Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab78429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.75 µg/ml. Predicted molecular weight: 42 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionReceptor for a C-C type chemokine. Binds to MIP-3-alpha/LARC and subsequently transduces a signal by increasing the intracellular calcium ions level.
Tissue specificitySpleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- BN 1 antibody
- BN1 antibody
- C C chemokine receptor type 6 antibody
ab78429 at 0.5ug/ml staining CCR6 in human large intestine tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.
All lanes : Anti-CCR6 antibody (ab78429) at 0.75 µg/ml
Lane 1 : Tissue lysate prepared from Rat Thymus
Lane 2 : Whole cell lysate prepared from Human MCF7 cells
Lane 3 : Whole cell lysate prepared from Hela cells
Lane 4 : Cell lysate prepared from smmc cells
Lysates/proteins at 50 µg per lane.
All lanes : HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution
Predicted band size: 42 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Zhu CC et al. CCR6 promotes tumor angiogenesis via the AKT/NF-?B/VEGF pathway in colorectal cancer. Biochim Biophys Acta 1864:387-397 (2018). WB, IHC-P . Read more (PubMed: 29097259) »
- Diao R et al. In vitro chemokine (C-C motif) receptor 6-dependent non-inflammatory chemotaxis during spermatogenesis. Biol Res 51:12 (2018). Read more (PubMed: 29788995) »