Key features and details
- Mouse monoclonal [T21/8] to CCR5
- Suitable for: WB, IP, ELISA, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CCR5 antibody [T21/8]
See all CCR5 primary antibodies
DescriptionMouse monoclonal [T21/8] to CCR5
Tested applicationsSuitable for: WB, IP, ELISA, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
- Human tonsil tissue. Normal Human peripheral blood cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab110103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.|
|IP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 20 µg/ml.|
|Flow Cyt||Use 0.5µg for 105-8 cells.
in a final volume of 100 ul.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionReceptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.
Tissue specificityHighly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung.
Involvement in diseaseGenetic variation in CCR5 is associated with suseptibility to diabetes mellitus insulin-dependent type 22 (IDDM22) [MIM:612522]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
modificationsSulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES.
Cellular localizationCell membrane.
- Information by UniProt
- AM4 7 antibody
- C C chemokine receptor type 5 antibody
- C C CKR 5 antibody
ab110103 at 20µg/ml staining CCR5 in formalin-fixed, paraffin-embedded Human tonsil tissue.
Staining of normal Human peripheral blood cells with 0.25µg of purified mouse IgG1, K isotype control (open histogram) or 0.25 µg of ab110103 (colored histogram), followed by biotin Anti-mMouse IgG and SAv-PE.
Cells in the lymphocyte gate were used for analysis.
ab110103 has been referenced in 1 publication.
- Huang R et al. Loss of Fas expression and high expression of HLA-E promoting the immune escape of early colorectal cancer cells. Oncol Lett 13:3379-3386 (2017). PubMed: 28521443