Overview

  • Product name
  • Description
    Rabbit polyclonal to CCR6
  • Host species
    Rabbit
  • Specificity
    No cross reactivity with other proteins.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human CCR6 aa 358-374 (C terminal). Different from the related rat and mouse sequences by two amino acids
    Sequence:

    RQTSETADNDNASSFTM

Properties

Applications

Our Abpromise guarantee covers the use of ab78429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.75 µg/ml. Predicted molecular weight: 42 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Receptor for a C-C type chemokine. Binds to MIP-3-alpha/LARC and subsequently transduces a signal by increasing the intracellular calcium ions level.
  • Tissue specificity
    Spleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BN 1 antibody
    • BN1 antibody
    • C C chemokine receptor type 6 antibody
    • C C CKR 6 antibody
    • C-C chemokine receptor type 6 antibody
    • C-C CKR-6 antibody
    • CC chemokine receptor type 6 antibody
    • CC CKR 6 antibody
    • CC R6 antibody
    • CC-CKR-6 antibody
    • CCCKR6 antibody
    • CCR 6 antibody
    • CCR-6 antibody
    • Ccr6 antibody
    • CCR6_HUMAN antibody
    • CD 196 antibody
    • CD196 antibody
    • CD196 antigen antibody
    • Chemokine (C C motif) receptor 6 antibody
    • Chemokine (C C) receptor 6 antibody
    • Chemokine (CC motif) receptor 6 antibody
    • Chemokine (CC) receptor 6 antibody
    • Chemokine receptor 6 antibody
    • Chemokine receptor like 3 antibody
    • Chemokine receptor-like 3 antibody
    • CKR 6 antibody
    • CKR L3 antibody
    • CKR-L3 antibody
    • CKR6 antibody
    • CKRL3 antibody
    • CMKBR 6 antibody
    • CMKBR6 antibody
    • DCR 2 antibody
    • DCR2 antibody
    • DRY 6 antibody
    • DRY6 antibody
    • G protein coupled receptor 29 antibody
    • G-protein coupled receptor 29 antibody
    • GPR 29 antibody
    • GPR CY4 antibody
    • GPR-CY4 antibody
    • GPR29 antibody
    • GPRCY 4 antibody
    • GPRCY4 antibody
    • LARC receptor antibody
    • Seven transmembrane receptor lymphocyte 22 antibody
    • STRL 22 antibody
    • STRL22 antibody
    see all

Images

  • ab78429 at 0.5ug/ml staining CCR6 in human large intestine tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.

  • All lanes : Anti-CCR6 antibody (ab78429) at 0.75 µg/ml

    Lane 1 : Tissue lysate prepared from Rat Thymus
    Lane 2 : Whole cell lysate prepared from Human MCF7 cells
    Lane 3 : Whole cell lysate prepared from Hela cells
    Lane 4 : Cell lysate prepared from smmc cells

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution

    Predicted band size: 42 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:
  • Zhu CC  et al. CCR6 promotes tumor angiogenesis via the AKT/NF-?B/VEGF pathway in colorectal cancer. Biochim Biophys Acta 1864:387-397 (2018). WB, IHC-P . Read more (PubMed: 29097259) »
  • Diao R  et al. In vitro chemokine (C-C motif) receptor 6-dependent non-inflammatory chemotaxis during spermatogenesis. Biol Res 51:12 (2018). Read more (PubMed: 29788995) »
See all 6 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Mammary gland tumor/inflamatory infiltrate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA
Permeabilization
No
Specification
Mammary gland tumor/inflamatory infiltrate
Blocking step
Sérum and milk (one hour for each one) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 27°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 27 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 5 minute(s) · Concentration: 1% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate
Sample
Human Tissue sections (tonsil)
Specification
tonsil
Permeabilization
No
Fixative
Formaldehyde

Dr. R Berahovich

Verified customer

Submitted May 09 2014

Answer

I will be happy to send the ab1659 to you as a replacement for ab78429 however I did look at the LSBio antibody I think you are talking about, cat #LS-C83958 and they only predict that it will react with mouse tissue based on sequence homology. I have gone ahead and placed the order for receiving ab1659 as a replacement and your order number is*******.

Read More

Answer

I have looked over all the information that you sent and you have tried all the variations in protocol that I can think of to try and get ab78429 to work in IHC. I would be happy to send you a replacement antibody however I would just like to say that we do not guarantee ab1659 to work in mouse tissue. The data we have on it suggests that at best it will react weakly but it does not fall under our Abpromise. If you would still like to try the antibody I will be more than happy to send you a vial but I just wanted to let you know that we do not guarantee it to work in mouse tissue.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
For licensing inquiries, please contact partnerships@abcam.com

Sign up