Anti-CCR6 antibody - N-terminal (ab137369)
Key features and details
- Rabbit polyclonal to CCR6 - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CCR6 antibody - N-terminal
See all CCR6 primary antibodies -
Description
Rabbit polyclonal to CCR6 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to a region within N-terminal amino acids 1-76 of Human CCR6 (Uniprot ID P51684).
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Positive control
- 293T, A431, H1299, HeLaS3 cell lines; HepG2, MOLT4, Raji whole cell lysates; Human DU-145 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 43 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris-EDTA buffer (pH8.0) is also possible.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 43 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris-EDTA buffer (pH8.0) is also possible. |
Target
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Function
Receptor for a C-C type chemokine. Binds to MIP-3-alpha/LARC and subsequently transduces a signal by increasing the intracellular calcium ions level. -
Tissue specificity
Spleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1235 Human
- Omim: 601835 Human
- SwissProt: P51684 Human
- Unigene: 46468 Human
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Alternative names
- BN 1 antibody
- BN1 antibody
- C C chemokine receptor type 6 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human DU-145 xenograft tissue labeling CCR6, using ab137369 at a 1/100 dilution.
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All lanes : Anti-CCR6 antibody - N-terminal (ab137369) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lane 3 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 43 kDa
12% SDS PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab137369 has been referenced in 2 publications.
- Chang KC et al. Stem cell characteristics promote aggressiveness of diffuse large B-cell lymphoma. Sci Rep 10:21342 (2020). PubMed: 33288848
- Ikeda S et al. Disruption of CCL20-CCR6 interaction inhibits metastasis of advanced cutaneous T-cell lymphoma. Oncotarget 7:13563-74 (2016). WB . PubMed: 26789110