from the predicted?)

  • Immunofluorescence of CCR5 in human lymph node tissue with ab7346 at 20 μg/mL.

  • Immunohistochemistry of CCR5 in human lymph node tissue with ab7346 at 2.5 μg/ml.

  • ab7346 at 20µg/ml staining CCR5 in human lymph node tissue by IHC


This product has been referenced in:

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for contacting Abcam.

These peptides are very short fragments of the CCR5 protein and as such may not contain the gp120 binding site. You may wish to use the whole protein. While this is not currently available at Abcam...

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Thank you for contacting us with your question. I aligned the immunogen sequence SSPIYDINYYTSEPC against the HIV M:C (taxid 505186) protein database and no putative conserved domains were identified. I hope this information is helpful, but pleas...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionnaire and I appreciate the additional steps that you have performed in order to try and get this antibody to...

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Thank you again for your enquiry. Regarding ab4969, ab4957, and ab4958 (the peptides corresponding to the antibodies you indicated), they are all greater than 95% pure, and they were purified via HPLC. If you have any further questions, please conta...

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Thank you for your enquiry. For ab2001 and ab7346, the peptides are non-HPLC, which averages out to 60-70% purity. Ab2001 (the antibody) was immunoaffinity chromatography purified IgG, and ab7346 (CCR5 antibody) is purified IgG. I will have to get b...

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We unfortunately do not have a picture of a Western to share, but the expected band size using ab7346 is approximately 40 kDa. To get rid of the non-specific bands on your blot, I suggest decreasing the concentrations of the primary and secondary a...

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The Western blot was performed on THP-1 whole cell lysate.

This antibody has only been tested in Western blotting, and so we have no data on its us in flow cytometry.


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