Recombinant Anti-CCR6 antibody [EPR24085-145] (ab303672)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24085-145] to CCR6
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CCR6 antibody [EPR24085-145]
See all CCR6 primary antibodies -
Description
Rabbit monoclonal [EPR24085-145] to CCR6 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-Fr,IHC-P,IP or WB -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Human PBMC cell line. Flow Cyt: Human PBMC cell line.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24085-145 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab303672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
1/500.
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ICC/IF |
1/50.
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Notes |
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Flow Cyt
1/500. |
ICC/IF
1/50. |
Target
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Function
Receptor for a C-C type chemokine. Binds to MIP-3-alpha/LARC and subsequently transduces a signal by increasing the intracellular calcium ions level. -
Tissue specificity
Spleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1235 Human
- Omim: 601835 Human
- SwissProt: P51684 Human
- Unigene: 46468 Human
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Alternative names
- BN 1 antibody
- BN1 antibody
- C C chemokine receptor type 6 antibody
see all
Images
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC (human primary peripheral blood mononuclear cell) cells labeling CCR6 with ab303672 at 1/50 dilution (10.68 µg/mL), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing cytoplasmic staining in subsets of human PBMCs. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).
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Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CCR6 with ab303672 at 1/500 dilution (0.1 µg) (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control. Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab303672. Then stained with anti-CD14 conjugated to BV510. Gated on viable cells.
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Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CCR6 with ab303672 at 1/500 dilution (0.1 µg) (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab303672. Then stained with anti-CD19 conjugated to Alexa Fluor® 647. Gated on viable lymphocytes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab303672 has not yet been referenced specifically in any publications.