Anti-CCR5 antibody (ab7346)
Key features and details
- Rabbit polyclonal to CCR5
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CCR5 antibody
See all CCR5 primary antibodies -
Description
Rabbit polyclonal to CCR5 -
Host species
Rabbit -
Specificity
This antibody recognises the N-terminal region of the chemokine receptor CCR5. -
Tested Applications & Species
Application Species ICC/IF HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide corresponding to Human CCR5 aa 6-20 (N terminal).
Sequence:SSPIYDINYYTSEPC
(Peptide available asab7876) -
Positive control
- THP-1 whole cell lysate
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General notes
HIV and chemokine receptorThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC/IF |
Human
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IHC-P |
Human
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WB |
Human
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Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 20 µg/ml.
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WB |
1/1000 - 1/2000. Detects a band of approximately 40 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use a concentration of 20 µg/ml. |
WB
1/1000 - 1/2000. Detects a band of approximately 40 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates. -
Tissue specificity
Highly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung. -
Involvement in disease
Genetic variation in CCR5 is associated with suseptibility to diabetes mellitus insulin-dependent type 22 (IDDM22) [MIM:612522]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsSulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1234 Human
- Omim: 601373 Human
- SwissProt: P51681 Human
- Unigene: 450802 Human
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Alternative names
- AM4 7 antibody
- C C chemokine receptor type 5 antibody
- C C CKR 5 antibody
see all
Images
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Immunohistochemistry of CCR5 in human lymph node tissue with ab7346 at 2.5 μg/ml.
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Anti-CCR5 antibody (ab7346) at 1/1000 dilution + THP-1 whole cell lysate
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Immunofluorescence of CCR5 in human lymph node tissue with ab7346 at 20 μg/mL.
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ab7346 at 20µg/ml staining CCR5 in human lymph node tissue by IHC
Protocols
Datasheets and documents
References (4)
ab7346 has been referenced in 4 publications.
- Kuzmina A et al. Functional Mimetics of the HIV-1 CCR5 Co-Receptor Displayed on the Surface of Magnetic Liposomes. PLoS One 10:e0144043 (2015). PubMed: 26629902
- Jiang AP et al. Human Mucosal Mast Cells Capture HIV-1 and Mediate Viral trans-Infection of CD4+ T Cells. J Virol 90:2928-37 (2015). PubMed: 26719250
- Levina V et al. Role of eotaxin-1 signaling in ovarian cancer. Clin Cancer Res 15:2647-56 (2009). IHC-P ; Human . PubMed: 19351767
- Dittmer A et al. Human mesenchymal stem cells induce E-cadherin degradation in breast carcinoma spheroids by activating ADAM10. Cell Mol Life Sci 66:3053-65 (2009). WB ; Human . PubMed: 19603142