Anti-CCR5 antibody (ab7346)

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Overview

  • Product name
    Anti-CCR5 antibody
    See all CCR5 primary antibodies
  • Description
    Rabbit polyclonal to CCR5
  • Host species
    Rabbit
  • Specificity
    This antibody recognises the N-terminal region of the chemokine receptor CCR5.
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human CCR5 aa 6-20 (N terminal).
    Sequence:

    SSPIYDINYYTSEPC


    (Peptide available as ab7876)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • THP-1 whole cell lysate
  • General notes
    HIV and chemokine receptor

Applications

Our Abpromise guarantee covers the use of ab7346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 20 µg/ml.
WB 1/1000 - 1/2000. Detects a band of approximately 40 kDa.Can be blocked with CCR5 peptide (ab7876).
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.
  • Tissue specificity
    Highly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung.
  • Involvement in disease
    Genetic variation in CCR5 is associated with suseptibility to diabetes mellitus insulin-dependent type 22 (IDDM22) [MIM:612522]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications
    Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
    O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
    Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
    Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • AM4 7 antibody
      • C C chemokine receptor type 5 antibody
      • C C CKR 5 antibody
      • C-C chemokine receptor type 5 antibody
      • C-C CKR-5 antibody
      • C-C motif chemokine receptor 5 A159A antibody
      • CC Chemokine Receptor 5 antibody
      • CC Chemokine Receptor Type 5 antibody
      • CC CKR 5 antibody
      • CC-CKR-5 antibody
      • CCCKR 5 antibody
      • CCCKR5 antibody
      • CCR 5 antibody
      • CCR-5 antibody
      • CCR5 antibody
      • CCR5 chemokine (C C motif) receptor 5 antibody
      • CCR5_HUMAN antibody
      • CD 195 antibody
      • CD195 antibody
      • CD195 Antigen antibody
      • Chemokine C C motif receptor 5 antibody
      • Chemokine receptor CCR5 antibody
      • CHEMR13 antibody
      • CKR 5 antibody
      • CKR5 antibody
      • CMKBR 5 antibody
      • CMKBR5 antibody
      • FLJ78003 antibody
      • HIV 1 Fusion Coreceptor antibody
      • HIV-1 fusion coreceptor antibody
      • HIV1 fusion coreceptor antibody
      • IDDM22 antibody
      • MIP-1 alpha receptor antibody
      see all

    Images

    • Western blot - Anti-CCR5 antibody (ab7346)
      Western blot - Anti-CCR5 antibody (ab7346)
      Anti-CCR5 antibody (ab7346) at 1/1000 dilution + THP-1 whole cell lysate

      Observed band size: 40 kDa
      why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-CCR5 antibody (ab7346)
      Immunocytochemistry/ Immunofluorescence - Anti-CCR5 antibody (ab7346)

      Immunofluorescence of CCR5 in human lymph node tissue with ab7346 at 20 μg/mL.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR5 antibody (ab7346)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR5 antibody (ab7346)

      Immunohistochemistry of CCR5 in human lymph node tissue with ab7346 at 2.5 μg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR5 antibody (ab7346)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR5 antibody (ab7346)

      ab7346 at 20µg/ml staining CCR5 in human lymph node tissue by IHC

    References

    This product has been referenced in:
    See all 3 Publications for this product

    Publishing research using ab7346? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-8 of 8 Abreviews or Q&A

    Question

    Good day   I am a postdoctoral scientist at the NIH and I am interested in purchasing one of your CCR5 peptides. However I am not sure which of the peptide products (ab92719, ab95861 or ab7876) might be more suited to my experiment.   I am working on anti-HIV targets and I want to test (via ELISAs) if my target inhibits the gp120-CCR5 binding. Could you recommend one of your CCR5 peptides (and corresponding anti-CCR5 antibody) that might be most suited for my application?   Thanks and regards

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    Answer

    Thank you for contacting Abcam.



    These peptides are very short fragments of the CCR5 protein and as such may not contain the gp120 binding site. You may wish to use the whole protein. While this is not currently available at Abcam, you may find this by searching biocompare.com These peptides (ab7876ab92719 and ab95861) are the immunizing antigens for antibodies ab7346, ab64620 and ab85837 respectively.



    None of the antibodies has yet been tested in ELISA. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.



    If you would be interested in this offer please let me know.



    The product pages for these antibodies are at:

    https://www.abcam.com/CCR5-antibody-ab7346.html



    https://www.abcam.com/CCR5-antibody-mAbcam-64620-ab64620.html



    https://www.abcam.com/CCR5-antibody-ab85837.html



    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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    Question

    Could you tell me if the epitope recognized by your CCR5 (ab7346) corresponding to amino acids 6-20 of Human CCR5 is also present in HIV Clade C population, please?

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    Answer

    Thank you for contacting us with your question. I aligned the immunogen sequence SSPIYDINYYTSEPC against the HIV M:C (taxid 505186) protein database and no putative conserved domains were identified. I hope this information is helpful, but please let me know if you have further questions.

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    Question

    BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No CCR5 band, only background bands. SAMPLE Human sample from blood derived Mature Dendritic cells. Purified protein using Pierce M-PER reagent. PRIMARY ANTIBODY Primary Antibody: Abcam rabbit polyclonal CCR5 at 1:1000, diluted with 3:1 with TBS-T and NAP-Blocker and incubated at 4C overnight. Wash 30 minutes with TBS-T Attempted second time at advice of abcam tech, using 5:1 dilution of TBS-T and NAP-blocker. Wash 30 minutes with TBS-T DETECTION METHOD Detected with 45 minute step of Invitrogen Streptavadin 1:2500, then 30 minute wash. Developed with Pierce NBT-BCIP solution at RT until bands develop. POSITIVE AND NEGATIVE CONTROLS USED None ANTIBODY STORAGE CONDITIONS Stored at +4C SAMPLE PREPARATION Used 1:1 Laemmli Sample Buffer that has 5% B-MERC. Heated sample for 5 minutes at 95C. AMOUNT OF PROTEIN LOADED 30 micrograms per lane ELECTROPHORESIS/GEL CONDITIONS GeneMate Express Polyacrylamide Mini-gels. 4-20% Express gels TRANSFER AND BLOCKING CONDITIONS Transfer: Transfered at 25V for 100 minutes onto PVDF membrane. Blocker: G-Biosciences NAP-Blocker 1:1 with TBS-Tween. Blocked for 30 minutes at RT. SECONDARY ANTIBODY [another company] goat anti-rabbit, diluted 1:2000 in 3:1 TBS-T and NAP-blocker. Incubated at RT for 2 hours. Wash 30 minutes with TBS-T Attempted second time using 5:1 dilution of TBS-T and NAP-blocker. Wash 30 minutes with TBS-T HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? I have changed the amount of blocking reagent in the Primary, secondary, and Streptavadin solutions from 3:1 TBS-T/NAP-Blocker to 5:1 TBS-T/NAP-Blocker with no different results.

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    Answer

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionnaire and I appreciate the additional steps that you have performed in order to try and get this antibody to work. I am certainly prepared to offer you a credit note against this purchase provided that the antibody was purchased within the past 90 days. If this is the case please email me details of the order including the order number and date of purchase. I look forward to hearing from you.

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    Question

    This email is additional information for my previous email whichi subject is "Question about antibody and peptid". I forgot to ask you how pure they are and which method these peptides have been purified by. Thank you for your cooperation. ab2001, HREALEKDIKTSYIMDHC ab7346, SSPIYDINYYTSEPC ab3430, MSDFDSNPFADPDLNNPC ab3346, MSAPAQPPAEGTEGAAPGC ab3347, MSATAATVPPAAPAGEGGC Thank you for your cooperation

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    Answer

    Thank you again for your enquiry. Regarding ab4969, ab4957, and ab4958 (the peptides corresponding to the antibodies you indicated), they are all greater than 95% pure, and they were purified via HPLC. If you have any further questions, please contact us again.

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    Question

    This email is additional information for my previous email whichi subject is "Question about antibody and peptid". I forgot to ask you how pure they are and which method these peptides have been purified by. Thank you for your cooperation. ab2001, HREALEKDIKTSYIMDHC ab7346, SSPIYDINYYTSEPC ab3430, MSDFDSNPFADPDLNNPC ab3346, MSAPAQPPAEGTEGAAPGC ab3347, MSATAATVPPAAPAGEGGC Thank you for your cooperation

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    Answer

    Thank you for your enquiry. For ab2001 and ab7346, the peptides are non-HPLC, which averages out to 60-70% purity. Ab2001 (the antibody) was immunoaffinity chromatography purified IgG, and ab7346 (CCR5 antibody) is purified IgG. I will have to get back to you regarding the other peptides. If you have any additional questions, please let us know.

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    Question

    DESCRIPTION OF THE PROBLEM Multiple bands DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES Can you send a pic of a WB indicating the correct size and location of the CCR5 band (s)?

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    Answer

    We unfortunately do not have a picture of a Western to share, but the expected band size using ab7346 is approximately 40 kDa. To get rid of the non-specific bands on your blot, I suggest decreasing the concentrations of the primary and secondary and also make sure that you run a no primary control. If you have any more questions, please contact us again.

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    Question

    Could you tell me what cell line was used as a source of antigen in Western Blotting? Was a cell lysate used?

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    Answer

    The Western blot was performed on THP-1 whole cell lysate.

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    Question

    Could you send me an example plot of human blood lymphocytes stained with your CCR5 antibody? (Flow Cytometry Plot)

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    Answer

    This antibody has only been tested in Western blotting, and so we have no data on its us in flow cytometry.

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