Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [IGX6946] to CCR5 - BSA and Azide free
- Suitable for: Functional Studies, ICC, Flow Cyt
- Reacts with: Human
Product nameAnti-CCR5 antibody [IGX6946] - BSA and Azide free
See all CCR5 primary antibodies
DescriptionRabbit monoclonal [IGX6946] to CCR5 - BSA and Azide free
Tested applicationsSuitable for: Functional Studies, ICC, Flow Cytmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
Recombinant fragment. This information is considered to be commercially sensitive.
- ICC: Human PBMC cells. FlowCyt: Human PBMC cells.
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituent: 100% PBS
Concentration information loading...
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
- Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
Our Abpromise guarantee covers the use of ab277508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
FunctionReceptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.
Tissue specificityHighly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung.
Involvement in diseaseGenetic variation in CCR5 is associated with suseptibility to diabetes mellitus insulin-dependent type 22 (IDDM22) [MIM:612522]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
modificationsSulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES.
Cellular localizationCell membrane.
- Information by UniProt
- AM4 7 antibody
- C C chemokine receptor type 5 antibody
- C C CKR 5 antibody
Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CCR5 with ab277508 at 1/50 dilution (Right panel) compared with a Rabbit monoclonal IgG (ab172730) (Left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab277508. Then stained with anti-CD4 conjugated to Alexa Fluor® 647.
Gated on viable cells.
Immunofluorescent analysis of 4% paraformaldehyde and 0.1% TritonX-100 human PBMC (peripheral blood mononuclear cell line) cells labeling CCR5 with ab277508 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplamic staining in subsets of human PBMC. Tubulin counterstain is an annti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution. Nuclear counterstain is DAPI.
A calcium dose-response assay was performed at Multispan Inc. (Hayward, CA) following their MULTISCREEN™ Calcium 1.0 No Wash Assay Kit protocol. Target overexpressing cells were seeded in a 384 well plate in HHBS buffer at a density between 3,000 to 9,000 cells/well. Cells were incubated with calcium buffer containing Fluo-8 loading dye and probenecid (supplied in the kit). To test for agonist activity, cells were injected with a known control agonist in the 19th second of incubation and calcium mobilization was monitored for a total of 180 seconds. Following a 15-minute rest period, the cells from agonist mode testing were treated with the same known control agonist, in the 19th second, and calcium mobilization was again monitored as outlined above to assay for antagonist activity. Fluorescent emissions were read at 525 nm with excitation at 490 nm in a FLIPR384 instrument (Molecular Devices). Data analysis and antibody figures were prepared utilizing GraphPad Prism 8.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab277508 has not yet been referenced specifically in any publications.